Maternal Eomesodermin regulates zygotic nodal gene expression for mesendoderm induction in zebrafish embryos

Xu, Pengfei; Zhu, Gaoyang; Wang, Yixia; Sun, Jiawei; Liu, Xingfeng; Chen, Ye-Guang; Meng, Anming

doi:10.1093/jmcb/mju028

Cited by 30 publications

(36 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In zebrafish, perturbation analysis reveals that Eomesa is required for the activation of sox17 , as well as the two nodal ligands squint and cyclops [45]. ChIP analyses confirm that Eomesa physically binds to the squint locus [46,47]. Eomesa also binds regulatory regions near sox17 , and additional binding was observed for mixl1 , foxa , foxa3 , vent , and gsc [46].…”

Section: Maternal Factors and The Initiation Of The Networkmentioning

confidence: 99%

A gene regulatory program controlling early Xenopus mesendoderm formation: Network conservation and motifs

Charney

Paraiso

Blitz

et al. 2017

Seminars in Cell & Developmental Biology

View full text Add to dashboard Cite

Germ layer formation is among the earliest differentiation events in metazoan embryos. In triploblasts, three germ layers are formed, among which the endoderm gives rise to the epithelial lining of the gut tube and associated organs including the liver, pancreas and lungs. In frogs (Xenopus), where early germ layer formation has been studied intensively, the process of endoderm specification involves the interplay of dozens of transcription factors. Here, we review the interactions between these factors, summarized in a transcriptional gene regulatory network (GRN). We highlight regulatory connections conserved between Xenopus, zebrafish, mouse, and human endodermal lineages. Especially prominent is the conserved role and regulatory targets of the Nodal signaling pathway and the T-box transcription factors, Vegt and Eomes. Additionally, we highlight some network topologies and motifs, and speculate on their possible roles in development.

show abstract

Section: Maternal Factors and The Initiation Of The Networkmentioning

confidence: 99%

A gene regulatory program controlling early Xenopus mesendoderm formation: Network conservation and motifs

Charney

Paraiso

Blitz

et al. 2017

Seminars in Cell & Developmental Biology

View full text Add to dashboard Cite

show abstract

“…Whole-mount immunofluorescence was carried out according to a standard protocol, as previously described 62…”

Section: Whole-mount Immunofluorescence Of Zebrafish Embryosmentioning

confidence: 99%

Critical role for cold shock protein YB-1 in cytokinesis

Mehta

Algie

Al-Jabri

et al. 2020

Preprint

View full text Add to dashboard Cite

Y-box-binding protein-1, YB-1, is essential for cell division, but it is not clear how it functions. Using live imaging and confocal microscopy we show that YB-1 functions only in the last step of division, specifically being required to initiate cytokinesis. ABSTRACTHigh levels of the cold shock protein Y-box-binding protein-1, YB-1, are tightly correlated with increased cell proliferation and cancer progression. However, the precise mechanism by which YB-1 regulates proliferation is unknown. Here, we found that YB-1 depletion in several cell lines resulted in cytokinesis failure, multinucleation and an increase in G1 transit time. Rescue experiments indicated that YB-1 was required for completion of cytokinesis. Using confocal imaging of cells undergoing cytokinesis both in vitro and in zebrafish embryos, we found that YB-1 was critical for microtubule organization during cytokinesis. Using mass spectrometry we identified multiple novel phosphorylation sites on YB-1. We show that phosphorylation of YB-1 at multiple serine residues was essential for its function during cytokinesis. Using atomistic modelling we show how multiple phosphorylations alter YB-1 conformation, allowing it to interact with protein partners. Our results establish phosphorylated YB-1 as a critical regulator of cytokinesis, defining for the first time precisely how YB-1 regulates cell division.Word Count 157 / 160 words

show abstract

“…Eomes and FoxH1 cooperate in controlling BMP/Nodal target genes together with Smads (Arnold et al, 2008;Bjornson et al, 2005;Miyazono et al, 2018;Slagle et al, 2011). Eomes, FoxH1, and Mixl1 have been implicated separately or in pairwise combinations in mesendoderm development Du et al, 2012;Henry and Melton, 1998;Kikuchi et al, 2000;Slagle et al, 2011;Xu et al, 2014). In particular, Eomes and MixL1 have been found to form a tripartite complex with Gata5 in early endoderm determination (Bjornson et al, 2005).…”

