Maternal Betaine Supplementation Mitigates Maternal High Fat Diet-Induced NAFLD in Offspring Mice through Gut Microbiota

Sun, Liuqiao; Tan, Xuying; Li, Xiaoping; Chen, Hangjun; Ou, Qian; Wu, Qiongmei; Yu, Xinxue; Zhao, Haitao; Huang, Qiaoli; Yi, Zehua; Wei, Jintao; Wu, Feng; Zhu, Huilian; Wang, Lijun

doi:10.3390/nu15020284

Cited by 12 publications

(9 citation statements)

References 49 publications

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“…The putative mechanisms underlying the effects of betaine on lipid metabolism included changes in gut microbiota (i.e., reductions in Intestinimonas, Acetatifactor, and Desulfovibrio, and increases in Lachnoclostridium and Romboutsia), with intergenerational metabolic impacts [49]. In addition, maternal betaine supplementation has been shown to reduce hepatic triglyceride content in offspring mice by decreasing the relative abundances of Proteobateria, Desulfovibrio, and Ruminococcus, but increasing Bacteroides and Parabacteroides [50]. In addition, betaine alleviated diet-induced disruption of hepatic lipid metabolism in mice, which was related to the modification of methylation on lipogenic genes [51].…”

Section: Discussionmentioning

confidence: 99%

Low Dietary Betaine Intake Is Associated with Increased Blood Cholesterol in Mexican Subjects

Ramos-Lopez,

Santuario-Loera

2024

Healthcare

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Background: Betaine, an osmolyte derivative of the metabolite choline and the amino acid glycine, acts as a methyl donor in the conversion of homocysteine to methionine and is involved in the maintenance of adequate lipid metabolism. There is growing evidence for the role of betaine in the development of various lipid-related diseases, including dyslipidemia and cardiovascular risk. This study aimed to analyze associations between betaine intake and blood lipid profiles in Mexican subjects. Methods: A total of 212 adults were randomly recruited in the city of Tijuana, Baja California, Mexico. Betaine intake was estimated using Nutritionist Pro software. Body composition and metabolic measurements were obtained by conventional methods. In the total sample, the average intake of betaine was 14.32 mg/d. Individuals were categorized into three groups according to tertiles of betaine consumption: tertile/group 1 (<4.16 mg/d), tertile/group 2 (4.16–12.02 mg/d), and tertile/group 3 (>12.02 mg/d). Results: Compared to group 3, subjects within group 1 had higher serum levels of total cholesterol (p = 0.001), LDL-c (p = 0.026), and non-HDL-c (p = 0.021). In addition, significant negative Pearson correlations were found between betaine intake and the serum levels of total cholesterol (r = −0.432, 95% CI, −0.684, −0.185, p = 0.001), LDL-c (r = −0.370, 95% CI, −0.606, −0.134, p = 0.002), and non-HDL-c (r = −0.351, 95%CI, −0.604, −0.098, p = 0.007). Conclusions: Our results show that a low intake of betaine is associated with elevated blood cholesterol levels in Mexican subjects. On this basis, betaine consumption could be used as an additional dietary measure for cardiovascular care. However, additional studies are required to confirm our results in other Mexican regions as well as in other populations worldwide.

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Section: Discussionmentioning

confidence: 99%

Low Dietary Betaine Intake Is Associated with Increased Blood Cholesterol in Mexican Subjects

Ramos-Lopez,

Santuario-Loera

2024

Healthcare

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“…Betaine is a growing biological methyl donor ( Bekdash, 2023 ). Betaine increases energy metabolism enzyme activity and reduces liver fat in hens ( Sun L et al, 2023 ). Betaine-supplemented hens have improved growth rate, feed efficiency, and carcass quality, especially during heat stress or disease outbreaks ( Suliman et al, 2023 ; Sun S et al, 2023 ).…”

Section: Discussionmentioning

confidence: 99%

The effects of essential oil from Lippia origanoides and herbal betaine on performance, intestinal integrity, bone mineralization and meat quality in broiler chickens subjected to cyclic heat stress

Señas-Cuesta,

Stein,

Latorre

et al. 2023

Front. Physiol.

