Maternal dietary uridine supplementation reduces diarrhea incidence in piglets by regulating the intestinal mucosal barrier and cytokine profiles

Wu, Xin; Gao, Lumin; Liu, Yilin; Xie, Changqing; Cai, Lichuang; Xu, Kang; Zhou, Xin

doi:10.1002/jsfa.10410

Cited by 23 publications

(17 citation statements)

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“…As physiological factors, intestinal development, milk nutrients, stress factors, and feeding management could cause diarrhoea in piglets; thus, it is important to reduce diarrhoea in piglets by improving intestinal development. The results demonstrated dietary supplementation of uridine to sows could reduce the incidence of diarrhoea in suckling piglets ( Wu et al, 2020 ). A study on infants showed that nucleotide supplementation in infant formulas has anti-diarrhoeal effect and immune benefits ( Hess and Greenberg 2012 ).…”

Section: Discussionmentioning

confidence: 99%

“…Studies on sows have suggested that the growth performance of piglets was improved by maternal yeast culture supplementation during gestation and lactation ( Kim et al., 2008 ; Hung 2015 ). Furthermore, maternal dietary uridine supplementation reduces diarrhoea incidence in piglets by regulating the intestinal mucosal barrier and cytokine profiles ( Wu et al, 2020 ). In addition, studies on broilers and weaned piglets have suggested that exogenous nucleotides could improve growth performance, intestinal enzyme activity and villus development ( Superchi et al., 2012 ; Daneshmand et al., 2017 ).…”

Section: Introductionmentioning

confidence: 99%

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Yeast-based nucleotide supplementation in mother sows modifies the intestinal barrier function and immune response of neonatal pigs

Gao

Xie

Liang³

et al. 2021

Animal Nutrition

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In the present study, we aimed to evaluate the effects of maternal yeast-based nucleotide (YN) supplementation on the intestinal immune response and barrier function in neonatal pigs, as well as the diarrhoea rate and growth performance in suckling piglets. Sixty-four late-gestation sows were assigned to the following groups: the CON (fed a basal diet) and YN groups (fed a basal diet with 4 g YN/kg diet). The experiment started on d 85 of gestation and ended on d 20 of lactation. Diarrhoea rate and average daily gain of the piglets were recorded, and samples of blood and intestines from neonatal piglets were collected before they consumed colostrum during farrowing. Compared with the CON group, maternal YN supplementation increased the weaning weight of litter and decreased the diarrhoea rate ( P < 0.01). In addition, maternal YN supplementation promoted the ileal villus development in the neonates compared with that in the CON group ( P < 0.01). Maternal YN supplementation also increased the ileal secretory immunoglobulin A (sIgA) level compared with that in the CON group ( P < 0.05). The real-time PCR results showed that maternal dietary YN supplementation increased the jejunal and ileal expression of interleukin ( IL ) -17 , IL-8 , IL-1β , IL-10 and tumor necrosis factor ( TNF )- α in the neonates compared with that in the CON group ( P < 0.05). Overall, maternal nucleotide supplementation improved the villus development and innate immunity of neonatal piglets during late pregnancy. This may be associated with the decrease in diarrhoea and the increase in weaning weight of the litter of suckling piglets.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Yeast-based nucleotide supplementation in mother sows modifies the intestinal barrier function and immune response of neonatal pigs

Gao

Xie

Liang³

et al. 2021

Animal Nutrition

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“…Our previous studies showed that oral administration of uridine stimulated intestinal development, promoted nucleotide transport, improved growth performance, and regulated the fatty acid composition and lipid metabolism in weaned piglets [41][42][43][44]. We have demonstrated that maternal dietary supplement UR contributed to reducing the occurrence of diarrhea by regulating cytokine secretion and intestinal mucosal barrier function of suckling pigs [45]. A recent study demonstrated that uridine was able to inhibit stemness of intestinal stem cells in 3D intestinal organoids and mice [46].…”

Section: Relationships Among Uridine and Glucose Lipid And Amino Acmentioning

confidence: 99%

Uridine Metabolism and Its Role in Glucose, Lipid, and Amino Acid Homeostasis

Zhang

Guo

Xie

et al. 2020

BioMed Research International

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Pyrimidine nucleoside uridine plays a critical role in maintaining cellular function and energy metabolism. In addition to its role in nucleoside synthesis, uridine and its derivatives contribute to reduction of cytotoxicity and suppression of drug-induced hepatic steatosis. Uridine is mostly present in blood and cerebrospinal fluid, where it contributes to the maintenance of basic cellular functions affected by UPase enzyme activity, feeding habits, and ATP depletion. Uridine metabolism depends on three stages: de novo synthesis, salvage synthesis pathway and catabolism, and homeostasis, which is tightly relating to glucose homeostasis and lipid and amino acid metabolism. This review is devoted to uridine metabolism and its role in glucose, lipid, and amino acid homeostasis.

