Maternal serum interleukin-6 concentrations in patients with preterm premature rupture of membranes and evidence of infection

Murtha, Amy P.; Greig, Phillip C.; Jimmerson, Cathy E; Roitman-Johnson, Bea; Allen, Jean; Herbert, William N.P.

doi:10.1016/s0002-9378(96)80033-0

Cited by 73 publications

(42 citation statements)

References 8 publications

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“…Peak lists were created with the Spectrum Mill data extractor program with the following parameters: scans with the same precursor 61.4 m/z were merged within a time frame of 615 s; precursor ions needed to have a minimum S/N of 25; charges up to a maximum of 7 were assigned to the precursor ion and the 12 C peak was also determined by the Data Extractor; MH1 comprised between 450 and 1000, and a scan time comprised between 10 and 300 min was used.…”

Section: Peptide/protein Identification and Data Analysismentioning

confidence: 99%

“…Different methods based on clinical evaluation [3] and biological tests have been developed to diagnose PROM, among which the detection of proteins such as prolactin [4], a-fetoprotein [5], diamine oxidase [6,7], insulin-like growth factor binding protein-1 (IGFBP-1) [7,8], human choriogonadotrophin [9], fetal fibronectin [10,11], cytokines [12], interleukin-6 [12], interleukin-18 [13], as well as decidual relaxins H1 and H2 proteins [14], usually determined by immunoassays. One of the problems encoun-tered with these proteins is that even if they are present in amniotic fluid (AF) at concentrations higher than those in the maternal plasma, the contamination of vaginal section sample by maternal blood can lead to false-positive results.…”

Section: Introductionmentioning

confidence: 99%

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Proteome analysis of human plasma and amniotic fluid by Off‐Gel™ isoelectric focusing followed by nano‐LC‐MS/MS

et al. 2006

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This paper presents a comparative proteomic analysis of human maternal plasma and amniotic fluid (AF) samples from the same patient at term of pregnancy in order to find specific AF proteins as markers of premature rupture of membranes, a complication frequently observed during pregnancy. Maternal plasma and the corresponding AF were immunodepleted in order to remove the six most abundant proteins before the systematic analysis of their protein composition. The protein samples were then fractionated by IEF Off-Gel electrophoresis (OGE), digested and analyzed with nano-LC-MS/MS separation, revealing a total of 73 and 69 proteins identified in maternal plasma and AF samples, respectively. The proteins identified in AF have been compared to those identified in the mother plasma as well as to the reference human plasma protein list reported by Anderson et al. (Mol. Cell. Proteomics 2004, 3, 311-326). This comparison showed that 26 proteins were exclusively present in AF and not in plasma among which 10 have already been described to be placenta or pregnancy specific. As a further validation of the method, plasma proteins fractionated by OGE and analysed by nano-LC-MS/MS have been compared to the Swiss 2-D PAGE reference map by reconstructing a map that matches 2-D gel and OGE experimental data. This representation shows that 36 of 49 reference proteins could be identified in both data sets, and that isoform shifts in pI are well conserved in the OGE data sets.

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Section: Peptide/protein Identification and Data Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Proteome analysis of human plasma and amniotic fluid by Off‐Gel™ isoelectric focusing followed by nano‐LC‐MS/MS

et al. 2006

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“…The noninvasive tests that are used in the detection of intrauterine infection are maternal serum C-reactive protein (CRP), white blood cell (WBC) count, and IL-6 measurements. These tests have relatively low sensitivity rates between 67 and 81% [5,6]. Therefore, a highly sensitive and specific noninvasive prenatal test is needed to predict fetal infection in PPROM pregnancies.…”

Section: Introductionmentioning

confidence: 99%

Serial Ultrasonographic Examination of the Fetal Thymus in the Prediction of Early Neonatal Sepsis in Preterm Premature Rupture of Membranes

