Maternal predictors and quality of umbilical cord blood units

Bielec-Berek, Beata; Jastrzębska-Stojko, Żaneta; Drosdzol–Cop, Agnieszka; Jendyk, Cecylia; Boruczkowski, Dariusz; Ołdak, Tomasz; Nowak-Brzezińska, Agnieszka; Stojko, Rafał

doi:10.1007/s10561-017-9657-y

Cited by 4 publications

(3 citation statements)

References 19 publications

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“…Uji korelasi yang dilakukan untuk melihat 12 Pada kehamilan dengan usia ibu di atas 31 tahun, jumlah sel darah putih lebih banyak dibandingkan usia ibu di bawah 25 tahun. Sel punca darah tali pusat merupakan populasi sel yang berada pada sel darah putih.…”

Section: Hasilunclassified

Pengaruh Usia Ibu Hamil terhadap Jumlah Sel Punca Hematopoietik dan Very Small Embryonic-like Stem Cell pada Darah Tali Pusat

Chouw¹,

Putera²,

Sartika³

et al. 2019

ijcp

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AbstrakDarah tali pusat telah banyak digunakan dalam terapi regeneratif. Hal ini disebabkan darah tali pusat mengandung sel punca, yaitu sel punca hematopoietik dan very small embryonic-like stem cell. Keberhasilan terapi regeneratif menggunakan darah tali pusat membutuhkan dosis yang disesuaikan dengan berat badan dan cara pemberian sel punca. Oleh karena itu, dibutuhkan informasi yang dapat memengaruhi jumlah sel punca yang ada pada darah tali pusat. Usia ibu pada saat kehamilan diperkirakan memiliki pengaruh terhadap jumlah sel punca. Penelitian ini dilakukan dengan pendekatan studi potong lintang. Penelitian dilakukan di Laboratorium Prodia StemCell Indonesia pada bulan Desember 2017. Sebanyak 22 sampel yang digunakan dalam penelitian ini merupakan darah tali pusat yang telah disimpan sebelumnya selama lebih dari dua tahun. Deteksi jumlah sel hematopoietik dan very small embryoniclike stem cell dilakukan dengan menggunakan metode flowcytometry. Deteksi sel punca hematopoietik dilakukan dengan menggunakan molekul penanda permukaan CD34+ dan CD45+, sedangkan deteksi very small embryonic-like stem cell dilakukan dengan menggunakan molekul penanda permukaan CD45-, CD34+, dan CD184(CXCR4)+. Pada kelompok usia ibu di atas 30 tahun, rata-rata jumlah sel hematopoietik adalah 63,71±58.419x10 3 sel/mL dan rata-rata jumlah very small embryonic-like stem cell adalah 7,83±7.060 x10 3 sel/mL. Uji beda menunjukkan semakin tua usia ibu pada saat kehamilan, jumlah sel punca semakin meningkat, namun tidak ada perbedaan yang signifikan (r=0,0510; p<0,005). Hal ini menunjukkan bahwa usia ibu tidak memengaruhi konsentrasi sel darah tali pusat. Kata kunci: Darah tali pusat, sel hematopoietik, sel punca, very small embryonic-like stem cell AbstractUmbilical cord blood (UCB) has been used in regenerative medicine due to the stem cell content in the blood. Hematopoietic stem cell and very small embryonic-like stem cell are found in UCB sample. Dosage and route of administration of stem cell need to be determined for the success of regenerative therapy. Therefore, information that can affect the stem cell number is needed. This study used crosssectional approach, and was conducted at Prodia StemCell Laboratory in December 2017. Twenty-two UCB samples collected from Prodia StemCell Laboratory which had been stored for more than 2 years were thawed to detect the number of stem cell. Flowcytometry method was used to detect the number of hematopoietic stem cell and very small embryonic-like stem cell in UCB sample. Hematopoietic stem cell was detected using antibody CD34+ and CD45+ while very small embryonic-like stem cell was detected using CD45-, CD34+, and CD184(CXCR4)+. The mean cell number of hematopoietic stem cell and very small embryonic-like stem cell in maternal age above 30 years old group were 63.71 ±58.419x10 3 cell/mL and 7.83±7.060 x10 3 cell/mL, respectively. There was no significant difference in maternal age group during pregnancy (r=0.0510; p<0.005). In conclusion, the number of cells in umbilical cord blood due to the ...