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“…During delivering, 5 ml blood was collected from eight sows per group from the jugular vein with a vacuum blood collection tube. In addition, on the day of birth, eight newborn male piglets (1•50 (SEM 0•204) kg) were randomly selected from each of the eight sows marked above as soon as they were born and they were kept apart from sows to avoid ingestion of colostrum (24) . And then, they were anaesthetised with an iv injection of sodium pentobarbital (50 mg/kg body weight) and bled by exsanguination (25) .…”

Section: Sample Collectionmentioning

confidence: 99%

“…Our previous research showed that UR had higher bioavailability than UMP in early weaned piglets (15) . Maternal dietary UR supplementation reduced diarrhoea incidence in piglets by regulating the intestinal mucosal barrier and cytokine profiles (24) , while this is the first research to study the effect of maternal dietary UR supplementation on reproductive performance in pigs. Previous studies reported that there is no effect on litter size and birth weight of piglets by supplementing yeast RNA with free nucleotides to sows 3 d before parturition (31) .…”

Section: Maternal Supplementation With Uridine Decreased Birth Mortalitymentioning

confidence: 99%

Maternal supplementation with uridine influences fatty acid and amino acid constituents of offspring in a sow–piglet model

et al. 2020

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Nucleotide plays an important role in the regulation of cellular energy and protein homeostasis. The objective of the current study was to investigate the cumulative effects of maternal supplementation with nucleotides in the form of uridine (UR) on fatty acids and amino acids constituents of neonatal piglets. A total of 52 pregnant sows with similar parity were assigned randomly and equally into the control (CON) group (fed a basal diet) or UR group (fed a basal diet with 150g/t UR). The experiment started on d 85 of gestation and ended on the day of delivery. The reproductive performance was recorded, and placenta, blood and liver samples of neonatal piglets were collected before consuming colostrum during farrowing. Results showed that supplementing with UR in sow’ diet significantly decreased the birth mortality of pigs (P = 0.05). In addition, maternal dietary UR supplementation increased serum total cholesterol (CHOL), high-density lipoprotein (HDL) and low-density lipoprotein (LDL) of neonatal piglets (P < 0.05). Moreover, the amino acid profile of serum and liver of neonatal piglets was affected by the addition of UR in sows’ diets (P<0.05). Furthermore, an up-regulation of mRNA expression of energy metabolism-related genes, including fatty acid elongase 5 (ELOVL5), fatty acid desaturase 1 (FADS1), hormone-sensitive lipase (HSL) and cholesterol- 7a-hydroxylase (CYP7a1), was observed in the liver of neonates from the UR group. Additionally, a decrease in placental gene expression of excitatory amino acid transporters 2 (EAAT2), excitatory amino acid transporters 3 (EAAT3), neutral AA transporter 1(LAT1) in UR group was concurrently observed (P<0.05), and higher protein expression of phosphorylated protein kinase B (P-AKT), raptor, peroxisome proliferator activated receptor α (PPARα) and PPARγ in placenta from UR group was also observed, (P<0.05). Together, these results showed that maternal UR supplementation could regulate the nutrients transport of placenta, largely in response to an alteration of mTORC1-PPARs signaling, thus regulating the nutrition metabolism of neonatal piglets, and improving reproductive performance.

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Maternal dietary uridine supplementation reduces diarrhea incidence in piglets by regulating the intestinal mucosal barrier and cytokine profiles

Cited by 23 publications

References 51 publications

Yeast-based nucleotide supplementation in mother sows modifies the intestinal barrier function and immune response of neonatal pigs

Yeast-based nucleotide supplementation in mother sows modifies the intestinal barrier function and immune response of neonatal pigs

Uridine Metabolism and Its Role in Glucose, Lipid, and Amino Acid Homeostasis

Maternal supplementation with uridine influences fatty acid and amino acid constituents of offspring in a sow–piglet model

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