Çetin

Cetin

Uludağ

et al. 2014

Gynecol Obstet Invest

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Background/Aims: To evaluate the diagnostic accuracy of fetal thymus transverse diameter (FTTD) in predicting fetal infection in preterm premature rupture of membranes (PPROM) and compare its accuracy with cord blood tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). Methods: Forty consecutive pregnancies complicated with PPROM between 26^1/7 and 36^6/7 gestational weeks were evaluated prospectively. Serial fetal ultrasonography follow-ups with 3-day intervals were performed beginning on the admission day. The FTTD was recorded on every ultrasonographic examination. Cord blood TNF-α and IL-6 values were measured after delivery. Results: FTTD was decreased below 5% according to nomograms compared to the initial measurement in 45% of all PPROM cases. Decreased FTTD had a sensitivity of 100%, specificity of 73%, positive predictive value of 55%, and negative predictive value of 100% in predicting early neonatal sepsis. Cord blood TNF-α had a sensitivity of 80% and specificity of 90%, whereas IL-6 had a sensitivity of 90% and specificity of 63.3% in predicting early neonatal sepsis. Conclusions: Assessment of the decrease in FTTD by serial ultrasonographic examinations is a promising ‘prenatal' method for the early detection of early neonatal sepsis.

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“…A simple and more repeatable method would be assessment of maternal serum inflammation markers: however there is still no consensus on the ideal marker as far as sensitivity and specificity is concerned [12,13,14,15,16,17]. …”

Section: Introductionmentioning

confidence: 99%

“…IL-6 is elevated in maternal serum in pPROM patients with chorioamnionitis and funisitis [17,18], but IL-6 values are not routinely assessed as this procedure does not represent a cost-effective option in clinical practice.…”

Section: Introductionmentioning

confidence: 99%

Maternal C-Reactive Protein at Hospital Admission Is a Simple Predictor of Funisitis in Preterm Premature Rupture of Membranes

Perrone¹,

Anceschi²,

Capri³

et al. 2012

Gynecol Obstet Invest

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Aim: To analyze the prognostic value of maternal serum C-reactive protein (CRP) in predicting funisitis in patients with preterm premature rupture of membranes (pPROM). Methods: 66 patients (gestational age 24–33 weeks) hospitalized 1–12 h after pPROM were enrolled. White blood cell count (WBC), platelet count (PLT) and plasma concentration of CRP were assessed every 3 days. Histological evidence of chorioamnionitis and funisitis was obtained post-partum. Receiver operating characteristic (ROC) curves were employed to evaluate the role of maternal CRP in predicting funisitis. Results: Funisitis was found in 24 patients (36.3%); 42 patients (63.7%) without funisitis were considered as controls. PLT and WBC at admission and before delivery did not show significant differences and were not statistically different between the two groups. Patients with funisitis had significantly higher CRP levels both at admission to hospital and 24– 48 h before delivery. ROC curve analysis showed that CRP at admission (area under the curve: 0.671, p = 0.021) and before delivery (area under the curve: 0.737, p = 0.001) are predictive of funisitis. Conclusions: High maternal serum CRP levels (>20,000 µg/l) in pPROM patients at admission to hospital may be an early marker which indicates, with a good diagnostic performance, the presence of funisitis.

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Maternal serum interleukin-6 concentrations in patients with preterm premature rupture of membranes and evidence of infection

Cited by 73 publications

References 8 publications

Proteome analysis of human plasma and amniotic fluid by Off‐Gel™ isoelectric focusing followed by nano‐LC‐MS/MS

Proteome analysis of human plasma and amniotic fluid by Off‐Gel™ isoelectric focusing followed by nano‐LC‐MS/MS

Serial Ultrasonographic Examination of the Fetal Thymus in the Prediction of Early Neonatal Sepsis in Preterm Premature Rupture of Membranes

Maternal C-Reactive Protein at Hospital Admission Is a Simple Predictor of Funisitis in Preterm Premature Rupture of Membranes

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