Maternal and foetal cytokine production in dams naturally and experimentally infected with Neospora caninum on gestation day 110

Darwich, Laila; Li, Yanli; Serrano-Pérez, B.; Mur-Novales, R.; García-Ispierto, Irina; Cabezón, Óscar; López‐Gatius, F.; Almerı́a, S.

doi:10.1016/j.rvsc.2016.05.003

Cited by 9 publications

(11 citation statements)

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“…The aim of this study was to reproduce N. caninum infections in goats under analogous experimental conditions used in sheep for proper comparison with experimental neosporosis in sheep [ 15 ] and cattle [ 19 , 21 ]. The crucial role played by time of pregnancy for infection in the outcome of neosporosis was also demonstrated in goats.…”

Section: Discussionmentioning

confidence: 99%

Experimental caprine neosporosis: the influence of gestational stage on the outcome of infection

Porto

Regidor‐Cerrillo

Kim

et al. 2016

Vet Res

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Here, we assessed outcome of experimental infection by Neospora caninum in goats intravenously inoculated with 106 tachyzoites of the Nc-Spain7 isolate at 40 (G1), 90 (G2) and 120 (G3) days of gestation. Infected goats had fever between 5 and 9 days post inoculation (dpi); all were seropositive at the time of abortion/birth. Foetal death occurred in G1 from 10 to 21 dpi (n = 7) and in G2 from 27 to 35 dpi (n = 4). Goats in G2 also had seropositive stillbirth (n = 1) and healthy kids (n = 2). G3 goats (n = 7) had 3 seropositive and 3 seronegative weak kids, and 2 seronegative healthy kids. Parasite DNA detection in placentomes was 100% in G2, 85.7% in G3 and in G1 was detected only in placentomes from the goats with foetal losses from 17 dpi (100%). Parasites were detected in foetal/kid brain (>85.7%) and liver (≥50%) of G2 and G3, and in G1 after 17 dpi (100%). The highest parasite loads were detected in the placentomes of G1 from 17 dpi and G2, and in foetal tissues of G1 from 17 dpi and G3. Multifocal necrotic lesions were observed in the placentas of the three groups, but they were larger and more frequent in G1 and G2. Similar lesions were observed in foetal tissues, but they were more frequent in G3. These findings suggest that, as observed in cattle and sheep, the clinical consequences of N. caninum in pregnant goats are dependent in part on the time of gestation when animals were infected.Electronic supplementary materialThe online version of this article (doi:10.1186/s13567-016-0312-6) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Experimental caprine neosporosis: the influence of gestational stage on the outcome of infection

Porto

Regidor‐Cerrillo

Kim

et al. 2016

Vet Res

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“…Com relação à resposta imunológica contra Nc durante a prenhez, estudos demonstram que, além das citocinas Th1 (principalmente IFN-γ), citocinas do padrão Th2 e Treg também são reguladas positivamente durante o curso de infecção experimental (ALMERÍA et al, 2014). Sabe-se que a imunorregulação que ocorre na interface materno-fetal, com a expressão aumentada de IL-10, TGF-β, IL-4 e IL-5, pondera os efeitos das citocinas pró-inflamatórias, conferindo proteção ao feto (INNES, 2007;ALMERIA et al, 2016;DARWICH et al, 2016). Em contrapartida, na infecção por N. caninum, essa modulação da resposta Th1 contra o parasita favorece a proliferação do parasita e a transmissão vertical, podendo afetar a saúde do feto MILLER;ELLIS, 2004;INNES, 2007).…”

Section: A Resposta Imune Contra a Infecção Por N Caninumunclassified

“…Foi observado que células esplênicas de camundongos resistentes à infecção mostraram uma alta razão IFN-γ/IL-4 na produção de citocinas, enquanto uma baixa razão IFN-γ/IL-4 foi associada à suscetibilidade à infecção (LONG; BASZLER; MATHISON, 1998). Em contrapartida, em modelos de prenhez bovina com infecção por Nc, a razão IFN-γ/IL-4 no sangue periférico foi associada ao sucesso na gestação (DARWICH et al, 2016). Foi observada também resposta imune mediada por células, caracterizada pela produção de IL-4 e decréscimo da produção de IFN-γ, IL-12 e TNFα (QUINN; MILLER; ELLIS, 2004).…”

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Função da galectina-1 na resistência do hospedeiro contra a infecção experimental por Neospora caninum

Ferreira¹

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“…It is well established that the IFNγ is an important proinflammatory cytokine for the parasite replication control through the induction of effector molecules such as nitric oxide (NO) and reactive oxygen species (ROS) and acts as an Th-1-subtype lymphocytes inducer (BAZLER et al, 1999). The IFNγ production is also critical for infection control in cattle (INNES et al, 1995;DARWICH et al, 2016). However, although IFNγ is crucial for the host, during gestation the response generated by this cytokine can cause fetal rejection or abortion due to the induced inflammatory response (KHAN et al, 1997;BASZLER et al, 1999;QUINN et al, 2002;ROSBOTTOM et al, 2008;ALMERIA et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Estimulação de vias inatas em células uterinas e ensaios de imunoprofilaxia em modelo murino visando o controle da neosporose bovina

Ramos¹

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Neospora caninum has caused great interest in the research due to its association with repeated abortion in the cattle herd, entailing billions of dollars of loss in livestock annually. Classified as a mandatory intracellular protozoan, vertical transmission is common in bovine hosts and the consequences generated will depend on the host immune response profile. In addition, N. caninum presents several secretory molecules important in the invasion process. Among them, NcROP4 is a rhoptry protein that has immunogenic character important for the process of invasion and evasion in the host cell. Here we aimed at two manuscripts to understand the innate mechanisms involved in the response against N. caninum in bovine uterine cells and to evaluate the immunomodulatory capacity of recombinant NcROP4 (rNcROP4) in the development of vaccine protocols and from there to test the immunotherapeutic protocols of an acute inflammatory pathology. The

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Maternal and foetal cytokine production in dams naturally and experimentally infected with Neospora caninum on gestation day 110

Cited by 9 publications

References 50 publications

Experimental caprine neosporosis: the influence of gestational stage on the outcome of infection

Experimental caprine neosporosis: the influence of gestational stage on the outcome of infection

Função da galectina-1 na resistência do hospedeiro contra a infecção experimental por Neospora caninum

Estimulação de vias inatas em células uterinas e ensaios de imunoprofilaxia em modelo murino visando o controle da neosporose bovina

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