Maternal-to-zygotic transition as a potential target for niclosamide during early embryogenesis

Vliet, Sara M.F.; Dasgupta, Subham; Sparks, Nicole R L; Kirkwood, Jay S.; Vollaro, Alyssa; Hur, Manhoi; Nieden, Nicole I. zur; Volz, David C.

doi:10.1016/j.taap.2019.114699

Cited by 17 publications

(10 citation statements)

References 57 publications

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“…Targeted metabolomics for polar, primary metabolites was accomplished as described 59 . Briefly, metabolites were separated with an I-class UPLC system (Waters) on a ZIC-pHILIC column (2.1 × 150 mm, 5 µM) (EMD Millipore) using two mobile phases: (A) 15 mM ammonium bicarbonate in water (pH adjusted to 9.6 with ammonium hydroxide), and (B) acetonitrile.…”

Section: Methodsmentioning

confidence: 99%

Symbiont population control by host-symbiont metabolic interaction in Symbiodiniaceae-cnidarian associations

et al. 2020

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In cnidarian-Symbiodiniaceae symbioses, algal endosymbiont population control within the host is needed to sustain a symbiotic relationship. However, the molecular mechanisms that underlie such population control are unclear. Here we show that a cnidarian host uses nitrogen limitation as a primary mechanism to control endosymbiont populations. Nitrogen acquisition and assimilation transcripts become elevated in symbiotic Breviolum minutum algae as they reach high-densities within the sea anemone host Exaiptasia pallida. These same transcripts increase in free-living algae deprived of nitrogen. Symbiotic algae also have an elevated carbon-to-nitrogen ratio and shift metabolism towards scavenging nitrogen from purines relative to free-living algae. Exaiptasia glutamine synthetase and glutamate synthase transcripts concomitantly increase with the algal endosymbiont population, suggesting an increased ability of the host to assimilate ammonium. These results suggest algal growth and replication in hospite is controlled by access to nitrogen, which becomes limiting for the algae as their population within the host increases.

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Section: Methodsmentioning

confidence: 99%

Symbiont population control by host-symbiont metabolic interaction in Symbiodiniaceae-cnidarian associations

et al. 2020

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“…Adult fish were kept at 28 °C and a pH of approximately 7.2 with a 14 h light and 10 hr dark cycle. Embryos were collected within 30 min of spawning, sorted, and synchronized before treatment according to previously described methods [ 74 , 75 ]. At 5 h post fertilization (hpf) (~30–50% epiboly), individual embryos were transferred to a 96-well plate (1 embryo per well; 8 embryos per plate; three replicate plates per exposure type) and either kept in unaltered E3 embryo media (5 mM NaCl, 0.17 mM KCl, 0.33 mM CaCl 2 , and 0.33 mM MgSO 4 at pH 7) or in E3-containing SS smoke solution at the indicated concentrations.…”

Section: Methodsmentioning

confidence: 99%

Embryonic Exposure to Cigarette Smoke Extract Impedes Skeletal Development and Evokes Craniofacial Defects in Zebrafish

Karmach

Madrid

Dasgupta

et al. 2022

IJMS

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Exposure to cigarette smoke represents the largest source of preventable death and disease in the United States. This may be in part due to the nature of the delayed harmful effects as well as the lack of awareness of the scope of harm presented by these products. The presence of “light” versions further clouds the harmful effects of tobacco products. While active smoking in expectant mothers may be reduced by educational and outreach campaigns, exposure to secondhand smoke is often involuntary yet may harm the developing embryo. In this study, we show that the main component of secondhand smoke, sidestream cigarette smoke, from several brands, including harm-reduction versions, triggered unsuccessful hatching at 3 dpf and reduced overall survival at 6 dpf in developing zebrafish. At non-lethal concentrations, craniofacial defects with different severity based on the cigarette smoke extract were noted by 6 dpf. All tested products, including harm-reduction products, significantly impacted cartilage formation and/or bone mineralization in zebrafish embryos, independent of whether the bones/cartilage formed from the mesoderm or neural crest. Together, these results in a model system often used to detect embryonic malformations imply that exposure of a woman to secondhand smoke while pregnant may lead to mineralization issues in the skeleton of her newborn, ultimately adding a direct in utero association to the increased fracture risk observed in children of mothers exposed to cigarette smoke.

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“…The targeted analysis of 184 polar metabolites was performed on a Waters TQ-XS triplequadrupole mass spectrometer coupled to an I-class UPLC system, and the MS was operated in selected reaction monitoring mode. 34 To delve deeper into the metabolic pathways, targeted LC− MS/MS analysis of different groups of metabolites was performed in the leaves from plants exposed to CNW-L, CNW-M, CNW-H, Kocide, CuSO 4 , and the control, each with four biological replicates. Frozen ground leaf tissues (∼100 mg) were extracted in 1 mL of 80% methanol containing 2% formic acid, followed by vortex, sonication, and centrifugation at 20 000g at 4 °C for 20 min at each step.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…The MS scan range was 50–1600 m / z with a 100 ms scan time, and MS/MS was acquired in a data-dependent fashion. The targeted analysis of 184 polar metabolites was performed on a Waters TQ-XS triple-quadrupole mass spectrometer coupled to an I-class UPLC system, and the MS was operated in selected reaction monitoring mode …”

Section: Materials and Methodsmentioning

confidence: 99%

Unraveling Metabolic and Proteomic Features in Soybean Plants in Response to Copper Hydroxide Nanowires Compared to a Commercial Fertilizer

Majumdar

Long

Kirkwood

et al. 2021

Environ. Sci. Technol.

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Mechanistic understanding of the interaction of copper-based nanomaterials with crops is crucial for exploring their application in precision agriculture and their implications on plant health. We investigated the biological response of soybean (Glycine max) plants to the foliar application of copper hydroxide nanowires (CNWs) at realistic exposure concentrations. A commercial copper based-fungicide (Kocide), dissolved copper ions, and untreated controls were used for comparison to identify unique features at physiological, cellular, and molecular levels. After 32 d of exposure to CNW (0.36, 1.8, and 9 mg CNW/plant), the newly developed tissues accumulated significantly high levels of Cu (18−60 μg/g) compared to Kocide (10 μg/g); however, the rate of Cu translocation from the site of CNW treatment to other tissues was slower compared to other Cu treatments. Like Kocide, CNW exposure at medium and high doses altered Co, Mn, Zn, and Fe accumulation in the tissues and enhanced photosynthetic activities. The proteomic and metabolomic analyses of leaves from CNW-treated soybean plants suggest a dose-dependent response, resulting in the activation of major biological processes, including photosynthesis, energy production, fatty acid metabolism, lignin biosynthesis, and carbohydrate metabolism. In contrast to CNW treatments, Kocide exposure resulted in increased oxidative stress response and amino acid metabolism activation.

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Maternal-to-zygotic transition as a potential target for niclosamide during early embryogenesis

Cited by 17 publications

References 57 publications

Symbiont population control by host-symbiont metabolic interaction in Symbiodiniaceae-cnidarian associations

Symbiont population control by host-symbiont metabolic interaction in Symbiodiniaceae-cnidarian associations

Embryonic Exposure to Cigarette Smoke Extract Impedes Skeletal Development and Evokes Craniofacial Defects in Zebrafish

Unraveling Metabolic and Proteomic Features in Soybean Plants in Response to Copper Hydroxide Nanowires Compared to a Commercial Fertilizer

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