Maternal synthesis of abscisic acid controls seed development and yield in Nicotiana plumbaginifolia

Frey, Anne; Godin, Benoit; Bonnet, Magda; Sotta, Bruno; Marion‐Poll, Annie

doi:10.1007/s00425-003-1180-7

Cited by 132 publications

(94 citation statements)

References 28 publications

(35 reference statements)

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“…Previous studies of ABAinsensitive mutants have shown how alterations to ABA-dependent processes impact seed maturation, dormancy, and embryo growth (Cheng et al, 2002;Frey et al, 2004). To investigate whether the ABA deficiency in aba1-7 affects the regulation of carotenoid biosynthetic and degradation genes, the expression of 50 genes from four pathways (MEP, carotenoid biosynthesis and degradation, and ABA synthesis) was assessed in the ABA-insensitive mutant abi5-7, a major ABI locus impacting developing seed (Supplemental Table S9; Finkelstein and Lynch, 2000;Bensmihen et al, 2002;Brocard et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

ZEAXANTHIN EPOXIDASE Activity Potentiates Carotenoid Degradation in Maturing Seed

et al. 2016

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Elucidation of the carotenoid biosynthetic pathway has enabled altering the composition and content of carotenoids in various plants, but to achieve desired nutritional impacts, the genetic components regulating carotenoid homeostasis in seed, the plant organ consumed in greatest abundance, must be elucidated. We used a combination of linkage mapping, genome-wide association studies (GWAS), and pathway-level analysis to identify nine loci that impact the natural variation of seed carotenoids in Arabidopsis (Arabidopsis thaliana). ZEAXANTHIN EPOXIDASE (ZEP) was the major contributor to carotenoid composition, with mutants lacking ZEP activity showing a remarkable 6-fold increase in total seed carotenoids relative to the wild type. Natural variation in ZEP gene expression during seed development was identified as the underlying mechanism for fine-tuning carotenoid composition, stability, and ultimately content in Arabidopsis seed. We previously showed that two CAROTENOID CLEAVAGE DIOXYGENASE enzymes, CCD1 and CCD4, are the primary mediators of seed carotenoid degradation, and here we demonstrate that ZEP acts as an upstream control point of carotenoid homeostasis, with ZEPmediated epoxidation targeting carotenoids for degradation by CCD enzymes. Finally, four of the nine loci/enzymatic activities identified as underlying natural variation in Arabidopsis seed carotenoids also were identified in a recent GWAS of maize (Zea mays) kernel carotenoid variation. This first comparison of the natural variation in seed carotenoids in monocots and dicots suggests a surprising overlap in the genetic architecture of these traits between the two lineages and provides a list of likely candidates to target for selecting seed carotenoid variation in other species.

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Section: Discussionmentioning

confidence: 99%

ZEAXANTHIN EPOXIDASE Activity Potentiates Carotenoid Degradation in Maturing Seed

et al. 2016

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“…Suberin layers also cover dormant buds in potato, and the winter dormant buds of woody perennials suggest a general importance in generating the optimal internal environment for dormant plant cells. Suberization is promoted by the dormancy-inducing hormone ABA (Barberon et al, 2016), which is synthesized by the mother plant and present at increased concentrations in seeds set at low temperature (Frey et al, 2004;Kendall et al, 2011). Therefore, suberin biosynthesis may be an important adaptation for dormancy in multiple plant structures in addition to seeds.…”

Section: Discussionmentioning

confidence: 99%

Awake1, an ABC-Type Transporter, Reveals an Essential Role for Suberin in the Control of Seed Dormancy

Fedi

O’Neill²,

Ménard³

et al. 2017

Plant Physiol.

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The mother plant plays an important dynamic role in the control of dormancy of her progeny seed in response to environmental signals. In order to further understand the mechanisms by which this dormancy control takes place in Arabidopsis (Arabidopsis thaliana), we conducted a forward genetic screen to isolate mutants that fail to enter dormancy in response to variation in temperature during seed set. We show that, for the first of these mutants, designated awake1, the maternal allele is required for entry into strongly dormant states and that awake1 mutants show seed phenotypes shown previously to be associated with the loss of suberin in the seed. We identify awake1 as an allele of ABCG20, an ATP-binding cassette transporter-encoding gene required for the transport of fatty acids during suberin deposition, and show that further suberin-deficient mutants have seed dormancy defects. Seed coat suberin composition is affected by temperature during seed maturation, but this response appears to be independent of ABCG20. We conclude that seed coat suberin is essential for seed dormancy imposition by low temperature and that the exclusion of oxygen and water from the seed by the suberin and tannin layers is important for dormancy imposition.

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“…The endogenous ABA levels in seed vary remarkably at each developmental stage (Karssen et al, 1983;Groot et al, 1991;Frey et al, 2004;Gazzarrini et al, 2004;Setha et al, 2004). In Arabidopsis, ABA is accumulated in immature siliques most abundantly at 10 DAF, and decreases gradually thereafter.…”

Section: Roles Of Cyp707as During Seed Developmentmentioning

confidence: 99%

CYP707A1 and CYP707A2, Which Encode Abscisic Acid 8′-Hydroxylases, Are Indispensable for Proper Control of Seed Dormancy and Germination in Arabidopsis

Okamoto

Kuwahara²,

Seo³

et al. 2006

Plant Physiology

486

383

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Endogenous abscisic acid (ABA) levels are regulated by both biosynthesis and catabolism of the hormone. ABA 8#-hydroxylase is considered to be the key catabolic enzyme in many physiological processes. We have previously identified that four members of the Arabidopsis (Arabidopsis thaliana) CYP707A gene family (CYP707A1 to CYP707A4) encode ABA 8#-hydroxylases, and that the cyp707a2 mutants showed an increase in ABA levels in dry and imbibed seeds. In this study, we showed that the cyp707a1 mutant accumulated ABA to higher levels in dry seeds than the cyp707a2 mutant. Expression analysis showed that the CYP707A1 was expressed predominantly during mid-maturation and was down-regulated during latematuration. Concomitantly, the CYP707A2 transcript levels increased from late-maturation to mature dry seed. Phenotypic analysis of single and double cyp707a mutants indicates that the CYP707A1 is important for reducing ABA levels during midmaturation. On the other hand, CYP707A2 is responsible for the regulation of ABA levels from late-maturation to germination. Moreover, CYP707A1 and CYP707A3 were also shown to be involved in postgermination growth. Spatial expression analysis suggests that CYP707A1 was expressed predominantly in embryo during mid-maturation, whereas CYP707A2 expression was detected in both embryo and endosperm from late-maturation to germination. Our results demonstrate that each CYP707A gene plays a distinct role during seed development and postgermination growth.

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Maternal synthesis of abscisic acid controls seed development and yield in Nicotiana plumbaginifolia

Cited by 132 publications

References 28 publications

ZEAXANTHIN EPOXIDASE Activity Potentiates Carotenoid Degradation in Maturing Seed

ZEAXANTHIN EPOXIDASE Activity Potentiates Carotenoid Degradation in Maturing Seed

Awake1, an ABC-Type Transporter, Reveals an Essential Role for Suberin in the Control of Seed Dormancy

CYP707A1 and CYP707A2, Which Encode Abscisic Acid 8′-Hydroxylases, Are Indispensable for Proper Control of Seed Dormancy and Germination in Arabidopsis

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