Maternal/newborn genotype contribution of the renin–angiotensin system (Met235Thr, Thr174Met, I/D-ACE, A2350G-ACE, A1166C-AT2R1, C3123A- AT2R2, ⁸³A/G-REN) to the risk of pre-eclampsia: a Romanian study

Abstract

Introduction:We evaluated the association of the mutated genotypes Met235Thr-AGT, Thr174Met-AGT, I/D-ACE, A2350G-ACE, A1166C-AT2R1, C3123A-AT2R2, 83 A/G-REN with the risk and outcome of pre-eclampsia; we also investigated whether genes in newborns increase maternal risk of pre-eclampsia. Materials and methods: Thirty-six pairs of pre-eclamptic women and their newborns were genotyped, along with 71 pairs of controls (mothers/newborns) using PCR-RFLP analysis. Results: The Thr235/Thr235 (OR 3.44, p = 0.01), DD (OR 2.66, p = 0.039), CC1166 (OR 5.56, p = 0.04), AA3123 (OR 3.77, p = 0.03) and GG 83 (OR 8.32, p = 0.006) genotypes are significantly associated with pre-eclampsia. Women with pre-eclampsia positive for Met235Thr (34.64 ± 3.92 weeks vs. 38 ± 2 weeks), Thr174Met (32.58 ± 3.92 weeks vs. 36.38 ± 3.25 weeks), I/D (34.47 ± 3.67 weeks vs. 38.33 ± 3.5 weeks) delivered at a significant lower gestational age compared with pre-eclamptic women with a normal genotype. Newborns from women with pre-eclampsia positive for Thr174Met (2190 ± 820.21 g vs. 2702.08 ± 967.23 g), I/D (2399.33 ± 938.38 g vs. 3191.66 ± 684.40 g) had a significant lower birth weight compared with newborns from women with normal pregnancies. When both the mother and the newborn were positive for Met235Thr,

The Gene Variants of Maternal/Fetal Renin-Angiotensin System in Preeclampsia: A Hybrid Case-Parent/Mother-Control Study

“…A previous Romanian case-control study included 36 mother/newborn pairs with PE complications and 71 controls. Their findings on fetal ACE G2350A, AGT 174M, and ACE I/D were in accordance with our results 21 . The discrepancy in the effect of fetal AGT M235T, which was positive in the current study but negative in the Romanian study, may partly arise from the different study designs, besides the different genetic background and sample size.…”

“…Many previous studies have investigated the association between the five maternal genotypes and PE, but the conclusions were weak and inconsistent 21, 35, 48, 49 . The maternal ACE I/D, AGT M235T, AGT T174M, and AT1R A1166C genotypes were not associated with PE in our study, which is consistent with the results of the recently published meta-analyses 50–52 .…”

Associations of ACE I/D and AGT M235T Gene Polymorphisms with the Gestational Hypertension and the Fetal Growth

“…In one study the Thr235/Thr235 of AGT and DD of the ACE genotypes were significantly associated with preeclampsia, 25 which stays in the disagreement with the results our study. The results of this study are consistent with the different study on the Polish population, 26 where there was no difference in allele frequency I/D ACE gene between the control group and the group with gestational hypertension or research on the Korean population 27 which did not confirm the role of the I/D polymorphism in preeclampsia.…”

Association of maternalAGTR1polymorphisms and preeclampsia: a systematic review and meta-analysis

“…The full-text of the remaining 12 articles was retrieved for review and three additional articles were excluded for shared subjects. Multiple publications were discovered for two data sets: a United Kingdom (UK) population [18–20] and a Romanian population [21, 22]. For the UK population, the study with the largest number of subjects was retained [20].…”