Maternal peripheral T helper 1-type and T helper 2-type immunity in women during the first trimester of twin pregnancy

Suzuki, Shunji; Okudaira, Sayaka

doi:10.1007/s00404-003-0549-y

Cited by 10 publications

(13 citation statements)

References 18 publications

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“…We hypothesize that, in addition to a generalized status of immunological deficiency or insufficiency, the copresence of twins in utero may induce a deeper immune tolerance that involves both fetuses and the mother in order to avoid the potentially harmful immune reaction between the three. Our hypothesis is in accordance with previous studies that documented higher levels of Th2-cytokines in the blood of mothers carrying twins compared with singleton pregnancies [24] underlining the more profound Th1-Th2 shift that occurs in twin pregnancies. Moreover, a recent study demonstrated that both dizygotic twins and their mothers are more prone to infection than monozygotic twins, singletons, and their mothers [25].…”

Section: Discussionsupporting

confidence: 93%

Altered Intracellular ATP Production by Activated CD4+ T-Cells in Very Preterm Infants

Aquilano

Capretti

Nanni

et al. 2016

Journal of Immunology Research

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Background. The neonatal immune system is not fully developed at birth; newborns have adequate lymphocytes counts but these cells lack function. Objective. To assess the activity of T-cells and the influence of the main perinatal factors in very preterm infants (birth weight < 1500 g). Design. Blood samples from 59 preterm infants (21/59 were dizygotic twins) were collected at birth and at 30 days of life to measure CD4+ T-cell activity using the ImmuKnow™ assay. Fifteen healthy adults were included as a control group. Results. CD4+ T-cell activity was lower in VLBW infants compared with adults (p < 0.001). Twins showed lower immune activity compared to singletons (p = 0.005). Infants born vaginally showed higher CD4+ T-cell activity compared to those born by C-section (p = 0.031); infants born after prolonged Premature Rupture of Membranes (pPROM) showed higher CD4+ T-cell activity at birth (p = 0.002) compared to infants born without pPROM. Low CD4+ T-cell activity at birth is associated with necrotizing enterocolitis (NEC) in the first week of life (p = 0.049). Conclusions. Preterm infants show a lack in CD4+ T-cell activity at birth. Perinatal factors such as intrauterine inflammation, mode of delivery, and zygosity can influence the adaptive immune activation capacity at birth and can contribute to exposing these infants to serious complications such as NEC.

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Section: Discussionsupporting

confidence: 93%

Altered Intracellular ATP Production by Activated CD4+ T-Cells in Very Preterm Infants

Aquilano

Capretti

Nanni

et al. 2016

Journal of Immunology Research

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“…Normal human pregnancies are distinguished by a Th2 bias (Poole and Claman, 2004;Kidd, 2003;Sacks et al, 2003;Suzuki and Okudaira, 2004;Shimaoka et al, 2000;Lim et al, 2000;Piccinni et al, 2000;Saito et al, 1999). The shift from Th1 cytokine production to Th2 cytokine production protects the semi-allogeneic fetus from rejection by the maternal immune system, but as gestation progresses a shift from a predominate Th2 response to a Th1 response occurs (Raghupathy et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Validation and comparison of luminex multiplex cytokine analysis kits with ELISA: Determinations of a panel of nine cytokines in clinical sample culture supernatants

duPont¹,

Wang

Wadhwa

et al. 2005

Journal of Reproductive Immunology

272

181

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Problem: Analyses of the expression pattern of multiple cytokines are frequently required for characterization of the status of the immune system as it pertains to Th type bias and intrinsic levels of inflammation. Classically, analysis of cytokine expression patterns has been performed by enzymelinked immunosorbent assays (ELISA) for each separate analyte. A new technology, Luminex MAP ® , facilitates the simultaneous evaluation of multiple immune mediators with advantages of higher throughput, smaller sample volume, and lower cost. Validation of this technology has been limited to small sample sets, limited use of clinical study specimens, and use of non-commercial reagents. * Corresponding author. Methods: Ninety-six specimens from women over the course of their respective pregnancies were evaluated for cytokine concentrations using commercially available ELISA kits and commercially available Luminex MAP ® kits according to the manufacturers' directions. Correlations between data sets were evaluated using Pearson's correlation coefficient (r).Results: Excellent correlations were demonstrated for IL-1␤, IL-4, IL-5, IL-6, IL-10, IFN ␥, and TNF ␣, in contrast to IL-12 p70 and IL-13. Conclusions: Luminex multiplex technology has distinct advantages and is a valid alternative method to ELISA for the evaluation of the majority of cytokines tested and for the characterization of immune system status.

