Maternal body mass index affects embryo morphokinetics: a time-lapse study

Bartolacci, Alessandro; Buratini, J.; Moutier, Clarissa; Guglielmo, Maria Cristina; Novara, Paola; Brambillasca, Fausta; Renzini, Mario Mignini; Canto, Mariabeatrice Dal

doi:10.1007/s10815-019-01456-3

Cited by 43 publications

(27 citation statements)

References 59 publications

(80 reference statements)

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“…In contrast, a recent study including 1528 patients reported that t5 was longer in overweight women and t5 with t8 was longer in obese compared to non-obese patients. 59 Conversely, BMI was not noted to affect embryo development timing points in an analysis performed retrospectively on 89 patients. 60 …”

Section: Confounding Factorsmentioning

confidence: 89%

The clinical use of time-lapse in human-assisted reproduction

Minasi

Greco

Varricchio

et al. 2020

Clin Med�Insights�Reprod�Health

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A major challenge in the assisted reproduction laboratory is to set up reproducible and efficient criteria to identify the embryo with the highest developmental potential. Over the years, several methods have been used worldwide with this purpose. Initially, standard morphology assessment was the only available strategy. It is now universally recognized that besides being a very subjective embryo selection strategy, morphology evaluation alone has a very poor prognostic value. More recently, the availability of time-lapse incubators allowed a continuous monitoring of human embryo development. This technology has spread quickly and many fertility clinics over the world produced a remarkable amount of data. To date, however, a general consensus on which variables, or combination of variables, should play a central role in embryo selection is still lacking. Many confounding factors, concerning both patient features and clinical and biological procedures, have been observed to influence embryo development. In addition, several studies have reported unexpected positive outcomes, even in the presence of abnormal developmental criteria. While it does not seem that time-lapse technology is ready to entirely replace the more invasive preimplantation genetic testing in identifying the embryo with the highest implantation potential, it is certainly true that its application is rapidly growing, becoming progressively more accurate. Studies involving artificial intelligence and deep-learning models as well as combining morphokinetic with other non-invasive markers of embryo development, are currently ongoing, raising hopes for its successful applicability for clinical purpose in the near future. The present review mainly focuses on data published starting from the first decade of 2000, when time-lapse technology was introduced as a routine clinical practice in the infertility centers.

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Section: Confounding Factorsmentioning

confidence: 89%

The clinical use of time-lapse in human-assisted reproduction

Minasi

Greco

Varricchio

et al. 2020

Clin Med�Insights�Reprod�Health

View full text Add to dashboard Cite

show abstract

“…However, some scholars suggested that the BMI had no effect on the embryo morphokinetic parameters and embryonic development [21] . Bartolacci et al elucidated the correlation between the BMI and the embryo morphokinetic parameters using the time-lapse monitoring system and found that the embryo development in overweight or obese females was slower (t5 and t8 were prolonged), and these morphokinetic parameters may be used to improve the selection of the high-quality embryos [22] . Therefore, the present study further analyzed the participants with different BMI and found that the Day 3 high-quality embryo ratio and the blastocyst formation rate were not statistically different between participants with different BMI (P > 0.05).…”

Section: Discussionmentioning

confidence: 99%

Embryo Morphokinetic Parameters Evaluated Using a Time-Lapse Embryo Monitoring System Can Predict the Embryo Developmental Potential in ICSI Cycles

