Maternal dietary protein affects transcriptional regulation of myostatin gene distinctively at weaning and finishing stages in skeletal muscle of Meishan pigs

Sulforaphane causes a major epigenetic repression of myostatin in porcine satellite cells

“…Spiller et al 46 reported that one of bovine MSTN gene upstream regulatory elements appears to be critical for MSTN promoter activity and that MyoD interacts with this binding motif in vitro as well as in vivo to regulate MSTN gene expression. More recently, Liu et al 42 SFN and 5-aza-dC treatments clearly result in attenuated MSTN expression. In keeping with previous report that show that TSA increased MSTN mRNA expression up to 40-fold in C2C12 myoblast after treatment for 24 h, 40 a substantial increase in MSTN expression was observed in the TSA group.…”

“…42 Here, we experiments were repeated for three times with duplicates. Details of cell culture procedure are shown in Figure 1A.…”

DNA methylation dynamics during oocyte and embryo development and its associationwith environmental induced alterations

“…Several rodent models have been employed to investigate the epigenetic changes induced by a maternal HF diet. Results from a selection of models demonstrate that a HF diet during pregnancy can induce epigenetic modification, including changes in DNMT expression (Liu et al, 2011;Zhou and Pan, 2011), altered DNA methylation of genes involved in obesity, and energy homeostasis (Martínez et al, 2014) hypomethylation of the Mc4r gene, which plays a role in body weight regulation in mice (Gong et al, 2010); and altered transcription and methylation status of Igf-2 in fetal livers from dams fed HF diets (van Straten et al, 2010). Obviously studies on human tissues are more restrictive, however, recent studies employing genome-wide epigenetic analysis also revealed the potential for widespread DNA methylation variation in foetal tissues exposed to maternal gestational diabetes (Finer et al, 2015).…”

Maternal low-protein diet affects myostatin signaling and protein synthesis in skeletal muscle of offspring piglets at weaning stage

“…Muscle MSTN can be programmed by neonatal underfeeding [13] or can serve as a key mediator of inadequate prenatal nutrition [14] in rats. Feeding LP diet to sows throughout gestation and lactation produced offspring pigs with altered skeletal muscle MSTN expression distinctively at weaning and finishing stages [15]. However, it remains unclear whether alterations in MSTN expression may influence the MSTN signaling and thereby cause MSTN-regulated metabolic changes in skeletal muscle of offspring piglets.…”