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“…. The median (range) gestational age at sample collection was 22(18)(19)(20)(21)(22)(23)(24)(25)(26)(27) weeks; a2V concentrations were positive correlated with gestational age at sample collection (P = 0.023, Spearman r = 0.237) but not with maternal age. Thirtyseven pregnancies were conceived with no intervention or following intrauterine insemination or ovarian stimulation, and 56 were conceived by in vitro fertilization-embryo transfer.…”

mentioning

confidence: 91%

“…An elevated level of the Th2 subclass of T lymphocytes and a decreased Th1-Th2 T lymphocyte ratio has been observed in mothers with twins compared to those with singleton pregnancies. 17,18 In the present study, we evaluated if a2V levels in peripheral blood mononuclear cells (PBMCs) were different depending on fetal sex, and if this was reflected in associations with birthweight and gestational age at delivery.…”

Section: Introductionmentioning

confidence: 99%

Sex‐specific influence of the vacuolar adenosine triphosphatase a2 isoform on outcome in twin pregnancies

Sisti

Tommaso

Paccosi

et al. 2018

American J Rep Immunol

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Problem:The influence of fetal sex on immune responses in multifetal pregnancies remains incompletely elucidated. The a2 isoform of vacuolar adenosine triphosphatase (a2V) is expressed on the cell membrane of maternal lymphoid cells and contributes to down-regulation of pro-inflammatory immune responses during gestation.The association between fetal sex and a2V expression on peripheral blood mononuclear cells (PBMCs) from mothers with twin gestations was assessed. Method of study:Patients in this prospective study were 93 women with twin pregnancies in their mid-second or early third trimester-27 with two male, 30 with two female and 36 with one male and one female fetus. PBMCs were isolated and a2V was measured by ELISA in cell lysates. Demographic and clinical data were subsequently obtained and correlations between a2V and fetal sex, birthweight and pregnancy outcome were assessed by the Mann-Whitney and Spearman rank correlation tests. Results:The mean a2V level was highest when both fetuses were male (2.0 ng/mL) and lowest when both were female (1.5 ng/mL; P = 0.0184). Only when both fetuses were female did the a2V concentration negatively correlate with birthweight of the 1st (P = 0.0011) and 2nd (P = 0.0044) born fetus and with gestational age at delivery (P = 0.0018). There were no associations between a2V and these outcomes in male only or mixed twin pregnancies. Conclusion:We conclude that the a2V-mediated regulation of maternal immunity during twin pregnancies is influenced by fetal sex. K E Y W O R D S a2v, female, fetal outcome, twins How to cite this article: Sisti G, Di Tommaso M, Paccosi S, et al. Sex-specific influence of the vacuolar adenosine triphosphatase a2 isoform on outcome in twin pregnancies. Am

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Maternal peripheral T helper 1-type and T helper 2-type immunity in women during the first trimester of twin pregnancy

Abstract: Our findings show marked predominance of Th2 type cytokines occurring in twin pregnancies is related to the increase in trophoblasts during the first trimester.

Cited by 10 publications

References 18 publications

Altered Intracellular ATP Production by Activated CD4+ T-Cells in Very Preterm Infants

Altered Intracellular ATP Production by Activated CD4+ T-Cells in Very Preterm Infants

Validation and comparison of luminex multiplex cytokine analysis kits with ELISA: Determinations of a panel of nine cytokines in clinical sample culture supernatants

Sex‐specific influence of the vacuolar adenosine triphosphatase a2 isoform on outcome in twin pregnancies

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