Maternal overweight increased sensitivity of mouse preimplantation embryos to oxidative stress in vitro

Šefčíková, Zuzana; Babeľová, Janka; Kovaříková, Veronika; Koppel, Juraj; Fabian, Denis

doi:10.1016/j.reprotox.2021.08.006

Cited by 1 publication

(2 citation statements)

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“…Moreover, significantly higher rates of meiotic spindle and mitochondrial defects were found in oocytes of "diet reversal mice" exhibiting normalization of weight, glucose utilization, and cholesterol levels eight weeks after switching from a highfat diet to regular chow [9]. Finally, matured oocytes recovered from obese mice developed in an intergenerational model showed significantly lowered deposits of neutral lipids in the cytoplasm [19], higher DNA methylation in the nuclear area [16], and significantly lower expression of Glutathione Peroxidase 8 [37].…”

Section: Fertilization Ratementioning

confidence: 99%

“…As shown in our previous studies, blastocysts recovered from obese mice displayed significantly elevated incidence of apoptotic cell death [11], altered gene expression (higher BAX/BCL2L2 ratio, higher expression of insulin receptor [16], and higher expression of GLUT4, an insulin-responsive glucose transporter [15]), and development of insulin resistance [16]. Furthermore, the origin of embryos (from obese vs. control dams) significantly altered their overall metabolic activity in vitro, i.e., their metabolomic profile assessed by means of Raman spectroscopy [20], their response to leptin and insulin during development in vitro [15,16], and their sensitivity to oxidative stress during development in vitro [37]. Similarly, in human in vitro developed blastocysts, fewer cells in the trophectoderm and poor glucose uptake were demonstrated in a group of obese women [43].…”

Section: Developmental Ratementioning

confidence: 99%

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Overweight and Fertility: What We Can Learn from an Intergenerational Mouse Obesity Model

Fabian

Kubandová-Babeľová

Kšiňanová

et al. 2022

IJERPH

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The aim of this study was to evaluate the effects of being overweight on the ability to conceive, fertilization rate, and in vivo development of embryos in regularly cycling, spontaneously ovulating, and naturally mated female mice. The study was based on statistical analysis of data collected during 14 experiments with identical design, performed on 319 control and 327 obese mice, developed in an intergenerational model of obesity induction which eliminates the impact of aging and high-fat feeding. Six-week-old mice with a vaginal sperm plug were slaughtered on embryonic days 2, 3, or 4, and the flushed contents of the oviducts and uteri were assessed by stereomicroscopy. The results showed no association between being overweight and the proportion of ovulating or fertilized females. On the other hand, a strong association was found between being overweight and ovulation yield. On embryonic day 2, significantly higher numbers of eggs were recovered from the oviducts of fertilized obese mice. Maternal overweight status was also associated with higher developmental capacities of preimplantation embryos. In conclusion, contrary to studies based on the high-fat-diet model, in female mice fed regular chow, being overweight was associated with an increased ovulation quota and higher developmental rate of fertilized oocytes. Being overweight did not impact ability to conceive. On the other hand, as documented in our previous studies, the quality of oocytes and blastocysts recovered from overweight mice developed in an intergenerational model of obesity was low.

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