Maternal creatine supplementation during pregnancy prevents acute and long-term deficits in skeletal muscle after birth asphyxia: a study of structure and function of hind limb muscle in the spiny mouse

LaRosa, Domenic A.; Ellery, Stacey J.; Snow, Rod J.; Walker, David W.; Dickinson, Hayley

doi:10.1038/pr.2016.153

Cited by 21 publications

(18 citation statements)

References 41 publications

(74 reference statements)

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“…Consequently, there is an increased demand for and utilization of creatine during pregnancy. Maternal creatine supplementation has been reported to improve neonatal survival and organ function following birth asphyxia in animals [228, 229, 231, 233–235, 237]. Human studies show changes in the maternal urine and plasma creatine levels across pregnancy and association to maternal diet [230, 232].…”

Section: Potential Medical Uses Of Creatinementioning

confidence: 99%

International Society of Sports Nutrition position stand: safety and efficacy of creatine supplementation in exercise, sport, and medicine

Kreider

Kalman²,

António

et al. 2017

Journal of the International Society of Sports Nutrition

443

602

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Creatine is one of the most popular nutritional ergogenic aids for athletes. Studies have consistently shown that creatine supplementation increases intramuscular creatine concentrations which may help explain the observed improvements in high intensity exercise performance leading to greater training adaptations. In addition to athletic and exercise improvement, research has shown that creatine supplementation may enhance post-exercise recovery, injury prevention, thermoregulation, rehabilitation, and concussion and/or spinal cord neuroprotection. Additionally, a number of clinical applications of creatine supplementation have been studied involving neurodegenerative diseases (e.g., muscular dystrophy, Parkinson’s, Huntington’s disease), diabetes, osteoarthritis, fibromyalgia, aging, brain and heart ischemia, adolescent depression, and pregnancy. These studies provide a large body of evidence that creatine can not only improve exercise performance, but can play a role in preventing and/or reducing the severity of injury, enhancing rehabilitation from injuries, and helping athletes tolerate heavy training loads. Additionally, researchers have identified a number of potentially beneficial clinical uses of creatine supplementation. These studies show that short and long-term supplementation (up to 30 g/day for 5 years) is safe and well-tolerated in healthy individuals and in a number of patient populations ranging from infants to the elderly. Moreover, significant health benefits may be provided by ensuring habitual low dietary creatine ingestion (e.g., 3 g/day) throughout the lifespan. The purpose of this review is to provide an update to the current literature regarding the role and safety of creatine supplementation in exercise, sport, and medicine and to update the position stand of International Society of Sports Nutrition (ISSN).

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Section: Potential Medical Uses Of Creatinementioning

confidence: 99%

International Society of Sports Nutrition position stand: safety and efficacy of creatine supplementation in exercise, sport, and medicine

Kreider

Kalman²,

António

et al. 2017

Journal of the International Society of Sports Nutrition

443

602

View full text Add to dashboard Cite

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“…Intrapartum asphyxia was induced at 38 days of gestation (term at 39 days) as previously described [17][18][19][20][21][22]. Briefly, pregnant dams were killed via cervical dislocation.…”

Section: Housing Facility and Animal Carementioning

confidence: 99%

“…This study used the precocial spiny mouse to study the effects of intrapartum asphyxia on the brain [17][18][19][20][21][22]. The spiny mouse differs from conventional laboratory rodents (mice, rats) in that considerable brain maturation (including the beginnings of myelination) occurs in utero before birth [47]; hence, acute hypoxia delivered during the intrapartum period can be used as a model of oxygen starvation that occurs at birth in some human pregnancies.…”

