Maternal Rumen Bacteriota Shapes the Offspring Rumen Bacteriota, Affecting the Development of Young Ruminants

Jin, Shuwen; Zhang, Zhe; Zhang, Gonghai; He, Bo; Qin, Yilang; Yang, Bin; Yu, Zhongtang; Wang, Jiakun

doi:10.1128/spectrum.03590-22

Cited by 2 publications

(1 citation statement)

References 64 publications

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“…Most wild animals consume a variety of food types rather than focusing on a single type of food to meet nutritional demands. Furthermore, most current research on mammals has focused on humans ( 14 ), nonhuman primates ( 4 ), laboratory model animals such as mice ( 15 ), domestic animals including pigs, sheep, and cows ( 16 – 18 ), or captive animals ( 11 ) rather than wild animals. Conducting research on wild animals could provide valuable insights into the role of gut microbiome in natural conditions in terms of the evolution and adaptation of the host ( 1 ).…”

Section: Introductionmentioning

confidence: 99%

The role of host traits and geography in shaping the gut microbiome of insectivorous bats

Dai,

Leng,

et al. 2024

mSphere

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The gut microbiome is a symbiotic microbial community associated with the host and plays multiple important roles in host physiology, nutrition, and health. A number of factors have been shown to influence the gut microbiome, among which diet is considered to be one of the most important; however, the relationship between diet composition and gut microbiota in wild mammals is still not well recognized. Herein, we characterized the gut microbiota of bats and examined the effects of diet, host taxa, body size, gender, elevation, and latitude on the gut microbiota. The cytochrome C oxidase subunit I (COI) gene and 16S rRNA gene amplicons were sequenced from the feces of eight insectivorous bat species in southern China, including Miniopterus fuliginosus , Aselliscus stoliczkanus , Myotis laniger , Rhinolophus episcopus , Rhinolophus osgoodi , Rhinolophus ferrumequinum , Rhinolophus affinis, and Rhinolophus pusillus . The results showed that the composition of gut microbiome and diet exhibited significant differences among bat species. Diet composition and gut microbiota were significantly correlated at the order, family, genus, and operational taxonomic unit levels, while certain insects had a marked effect on the gut microbiome at specific taxonomic levels. In addition, elevation, latitude, body weight of bats, and host species had significant effects on the gut microbiome, but phylosymbiosis between host phylogeny and gut microbiome was lacking. These findings clarify the relationship between gut microbiome and diet and contribute to improving our understanding of host ecology and the evolution of the gut microbiome in wild mammals. IMPORTANCE The gut microbiome is critical for the adaptation of wildlife to the dynamic environment. Bats are the second-largest group of mammals with short intestinal tract, yet their gut microbiome is still poorly studied. Herein, we explored the relationships between gut microbiome and food composition, host taxa, body size, gender, elevation, and latitude. We found a significant association between diet composition and gut microbiome in insectivorous bats, with certain insect species having major impacts on gut microbiome. Factors like species taxa, body weight, elevation, and latitude also affected the gut microbiome, but we failed to detect phylosymbiosis between the host phylogeny and the gut microbiome. Overall, our study presents novel insights into how multiple factors shape the bat’s gut microbiome together and provides a study case on host-microbe interactions in wildlife.

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Section: Introductionmentioning

confidence: 99%

The role of host traits and geography in shaping the gut microbiome of insectivorous bats

Dai,

Leng,

et al. 2024

mSphere

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The Effects of Different Doses of 3-NOP on Ruminal Fermentation Parameters, Methane Production, and the Microbiota of Lambs In Vitro

Xuan,

Zheng,

et al. 2024

Fermentation

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3-Nitrooxypropanol (3-NOP) is a nitrooxy compound that specifically targets methyl-coenzyme M reductase (MCR), ultimately resulting in a reduction in methane production. In this study, we undertook an in vitro investigation of the effects of different dosages of 3-NOP on ruminal fermentation parameters, methane production, and the microbial community. A single-factor completely randomized design was adopted, comprising a control treatment (C), where no 3-NOP was added to the fermentation substrate, and three 3-NOP treatments, where 0.025 mg (low-dose treatment, LD), 0.05 mg (medium-dose treatment, MD), or 0.1 mg (high-dose treatment, HD) was added to 1 g of fermentation substrate (DM basis), followed by incubation for 24 h in vitro. The results showed that, compared with the control treatment, the three dosages of 3-NOP reduced total gas production, methane production, and acetate production (all p < 0.01). In contrast, 3-NOP treatment increased H2 production and the molar proportions of propionate and butyrate (all p ≤ 0.02), resulting in a decrease in the acetate-propionate ratio (p < 0.01). Meanwhile, the microbial profiles were not altered by the treatments, but the relative abundances of Prevotella, Methanobrevibacter, and Ophryoscolex were increased by the MD and HD treatments (all p < 0.01), whereas those of Methanosarcina, Methanosaeta, Sphaerochaeta, RFN20, Entodinium, and Diplodinium were decreased by the HD treatment (all p ≤ 0.03). Moreover, the results of a correlation analysis showed that there was a certain correlation between these microorganisms and total gas production, methane production, H2 production, acetate, propionate, and butyrate. In summary, under in vitro conditions, the addition of 3-NOP to the diet affected the microbial community structure, thereby altering the ruminal fermentation pattern and reducing methane production. Our results indicated that 0.05 mg per g of dietary DM is the recommended inclusion ratio for 3-NOP in the diet of lambs.

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Maternal Rumen Bacteriota Shapes the Offspring Rumen Bacteriota, Affecting the Development of Young Ruminants

Cited by 2 publications

References 64 publications

The role of host traits and geography in shaping the gut microbiome of insectivorous bats

The role of host traits and geography in shaping the gut microbiome of insectivorous bats

The Effects of Different Doses of 3-NOP on Ruminal Fermentation Parameters, Methane Production, and the Microbiota of Lambs In Vitro

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