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Essential oils (EO) affect performance, intestinal integrity, bone mineralization, and meat quality in broiler chickens subjected to cyclic heat stress (HS). Day-of-hatch Cobb 500 male broiler chicks (n = 475) were randomly divided into four groups. Group 1: No heat stress (Thermoneutral) + control diets with no antibiotics; Group 2: heat stress control + control diets; Group 3: heat stress + control diets supplemented with thymol chemotype (45 ppm) and herbal betaine (150 ppm) formulation EO1; Group 4: heat stress + control diets supplemented with phellandrene (45 ppm) and herbal betaine (150 ppm) formulation EO2. From day 10–42, the heat stress groups were exposed to cyclic HS at 35°C for 12 h (8:00–20:00). BW, BWG, FI, and FCRc were measured at d 0, 10, 28, and 42. Chickens were orally gavaged with FITC-d on days 10 (before heat stress) and 42. Morphometric analysis of duodenum and ileum samples and bone mineralization of tibias were done. Meat quality was assessed on day 43 with ten chickens per pen per treatment. Heat stress reduced BW by day 28 (p < 0.05) compared to thermoneutral chickens. At the end of the trial, chickens that received both formulations of EO1 and EO2 had significantly higher BW than HS control chickens. A similar trend was observed for BWG. FCRc was impaired by EO2 supplementation. There was a significant increase in total mortality in EO2 compared with EO1 EO1 chickens had lower FITC-d concentrations at day 42 than the HS control. In addition, EO1 treatment is not statistically different if compared to EO2 and thermoneutral. Control HS broilers had significantly lower tibia breaking strength and total ash at day 42 than heat-stressed chickens supplemented with EO1 and EO2. Heat stress affected intestinal morphology more than thermoneutral chickens. EO1 and EO2 improved intestinal morphology in heat-stressed chickens. Woody breast and white striping were more common in thermoneutral chickens than heat stress chickens. In conclusion, the EO-containing diet could improve broiler chicken growth during cyclic heat stress, becoming increasingly relevant in antibiotic-free production in harsh climates.

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“…Gut microbiota of infants born from obese mothers increased the susceptibility to NAFLD in the mice through fecal microbiota transplantation experiments (Soderborg et al, 2018). Our previous research also found that maternal HFD changed the distribution of gut microbiota and induced NAFLD in offspring mice (Sun et al, 2023). Bifidobacteria and Lactobacillus are recognized as beneficial microbiota in the gut.…”

Section: Introductionmentioning

confidence: 90%

Gestational supplementation of Bifidobacterium, Lactobacillus, and Streptococcus thermophilus attenuates hepatic steatosis in offspring mice through promoting fatty acid β‐oxidation

Chen,

Wu,

Chen

et al. 2024

Journal of Food Science

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Currently, Bifidobacterium, Lactobacillus, and Streptococcus thermophilus (BLS) are widely recognized as the crucially beneficial bacteria in the gut. Many preclinical and clinical studies have shown their protective effects against non‐alcoholic fatty liver disease (NAFLD). However, whether gestational BLS supplementation could alleviate NAFLD in the offspring is still unknown. Kunming mice were given a high‐fat diet (HFD) for 4 weeks before mating. They received BLS supplementation by gavage during pregnancy. After weaning, offspring mice were fed with a regular diet up to 5 weeks old. Gestational BLS supplementation significantly increased the abundance of Actinobacteriota, Bifidobacterium, and Faecalibaculum in the gut of dams exposed to HFD. In offspring mice exposed to maternal HFD, maternal BLS intake significantly decreased the ratio of Firmicutes to Bacteroidetes as well as the relative abundance of Prevotella and Streptococcus, but increased the relative abundance of Parabacteroides. In offspring mice, maternal BLS supplementation significantly decreased the hepatic triglyceride content and mitigated hepatic steatosis. Furthermore, maternal BLS supplementation increased the glutathione content and reduced malondialdehyde content in the liver. In addition, mRNA and protein expression levels of key rate‐limiting enzymes in mitochondrial β‐oxidation (CPT1α, PPARα, and PGC1α) in the livers of offspring mice were significantly increased after gestational BLS supplementation. Thus, gestational BLS supplementation may ameliorate maternal HFD‐induced steatosis and oxidative stress in the livers of offspring mice by modulating fatty acid β‐oxidation.

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Maternal Betaine Supplementation Mitigates Maternal High Fat Diet-Induced NAFLD in Offspring Mice through Gut Microbiota

Cited by 12 publications

References 49 publications

Low Dietary Betaine Intake Is Associated with Increased Blood Cholesterol in Mexican Subjects

Low Dietary Betaine Intake Is Associated with Increased Blood Cholesterol in Mexican Subjects

The effects of essential oil from Lippia origanoides and herbal betaine on performance, intestinal integrity, bone mineralization and meat quality in broiler chickens subjected to cyclic heat stress

Gestational supplementation of Bifidobacterium, Lactobacillus, and Streptococcus thermophilus attenuates hepatic steatosis in offspring mice through promoting fatty acid β‐oxidation

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