Maternal physiology and blastocyst morphology are correlated with an inherent difference in peri-implantation human embryo development

Logsdon, Deirdre M.; Grimm, Courtney K.; West, Rachel C.; Engelhorn, Heidi J.; Kile, Rebecca; Reed, Laura; Swain, Jason E.; Katz‐Jaffe, Mandy G.; Schoolcraft, William B.; Krisher, Rebecca L.; Yuan, Ye

doi:10.1016/j.fertnstert.2022.02.018

Cited by 4 publications

(2 citation statements)

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“…To minimize platform differences, in this study, we sequenced 5 day post fertilization (d.p.f.) human blastocysts (n=50) with widely accepted good quality grades above 2/3 and internal historical live birth data above 65% (Logsdon et al, 2022) side-by-side with blastoids (n=100) using the Chromium platform. To further reduce batch variation, we prepared one of the libraries on cells pooled from both human blastocysts and blastoids, and deconvoluted each cell’s origin by using sequenced genotypes (Figures S3A-S3D).…”

Section: Resultsmentioning

confidence: 99%

Section: Single Cell Transcriptomes Of Human Blastocysts and Blastoidsmentioning

confidence: 99%

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Large scale production of human blastoids amenable to modeling blastocyst development and maternal-fetal crosstalk

Ezashi

Wei

et al. 2022

Preprint

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SUMMARYRecent advances in human blastoids generated from naïve pluripotent stem cells have opened a new avenue for modelling early human development and implantation. Despite the success, however, existing protocols have several limitations, e.g., the use of custom-built microwell arrays impedes wide adoption by the research community, and mass production of human blastoids is hampered by low-output or low-efficiency methods. To address these issues, here we developed an optimized protocol based on commercially available microwell plates, which enabled efficient generation of high-fidelity human blastoids at a large scale. Leveraging on the improved protocol, we identified MAPK. PI3K/AKT and mTOR signaling pathways were activated in both blastoids and blastocyst, and discovered endometrial stromal effects in promoting trophoblast cell survival, proliferation and syncytialization during extended co-culture with blastoids. Our optimized protocol will facilitate broader use of human blastoids as an accessible, perturbable, scalable, tractable, and ethical model for human blastocysts.

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Section: Resultsmentioning

confidence: 99%

Section: Single Cell Transcriptomes Of Human Blastocysts and Blastoidsmentioning

confidence: 99%