Maternal neutrophil apoptosis in normal pregnancy, preeclampsia, and normotensive intrauterine growth restriction

Dadelszen, Peter von; Watson, R.W.G.; Noorwali, Fatma; Marshall, John C.; Parodo, Jean; Farine, Dan; Lye, S. J.; Ritchie, Jackie; Rotstein, Ori D.

doi:10.1016/s0002-9378(99)70570-3

Cited by 103 publications

(95 citation statements)

References 16 publications

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“…These data reinforce the conjecture that both the origins (i.e amount of circulating placental debris) and maternal inflammatory response of early-onset pre-eclampsia may explain the differential maternal response and risks of early-onset versus both late-onset pre-eclampsia and/or normotensive IUGR. [22][23][24] We chose to study neutrophils because they play a key role in innate immune defence 25 and there is evidence of their role in pre-eclampsia. 3,22,26,27 In addition, neutrophils express extracellular receptors, TLR2 and TLR4, and the intracellular cryopyrin inflammasome, which are crucial to inflammatory cytokine production.…”

Section: Discussionmentioning

confidence: 99%

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Toll‐like receptors 2 and 4 and the cryopyrin inflammasome in normal pregnancy and pre‐eclampsia

Xie¹,

Hu²,

Turvey

et al. 2009

BJOG

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Objective Pre-eclampsia involves a maternal inflammatory response that differs from both normal pregnancy and normotensive intrauterine growth restriction (IUGR). Our objective was to examine neutrophil Toll-like receptor (TLR), cryopyrin, nuclear factor-jB (NF-jB) subunit and interleukin-1b (IL-1b), and inflammatory cytokine profiles in women with preeclampsia or normotensive IUGR, as well as in normal pregnancy and non-pregnancy controls.Design and method A case-control study was performed. We examined the messenger RNA (mRNA) and protein expressions of TLR4 and TLR2, mRNA levels of cryopyrin, IL-1b, NF-jB subunits p50 and p65, as well as maternal serum inflammatory cytokine profiles (IL-2, IL-6, tumour necrosis factor-a [TNF-a], interferon-c [IFN-c] and IL-10) in women with and without pre-eclampsia using real-time reverse transcription polymerase chain reactions, flow cytometry and multiplex immunoassays.Setting A single tertiary maternity hospital in Vancouver, Canada.Population Women with early-onset pre-eclampsia (<34 weeks of gestation, n = 25), women with late-onset pre-eclampsia ( ‡34 +0 weeks of gestation, n = 25), women with normotensive IUGR (n = 25), women with normal pregnancy (n = 75) and non-pregnancy (n = 25) controls.Results Women with pre-eclampsia (as a single combined group of early-and late-onset, and particularly in women with earlyonset pre-eclampsia) had increased TLR2 and TLR4 mRNA and protein expressions elevated cryopyrin, NF-jB subunit, and IL-1b mRNA expression, and TNF-a:IL-10 and IL-6:IL-10 ratios compared with other groups.Conclusions These data suggest that TLRs and cryopyrin may modulate the innate immune response of the maternal syndrome of pre-eclampsia, and might also trigger the differential inflammatory response existing between early onset pre-eclampsia and normotensive IUGR.

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Section: Discussionmentioning

confidence: 99%

“…The effects of pregnancy per se on these aspects of innate immune activation were considered important. 22 All pregnancies were singleton gestations.…”

Section: Methodsmentioning

confidence: 99%

Toll‐like receptors 2 and 4 and the cryopyrin inflammasome in normal pregnancy and pre‐eclampsia

Xie¹,

Hu²,

Turvey

et al. 2009

BJOG

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“…Apoptosis, or programmed cell death, has been implicated in the maintenance of tissue homeostasis [19][20][21][22] and the pathophysiology of pre-eclampsia [23][24][25][26][27][28][29][30] . The Fas/Fas ligand (Fas/FasL) system, also known as the death factor system, is one of the main apoptotic pathways.…”

Section: Introductionmentioning

confidence: 99%

Maternal serum of women with pre-eclampsia reduces trophoblast cell viability: evidence for an increased sensitivity to Fas-mediated apoptosis

Neale

Demasio

Illuzi

et al. 2003

The Journal of Maternal-Fetal & Neonatal Medicine

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“…Moreover, apoptosis of PMN from women at the end of pregnancy is extensively delayed [37]. Evaluation of the administration of 17β-estradiol and/or progesterone to human PMN confirmed this suggestion.…”

Section: Introductionmentioning

confidence: 67%

Influence of sex steroids on the viability and CD11b, CD18 and CD47 expression of blood neutrophils from dairy cows in the last month of gestation

et al. 2006

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-In the period around parturition, cows experience an increased susceptibility for the development of Escherichia coli mastitis. This increased susceptibility has been correlated with a decreased functionality of neutrophils. In the current study, it is suggested that the decreased neutrophil functionality may be induced by the extensive alterations in sex steroid levels occurring around parturition. It was first hypothesized that 17β-estradiol and progesterone influence the viability, apoptosis and necrosis of blood neutrophils from cows in their last month of gestation. Subsequently, it was hypothesized that 17β-estradiol modulates the expression of CD11b, CD18 or CD47 thereby explaining its influence on the migration of bovine neutrophils. Neither 17β-estradiol nor progesterone significantly influenced viability, apoptosis or necrosis in spontaneous apoptosis conditions. However, when apoptosis was induced with TNF-α and gliotoxin, progesterone exerted a survival effect (P < 0.05). In addition, 17β-estradiol treatment of bovine blood neutrophils significantly decreased the expression of CD47 (P < 0.05) but not of CD11b or CD18. It can be concluded that 17β-estradiol and progesterone do not affect spontaneous apoptosis of bovine blood neutrophils while a survival effect was observed for progesterone on induced neutrophils apoptosis. Moreover, our results concerning the influence of 17β-estradiol on the CD11b, CD18 and CD47 expression extend previous demonstrations of the suppressive effect of 17β-estradiol on neutrophils migration and indicate that the altered expression of CD47 may contribute to this phenomenon. sex steroid / β 2 -integrin / CD47 / viability / polymorphonuclear neutrophil leukocyte

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Maternal neutrophil apoptosis in normal pregnancy, preeclampsia, and normotensive intrauterine growth restriction

Cited by 103 publications

References 16 publications

Toll‐like receptors 2 and 4 and the cryopyrin inflammasome in normal pregnancy and pre‐eclampsia

Toll‐like receptors 2 and 4 and the cryopyrin inflammasome in normal pregnancy and pre‐eclampsia

Maternal serum of women with pre-eclampsia reduces trophoblast cell viability: evidence for an increased sensitivity to Fas-mediated apoptosis

Influence of sex steroids on the viability and CD11b, CD18 and CD47 expression of blood neutrophils from dairy cows in the last month of gestation

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