Yao

et al. 2021

Preprint

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At present, embryo morphology assessment based on the observation of the embryonic morphological characteristics at several specific time points has been mainly used for selecting the high-quality embryo. However, we all know that embryo development is a dynamic process. Many research results on the correlation between the embryo morphokinetic parameters and embryo quality and development potential were inconsistent. With the help of time-lapse imaging, the development processes and outcomes of a total of 365 embryos were cultured and analyzed in this study. The results showed that the mean tPNf and t2 of the high-quality embryo were significantly shorter than the low-quality embryo; the mean t2PBe and tPNa of the high-quality embryo from the implantation group were significantly shorter than those from the non-implantation group. In addition, based on the quartile grouping of each morphokinetic parameter, the embryos that had 21.15≤tPNf≤25.30 value were significantly higher in embryo quality when compared with the embryos that had the tPNf values outside the range on Days 3. Similarly, the embryos that had values of t2≤25.60 were significantly higher in embryo quality than those with outside the range values on Days 3. Thus, we demonstrated that the morphokinetic parameter evaluated using a time-lapse embryo monitoring system can predict the embryo quality, and be benefit for the selection of the high-quality embryos and improvement for the implantation success of the patient in assisted reproductive technologies.

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“…Overall, we observed similar embryo morphokinetic parameters between the two study groups, but a significantly higher proportion of embryos displaying an optimal cleavage range in the very early stages of embryo development (t2 and t3) was observed in the MI + ALA + FA group compared to the FA-only group, revealing a potential, although limited, positive impact on embryo kinetics. Interestingly enough, it was previously observed that embryo morphokinetics at the cleavage stage are significantly slower in obese compared with normal-weight women [ 61 ], and that embryo development from hyperandrogenic PCOS women is significantly delayed at early stages compared with embryos from non-PCOS, regularly cycling women [ 62 ]. Taken together, these data reveal that causative factor for subfertility in overweight/obese or PCOS women may be related, at least in part, to the developmental timing of pre-implantation embryos.…”

Section: Discussionmentioning

confidence: 99%

Effect of a Combination of Myo-Inositol, Alpha-Lipoic Acid, and Folic Acid on Oocyte Morphology and Embryo Morphokinetics in non-PCOS Overweight/Obese Patients Undergoing IVF: A Pilot, Prospective, Randomized Study

Canosa

Paschero

Carosso

et al. 2020

JCM

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Herein we aimed at assessing whether Myo-Inositol (MI), Alpha–Lipoic acid (ALA), and Folic acid (FA) could improve oocyte quality and embryo development in non-PCOS overweight/obese women undergoing IVF. Three hundred and twenty-four mature oocytes were obtained from non-PCOS overweight/obese patients, randomized to receive either MI, ALA, and FA (MI + ALA + FA group, n = 155 oocytes) or FA alone (FA-only group, n = 169 oocytes). Oocytes were examined using Polarized Light Microscopy to assess morphological features of zona pellucida (ZP) and meiotic spindle (MS). One hundred and seventy-six embryos (n = 84 in the MI + ALA + FA group, n = 92 in the FA-only group) were assessed by conventional morphology on days 2 and 5, as well as using the Time-Lapse System morphokinetic analysis. A significantly higher ZP retardance, area, and thickness (p < 0.05), and a shorter MS axis (p < 0.05) were observed in the MI + ALA + FA group, suggesting a positive effect on oocyte quality. Conventional morphology evaluation on day 2 showed a higher mean embryo score in the MI + ALA + FA group, whereas embryo morphokinetic was comparable in the two groups. Overall, our data show a possible beneficial effect of the combination of MI, ALA, and FA on oocyte and embryo morphology, encouraging testing of this combination in adequately powered randomized trials to assess their impact of clinical IVF results.

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Maternal body mass index affects embryo morphokinetics: a time-lapse study

Cited by 43 publications

References 59 publications

The clinical use of time-lapse in human-assisted reproduction

The clinical use of time-lapse in human-assisted reproduction

Embryo Morphokinetic Parameters Evaluated Using a Time-Lapse Embryo Monitoring System Can Predict the Embryo Developmental Potential in ICSI Cycles

Effect of a Combination of Myo-Inositol, Alpha-Lipoic Acid, and Folic Acid on Oocyte Morphology and Embryo Morphokinetics in non-PCOS Overweight/Obese Patients Undergoing IVF: A Pilot, Prospective, Randomized Study

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