Maternal micronutrients can modify colonic mucosal microbiota maturation in murine offspring

Nagy‐Szakal, Dorottya; Ross, Matthew C.; Dowd, Scot E.; Mir, Sabina; Schaible, Tiffany D.; Petrosino, Joseph F.; Kellermayer, Richárd

doi:10.4161/gmic.20697

Cited by 31 publications

(34 citation statements)

References 37 publications

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“…independent from perinatal colonization) [33]. The findings of this work conclusively show that prenatal MD supplementation is sufficient to modulate gut microbiome composition independently from postnatal maternal exposures.…”

Section: Discussionmentioning

confidence: 55%

“…during a sterile stage of development) transmitting microbiome composition changes into postnatal development. Our previous investigations indicated that MD supplementation may modify host maturation towards maintaining a colitogenic microbiome independently from the inherited dam microbiomes [33]. To confirm these findings, we examined the effects of prenatal MD supplementation on colonic mucosal and luminal (cecal content) microbiomes in the MD-C and C-C groups.…”

Section: Resultsmentioning

confidence: 82%

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Prenatal Methyl-Donor Supplementation Augments Colitis in Young Adult Mice

Mir

Nagy‐Szakal

Dowd³

et al. 2013

PLoS ONE

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Inflammatory bowel diseases (IBD) have become highly prevalent in developed countries. Environmentally triggered exaggerated immune responses against the intestinal microbiome are thought to mediate the disorders. The potential dietary origins of the disease group have been implicated. However, the effects of environmental influences on prenatal developmental programming in respect to orchestrating postnatal microbiome composition and predilection towards mammalian colitis have not been examined. We tested how transient prenatal exposure to methyl donor micronutrient (MD) supplemented diets may impact predilection towards IBD in a murine dextran sulfate sodium (DSS) colitis model. Prenatal MD supplementation was sufficient to modulate colonic mucosal Ppara expression (3.2 fold increase; p=0.022) and worsen DSS colitis in young adulthood. The prenatal dietary exposure shifted the postnatal colonic mucosal and cecal content microbiomes. Transfer of the gut microbiome from prenatally MD supplemented young adult animals into germ free mice resulted in increased colitis susceptibility in the recipients compared to controls. Therefore, the prenatal dietary intervention induced the postnatal nurturing of a colitogenic microbiome. Our results show that prenatal nutritional programming can modulate the mammalian host to harbor a colitogenic microbiome. These findings may be relevant for the nutritional developmental origins of IBD.

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Section: Discussionmentioning

confidence: 55%

Section: Resultsmentioning

confidence: 82%

Prenatal Methyl-Donor Supplementation Augments Colitis in Young Adult Mice

Mir

Nagy‐Szakal

Dowd³

et al. 2013

PLoS ONE

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“…Bacterial tag-encoded FLX amplicon pyrosequencing (bTE-FAP) was done as described previously [6,[19][20][21][22][23], but with the new technique of bacterial tag-encoded FLX titanium amplicon pyrosequencing (bTETAP). This technique is based on bTEFAP but utilizes titanium-containing reagents and titanium-based procedures, with a one-step PCR, a mixture of hot start and hot start high-fidelity Taq polymerases, and amplicons originating from the 28F 5¢GAGTTTGATCNTGGCTCAG to 519R 5¢GTNTTACNGCGGCKGCTG regions of the rRNA of Escherichia coli, and provides results for the V1-V3 regions of the 16S ribosome.…”

Section: Pyrosequencingmentioning

confidence: 99%

“…Following pyrosequencing of the 16S rRNA in the patient specimens, all unsuccessful sequence readings, low-quality sequence ends, and tags were removed, and any non-bacterial ribosome sequences and chimeras were removed from the specimens with the use of software described previously [6,[19][20][21][22]. Sequences shorter than 350 bp according to the bTEFAP method were excluded.…”

Section: Analysis Of Bacterial Diversity Datamentioning

confidence: 99%

Examination with Next-Generation Sequencing Technology of the Bacterial Microbiota in Bronchoalveolar Lavage Samples after Traumatic Injury

Huebinger

Liu

Dowd³

et al. 2013

Surgical Infections

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Background: We examined the microbiota of bronchoalveolar lavage (BAL) samples with next-generation sequencing (NGS) technology to determine whether its results correlate with those of standard culture methods or affect patient outcome or both. Methods: We collected BAL samples in the surgical intensive care unit (SICU) as part of the standard of care for intubated individuals who had a Clinical Pulmonary Infection Score (CPIS) ‡ 6 points. A portion of the BAL fluid was sequenced for the 16S region of ribosomal deoxyribonucleic acid (rDNA) with the Roche 454 FLX Titanium sequencer. Sequences were analyzed through a data-analysis pipeline to identify the appropriate taxonomic designation (*species) of each 16s sequence. The bacterial microbiota of each BAL sample was compared with the bacteria identified in the sample through standard culture methods. Correlations between the taxonomic diversity of the microbiota and clinical outcome were examined through linear regression and Pearson correlation. Results: Bronchoalveolar lavage samples from 12 individuals in the SICU who had a CPIS ‡ 6 points were examined through 454 pyrosequencing. The number of phylotypes (*species) in the samples ranged from 15 to 129. The number of phyla in the BAL samples ranged from 3 to 14. There was little correlation between the bacteria identified by NGS and those identified with standard culture methods. The same predominant bacterial strain was identified by both culture and sequencing in only a single sample. The correlation between patient days on a ventilator and the number of species in BAL samples was significant (r = 0.7435, p = 0.0056; r 2 = 0.5528). Conclusions: Increasing diversity of the bacterial microbiota in BAL samples correlates with the duration of mechanical ventilation. Bacteria identified through standard culture methods were not well correlated with the findings of NGS.

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“…Developmental dietary intervention induced alteration of normal colonic mucosa-associated microbiota shifts may have contributed to the observed increase in colitis susceptibility in the MD supplemented offspring. 173 Thus, prenatal nutritional programming can modulate the mammalian host to harbor a colitogenic microbiome. 174 These studies provide important insights into microbespecific immunity through epigenetic regulation.…”

Section: Epigenetic Control and Intestinal Microbiotamentioning

confidence: 99%

Linking Immunity, Epigenetics, and Cancer in Inflammatory Bowel Disease

Däbritz

Menheniott

2014

Inflammatory Bowel Diseases

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Most of what is known about the pathogenesis of inflammatory bowel disease (IBD) pertains to complex interplay between host genetics, immunity, and environmental factors. Epigenetic modifications play pivotal roles in intestinal immunity and mucosal homeostasis as well as mediating gene-environment interactions. In this article, we provide a historical account of epigenetic research either directly related or pertinent to the pathogenesis and management of IBD. We further collate emerging evidence supporting roles for epigenetic mechanisms in relevant aspects of IBD biology, including deregulated immunity, host-pathogen recognition and mucosal integrity. Finally, we highlight key epigenetic mechanisms that link chronic inflammation to specific IBD comorbidities, including colitis-associated cancer and discuss their potential utility as novel biomarkers or pharmacologic targets in IBD therapy.

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Maternal micronutrients can modify colonic mucosal microbiota maturation in murine offspring

Cited by 31 publications

References 37 publications

Prenatal Methyl-Donor Supplementation Augments Colitis in Young Adult Mice

Prenatal Methyl-Donor Supplementation Augments Colitis in Young Adult Mice

Examination with Next-Generation Sequencing Technology of the Bacterial Microbiota in Bronchoalveolar Lavage Samples after Traumatic Injury

Linking Immunity, Epigenetics, and Cancer in Inflammatory Bowel Disease

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