Section: A Transcription Factor Code For Mesendoderm-to-lpm Formationmentioning

confidence: 99%

A conserved regulatory program drives emergence of the lateral plate mesoderm

Hess

Nieuwenhuize

et al. 2018

Preprint

View full text Add to dashboard Cite

Abstract:Cardiovascular cell lineages emerge with kidney, smooth muscle, and limb skeleton progenitors from the lateral plate mesoderm (LPM). How the LPM emerges during development and how it has evolved to form key lineages of the vertebrate body plan remain unknown. Here, we captured LPM formation by transgenic in toto imaging and lineage tracing using the first pan-LPM enhancer element from the zebrafish gene draculin (drl). drl LPM enhancer-based reporters are specifically active in LPM-corresponding territories of several chordate species, uncovering a universal LPM-specific gene program. Distinct from other mesoderm, we identified EomesA, FoxH1, and MixL1 with BMP/Nodal-controlled Smad activity as minimally required factors to drive drl-marked LPM formation. Altogether, our work provides a developmental and mechanistic framework for LPM emergence and the in vitro differentiation of cardiovascular cell types. Our findings suggest that the LPM may represent an ancient cell fate domain that predates ancestral vertebrates.. CC-BY-NC-ND 4.0 International license peer-reviewed) is the author/funder. It is made available under aThe copyright holder for this preprint (which was not . http://dx.doi.org/10.1101/261115 doi: bioRxiv preprint first posted online Feb. 7, 2018; 2 Main Text:Following gastrulation, mesoderm in vertebrates is classically described to partition into axial, paraxial, and lateral domains (Gurdon, 1995). The ventrally forming lateral plate mesoderm (LPM) is composed of highly mobile cells and is mainly defined by its position adjacent to the somite-forming paraxial mesoderm (Hatada and Stern, 1994). Transplantation and lineage tracing experiments in several species have shown that the LPM contains progenitor cells of the circulatory system, smooth muscle lineages, the kidneys (often demarcated as dedicated intermediate mesoderm), and the limb anlagen (Chal and Pourquié, 2017; Gurdon, 1995; Takasato and Little, 2015). Several transcription factors including Hand1/2, Tbx5, Osr1, FoxF1, Prrx1, Mesp1, and Etv2 are expressed in LPM territories and play overlapping roles in cell fate determination (Chal and Pourquié, 2017;Davidson and Zon, 2004; Takasato and Little, 2015), albeit not always with conserved function (Yabe et al., 2016). Anterior-to-posterior expression domains of select transcription factors including Tbx1/10 and Hand are conserved in lampreys and even in the basal cephalochordate amphioxus that lacks major LPM-derived organ systems (Onimaru et al., 2011). These observations raise the possibility of the existence of an ancient upstream program that delineates the prospective LPM domain.In zebrafish, the LPM emerges from the ventral half of the embryo and becomes readily detectable as a patchwork of bilateral gene expression domains at the end of gastrulation (Davidson and Zon, 2004). hand2 expression marks in the anterior LPM (ALPM) the heart field and pectoral fin precursors, and a lateral-most domain within the posterior LPM (PLPM) (Perens et al., 2016; Yelon et al., 2000), wh...

show abstract

Maternal Eomesodermin regulates zygotic nodal gene expression for mesendoderm induction in zebrafish embryos

Cited by 30 publications

References 46 publications

A gene regulatory program controlling early Xenopus mesendoderm formation: Network conservation and motifs

A gene regulatory program controlling early Xenopus mesendoderm formation: Network conservation and motifs

Critical role for cold shock protein YB-1 in cytokinesis

A conserved regulatory program drives emergence of the lateral plate mesoderm

Contact Info

Product

Resources

About