Maternal-fetal interfaces transcriptome changes associated with placental insufficiency and a novel gene therapy intervention

Abstract

The etiology of fetal growth restriction (FGR) is multifactorial, although many cases often involve placental insufficiency. Placental insufficiency is associated with inadequate trophoblast invasion resulting in high resistance to blood flow, decreased availability of nutrients, and increased hypoxia. We have developed a non-viral, polymer-based nanoparticle that facilitates delivery and transient gene expression ofhuman insulin-like 1 growth factor(hIGF1) in placental trophoblast for the treatment of placenta insufficiency and FGR. Using the established guinea pig maternal nutrient restriction (MNR) model of placental insufficiency and FGR, the aim of the study was to identify novel pathways in the sub-placenta/decidua that provide insight into the underlying mechanism driving placental insufficiency, and may be corrected withhIGF1nanoparticle treatment. Pregnant guinea pigs underwent ultrasound-guided sham orhIGF1nanoparticle treatment at mid-pregnancy, and sub-placenta/decidua tissue was collected 5 days later. Transcriptome analysis was performed using RNA Sequencing on the Illumina platform. The MNR sub-placenta/decidua demonstrated fewer maternal spiral arteries lined by trophoblast, shallower trophoblast invasion and downregulation of genelists involved in the regulation of cell migration.hIGF1nanoparticle treatment resulted in marked changes to transporter activity in the MNR +hIGF1sub-placenta/decidua when compared to sham MNR. Under normal growth conditions however,hIGF1nanoparticle treatment decreased genelists enriched for kinase signaling pathways and increased genelists enriched for proteolysis indicative of homeostasis. Overall, this study identified changes to the sub-placenta/decidua transcriptome that likely result in inadequate trophoblast invasion and increases our understanding of pathways thathIGF1nanoparticle treatment acts on in order to restore or maintain appropriate placenta function.