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Section: Hasilunclassified

Pengaruh Usia Ibu Hamil terhadap Jumlah Sel Punca Hematopoietik dan Very Small Embryonic-like Stem Cell pada Darah Tali Pusat

Chouw¹,

Putera²,

Sartika³

et al. 2019

ijcp

View full text Add to dashboard Cite

show abstract

“…The effect of maternal and neonatal factors on the quality of umbilical cord blood stems cells have been a subject of numerous studies [ 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 ]. One of the deciding factors in the usefulness of cord blood is the number of CD34+ stem cells.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, in this study, the correlation between the mode of delivery, the age of the mother and the quality of the obtained material for transplantation was assessed. The older the women from whom umbilical cord blood was collected, the lower the mean concentration of HSC cells in the material [ 37 ]. Aufderhaar et al showed that perinatal factors such as low blood pH and prolonged first stage of labor correlate with increases in CD34+ cells and cord blood progenitor cells [ 45 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Impact of Pregnancy-Associated Factors on the Quality of Wharton’s Jelly-Derived Stem Cells Using SOX2 Gene Expression as a Marker

Gil-Kulik

Świstowska

Krzyżanowski

et al. 2022

IJMS

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SOX2 is a recognized pluripotent transcription factor involved in stem cell homeostasis, self-renewal and reprogramming. It belongs to, one of the SRY-related HMG-box (SOX) family of transcription factors, taking part in the regulation of embryonic development and determination of cell fate. Among other functions, SOX2 promotes proliferation, survival, invasion, metastasis, cancer stemness, and drug resistance. SOX2 interacts with other transcription factors in multiple signaling pathways to control growth and survival. The aim of the study was to determine the effect of a parturient’s age, umbilical cord blood pH and length of pregnancy on the quality of stem cells derived from Wharton’s jelly (WJSC) by looking at birth weight and using SOX2 gene expression as a marker. Using qPCR the authors, evaluated the expression of SOX2 in WJSC acquired from the umbilical cords of 30 women right after the delivery. The results showed a significant correlation between the birth weight and the expression of SOX2 in WJSC in relation to maternal age, umbilical cord blood pH, and the length of pregnancy. The authors observed that the younger the woman and the lower the umbilical cord blood pH, the earlier the delivery occurs, the lower the birth weight and the higher SOX2 gene expression in WJSC. In research studies and clinical applications of regenerative medicine utilizing mesenchymal stem cells derived from Wharton’s Jelly of the umbilical cord, assessment of maternal and embryonic factors influencing the quality of cells is critical.

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Increased Expression of BIRC2, BIRC3, and BIRC5 from the IAP Family in Mesenchymal Stem Cells of the Umbilical Cord Wharton’s Jelly (WJSC) in Younger Women Giving Birth Naturally

Gil-Kulik

Świstowska

Kondracka

et al. 2020

Oxidative Medicine and Cellular Longevity

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The knowledge of factors affecting the viability as well as proliferation and therapeutic potential of perinatal stem cells is of great importance for the decisions concerning their collection, multiplication, and storing. The aim of this work is to evaluate the expression of the BIRC2, BIRC3, and BIRC5 genes at the level of transcription in mesenchymal stem cells derived from the umbilical cord Wharton's jelly. The study examined the relationship between the expression level of the studied genes and selected biophysical parameters of umbilical blood: pH, pCO 2 , pO 2 , and cHCO 3 . Moreover, the relationship between the pregnant age, the type of delivery (natural delivery or cesarean section), and the level of expression of the BIRC2, BIRC3, and BIRC5 genes was assessed. The research was carried out on mesenchymal stem cells derived from the umbilical cord Wharton's jelly (WJSC) taken from 55 women immediately after delivery. Expression of the examined genes was assessed with the qPCR method using commercially available reagent kits. On the basis of the conducted research, it was demonstrated that WJSCs collected from younger women giving birth naturally, and in the acidic environment of the umbilical cord blood, are characterized by a higher expression of the BIRC2, BIRC3, and BIRC5 genes. It was shown that the expression of the BIRC2 and BIRC3 genes in Wharton's jelly mesenchymal stem cells declines with the mother's age. Our research suggests that stem cells collected from younger women giving birth naturally can be more resistant to apoptosis and show a more stem cell-like character, which can increase their therapeutic potential and clinical utility, but this conclusion needs to be approved in the next studies.

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Maternal predictors and quality of umbilical cord blood units

Cited by 4 publications

References 19 publications

Pengaruh Usia Ibu Hamil terhadap Jumlah Sel Punca Hematopoietik dan Very Small Embryonic-like Stem Cell pada Darah Tali Pusat

Pengaruh Usia Ibu Hamil terhadap Jumlah Sel Punca Hematopoietik dan Very Small Embryonic-like Stem Cell pada Darah Tali Pusat

Evaluation of the Impact of Pregnancy-Associated Factors on the Quality of Wharton’s Jelly-Derived Stem Cells Using SOX2 Gene Expression as a Marker

Increased Expression of BIRC2, BIRC3, and BIRC5 from the IAP Family in Mesenchymal Stem Cells of the Umbilical Cord Wharton’s Jelly (WJSC) in Younger Women Giving Birth Naturally

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