Section: Strengths and Limitationsmentioning

confidence: 99%

“…Using a well-validated model of intrapartum asphyxia in the spiny mouse [17][18][19][20][21][22], we investigated the effects of 3K3A-APC treatment on the neuropathology that follows brief asphyxia, in comparison with vehicle (saline) or wtAPC treatments. Specifically, our aim was to assess the cerebral effects of 3K3A-APC in both the gray and white matter by examining markers of neuro-inflammation and cell death.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of 3K3A-Activated Protein C to Treat Neonatal Hypoxic Ischemic Brain Injury in the Spiny Mouse

et al. 2019

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Neonatal hypoxic ischemic encephalopathy (HIE) resulting from intrapartum asphyxia is a global problem that causes severe disabilities and up to 1 million deaths annually. A variant form of activated protein C, 3K3A-APC, has cytoprotective properties that attenuate brain injury in models of adult stroke. In this study, we compared the ability of 3K3A-APC and APC (wild-type (wt)) to attenuate neonatal brain injury, using the spiny mouse (Acomys cahirinus) model of intrapartum asphyxia. Pups were delivered at 38 days of gestation (term = 39 days), with an intrapartum hypoxic insult of 7.5 min (intrapartum asphyxia cohort), or immediate removal from the uterus (control cohort). After 1 h, pups received a subcutaneous injection of 3K3A-APC or wild-type APC (wtAPC) at 7 mg/kg, or vehicle (saline). At 24 h of age, pups were killed and brain tissue was collected for measurement of inflammation and cell death using RT-qPCR and histopathology. Intrapartum asphyxia increased weight loss, inflammation, and apoptosis/necrosis in the newborn brain. 3K3A-APC administration maintained body weight and ameliorated an asphyxia-induced increase of TGFβ1 messenger RNA expression in the cerebral cortex, immune cell aggregation in the corpus callosum, and cell death in the deep gray matter and hippocampus. In the cortex, 3K3A-APC appeared to exacerbate the immune response to the hypoxic ischemic insult. While wtAPC reduced cell death in the corpus callosum and hippocampus following intrapartum asphyxia, it increased markers of neuro-inflammation and cell death in control pups. These findings suggest 3K3A-APC administration may be a useful therapy to reduce cell death and neonatal brain injury associated with HIE.

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“…, ; LaRosa et al . ). A particular benefit in relation to CP is not only the decrease in severity of brain damage, but also the protection of skeletal muscle fibres and improved resistance to fatigue in the diaphragm (LaRosa et al .…”

Section: Introductionmentioning

confidence: 97%

Antenatal prevention of cerebral palsy and childhood disability: is the impossible possible?

Ellery

Kelleher

Grigsby

et al. 2018

The Journal of Physiology

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This review covers our current knowledge of the causes of perinatal brain injury leading to cerebral palsy-like outcomes, and argues that much of this brain damage is preventable. We review the experimental evidence that there are treatments that can be safely administered to women in late pregnancy that decrease the likelihood and extent of perinatal brain damage that occurs because of acute and severe hypoxia that arises during some births, and the additional impact of chronic fetal hypoxia, infection, inflammation, growth restriction and preterm birth. We discuss the types of interventions required to ameliorate or even prevent apoptotic and necrotic cell death, and the vulnerability of all the major cell types in the brain (neurons, astrocytes, oligodendrocytes, microglia, cerebral vasculature) to hypoxia/ischaemia, and whether a pan-protective treatment given to the mother before birth is a realistic prospect.

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Maternal creatine supplementation during pregnancy prevents acute and long-term deficits in skeletal muscle after birth asphyxia: a study of structure and function of hind limb muscle in the spiny mouse

Abstract: This study demonstrates that creatine loading before birth protects the muscle from asphyxia-induced damage at birth.

Cited by 21 publications

References 41 publications

International Society of Sports Nutrition position stand: safety and efficacy of creatine supplementation in exercise, sport, and medicine

International Society of Sports Nutrition position stand: safety and efficacy of creatine supplementation in exercise, sport, and medicine

Evaluation of 3K3A-Activated Protein C to Treat Neonatal Hypoxic Ischemic Brain Injury in the Spiny Mouse

Antenatal prevention of cerebral palsy and childhood disability: is the impossible possible?

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