Maternal obesity accelerates rat offspring metabolic ageing in a sex‐dependent manner

Ogunsola

2020

Preprint

To test the hypothesis that maternal undernutrition exerts a greater effect on offspring metabolic function compared to maternal overnutrition, female rats (n=10 per group) were subjected to a high calorie diet or 50 % global nutrient restriction relative to control rats on standard rat chow, for 8 weeks prior to pregnancy and during pregnancy. Birth weight was determined on the day dams were found with pups. At 3 months of age, offspring’s fasting blood glucose, serum insulin and triglyceride levels as well as glucose tolerance and insulin sensitivity were determined. A 50 % calorie restriction caused a significant weight loss in the under-nourished dams but those on high calorie diet had similar body weight as control rats. Maternal overnutrition and undernutrition significantly lowered birth weight, indicating intra-uterine growth restriction in these animals. Fasting blood glucose was significantly higher in female offspring of over-nourished dams, but neither maternal overnutrition nor undernutrition affected offspring’s glucose tolerance. Male offspring of dams exposed to maternal overnutrition or undernutrition had a significantly higher insulin level compared to control, whereas female offspring were unaffected. The development of hyperinsulinaemia in male offspring of undernourished dams was accompanied by reduced insulin sensitivity. This study demonstrates that early-life exposure to two extreme ends of the nutritional plane is associated with similar birth weight outcome but different metabolic phenotype in adulthood. Evidence of insulin resistance only in male offspring of under-nourished dams indicates differences in sex-specific metabolic effect of maternal undernutrition compared to overnutrition.

Section: Discussionmentioning

confidence: 65%

Differential impact of maternal overnutrition and undernutrition on offspring glucose homeostasis

Ogunsola

2020

Preprint

“…This work has been carried out using separately male and female, but other papers support these sex- and age-dependent results. In fact, it has been demonstrated that age and sex differently impact on cholesterol metabolism in LDL -/- mice [ 23 ] and in human beings [ 24 ].…”

Section: Sex- and Age-dependent Differences Of Mva Pathway In The mentioning

confidence: 99%

Impact of Sex and Age on the Mevalonate Pathway in the Brain: A Focus on Effects Induced by Maternal Exposure to Exogenous Compounds

2020

The mevalonate pathway produces cholesterol and other compounds crucial for numerous cellular processes. It is well known that age and sex modulate this pathway in the liver. Recently, similar effects were also noted in different brain areas, suggesting that alterations of the mevalonate pathway are at the root of marked sex-specific disparities in some neurodevelopmental disorders related to disturbed cholesterol homeostasis. Here, we show how the mevalonate pathway is modulated in a sex-, age- and region-specific manner, and how maternal exposure to exogenous compounds can disturb the regulation of this pathway in the brain, possibly inducing functional alterations.

“…The high-energy obesogenic diet was produced in the specialized dietary facility of the INCMNSZ. 20 Thus, each F0 group contained only one female from any litter and F0 females in different groups, but not within groups, were sisters, providing homogeneity in F0 mothers' own developmental programming and genetics.…”

Section: Animal Care Diets and Experimental Designmentioning

confidence: 99%

Impact of Leuconostoc SD23 intake in obese pregnant rats: benefits for maternal metabolism

Castro‐Rodríguez

Juárez-Pilares

Cano-Cano

et al. 2020

J Dev Orig Health Dis

Self Cite

Maternal obesity (MO) during pregnancy and lactation leads to maternal and offspring metabolic dysfunction. Recent research has suggested that probiotics might be a novel approach to counteract these unwanted MO effects. The aim of this research was to analyze the impact of Leuconostoc SD23, a probiotic isolated from aguamiel (traditional Mexican drink), on MO metabolism in rats at the end of lactation (21 days). From weaning through lactation, control female Wistar rats (C) ate chow (5% fat) or high-energy obesogenic diet (MO; 25% fat). Half the C and MO mothers received a daily dose (1 × 1010 CFU/ml) of probiotic orally, control with probiotic (CP) and MO with probiotic (MOP), 1 month before mating and through pregnancy and lactation. Histological analyses of the liver, white adipose tissue and small intestine, body composition, glucose, insulin, triglycerides, and leptin were determined in mothers at the end of lactation. Maternal weight during pregnancy was greater in MO than C mothers, but similar at the end of lactation. Probiotic intervention had no effect on maternal weight. However, at the end of lactation, percentage of body fat was higher in MO than C, CP, and MOP. Serum glucose, homeostasis model assessment of insulin resistance, and triglycerides were higher in MO versus C, CP, and MOP. MO small intestine villus height was higher versus MOP, C, and CP. Leuconostoc SD23 did not present adverse effects in C. Conclusions: maternal administration of Leuconostoc SD23 has beneficial effects on maternal metabolism, which holds possibilities for preventing adverse offspring metabolic programming.