Materials characterization and mechanobiology of the eye

Hugar, Daniel L.; Ivanisevic, Albena

doi:10.1016/j.msec.2013.02.009

Cited by 11 publications

(12 citation statements)

References 158 publications

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“…We hypothesized that the rigidity of the 1% (w/v) agarose gel (measured at 15.5 ± 1.2 kPa) impeded retinal tissue growth and, by extension, cell movement over time. Given the lower Young's modulus (≈1.1 kPa) of the 0.75% (w/v) IEDDA HA–HA (24/240) hydrogels, which is similar to that of retinal tissue,68 coupled with a usable gelation time and absence of bubbles, we tested it as a replacement for agarose for retinal explant embedding. The hydrogel was applied as a viscous solution (5 to 6 min after mixing) over flattened explants on a 100 µm cell strainer membrane, and then gelled at room temperature, thereby obviating the need for heating and cooling that is required with agarose.…”

Section: Resultsmentioning

confidence: 99%

“…[38] However, long-term (>12 h) imaging and cell tracking of fluorescent reporter signals using this technique are challenged by retinal explant viability, photodamage, and loss of fluorescence signal. Although it is necessary to immobilize retinal explants for time-series image acquisition, embedding in standard agarose thermogels is made more difficult by the growth of the retinal explant and the physical constraints of agarose, thereby limiting the effective imaging window to ≈20 h. [38] We hypothesized that the rigidity of the 1% (w/v) agarose gel (measured at 15.5 ± 1. the 0.75% (w/v) IEDDA HA-HA (24/240) hydrogels, which is similar to that of retinal tissue, [68] coupled with a usable gelation time and absence of bubbles, we tested it as a replacement for agarose for retinal explant embedding. The hydrogel was applied as a viscous solution (5 to 6 min after mixing) over flattened explants on a 100 µm cell strainer membrane, and then gelled at room temperature, thereby obviating the need for heating and cooling that is required with agarose.…”

Section: Iedda Ha-ha Hydrogels Improve Retinal Explant Multiphoton Immentioning

confidence: 99%

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Nonswelling, Ultralow Content Inverse Electron‐Demand Diels–Alder Hyaluronan Hydrogels with Tunable Gelation Time: Synthesis and In Vitro Evaluation

Delplace

Nickerson

Ortín-Martínez

et al. 2020

Adv Funct Materials

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Hyaluronan (HA) is a major component of the extracellular matrix and is particularly attractive for cell‐based assays; yet, common crosslinking strategies of HA hydrogels are not fully tunable and bioorthogonal, and result in gels subject to swelling, which affects their physicochemical properties. To overcome these limitations, HA hydrogels based on the inverse electron‐demand Diels–Alder (IEDDA) “click” reaction are designed. By crosslinking two modified HA components together, as opposed to using telechelic components, tunable gelation times as fast as 4.4 ± 0.4 min and as slow as 46.2 ± 1.8 min are achieved for facile use. By optimizing HA molar mass, ultralow polymer content hydrogels of 0.5% (w/v), resulting in minimal (<3–5% mass variation) to nonswelling (<1%), transparent and biodegradable hydrogels are synthesized. To demonstrate their versatility, the newly designed hydrogels are tested as matrices for 3D cell culture and retinal explant imaging where transparency is important. IEDDA hydrogels are cytocompatible with primary photoreceptors and enable multiphoton imaging of embedded retinal explants for double the time (>38 h) than agarose thermogels (<20 h). IEDDA HA hydrogels constitute a new hydrogel platform. They have low polymer content, tunable gelation time, and are stable, thereby making them suitable for a diversity of applications.

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Section: Resultsmentioning

confidence: 99%

Section: Iedda Ha-ha Hydrogels Improve Retinal Explant Multiphoton Immentioning

confidence: 99%

Nonswelling, Ultralow Content Inverse Electron‐Demand Diels–Alder Hyaluronan Hydrogels with Tunable Gelation Time: Synthesis and In Vitro Evaluation

Delplace

Nickerson

Ortín-Martínez

et al. 2020

Adv Funct Materials

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show abstract

“…The properties of the ECM can vary considerably between species 47,49,50 , and each layer in the rabbit eye is consistently softer than the corresponding structure in the human eye 47 . For further information, we direct the reader to these reviews on the nuances of stiffness measurements in ocular tissues 42,44 and comprehensive summaries of biomechanical measurements of ocular tissue 51–55 .…”

Section: Methods For Characterizing the Biophysical Properties Of mentioning

confidence: 99%

Biomechanical relationships between the corneal endothelium and Descemet's membrane

Ali

Raghunathan

et al. 2016

Experimental Eye Research

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The posterior face of the cornea consists of the corneal endothelium, a monolayer of cuboidal cells that secrete and attach to Descemet’s membrane, an exaggerated basement membrane. Dysfunction of the endothelium compromises the barrier and pump functions of this layer that maintain corneal deturgesence. A large number of corneal endothelial dystrophies feature irregularities in Descemet’s membrane, suggesting that cells create and respond to the biophysical signals offered by their underlying matrix. This review provides an overview of the bidirectional relationship between Descemet’s membrane and the corneal endothelium. Several experimental methods have characterized a richly topographic and compliant biophysical microenvironment presented by the posterior surface of Descemet’s membrane, as well as the ultrastructure and composition of the membrane as it builds during a lifetime. We highlight the signaling pathways involved in the mechanotransduction of biophysical cues that influence cell behavior. We present the specific example of Fuchs’ Corneal Endothelial Dystrophy as a condition in which a dysregulated Descemet’s membrane may influence the progression of disease. Finally, we discuss some disease models and regenerative strategies that may facilitate improved treatments for corneal dystrophies.

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“…Some authors suggested the use of ex vivo (animal) eyes for a preliminary evaluation of the mechanical compatibility between experimental keratoprosthetic skirt and corneal tissue . From a general viewpoint, it cannot be ignored that BGs and BGCs can have a generally good mechanical compatibility with the orbital bone but are remarkably stiffer, even if produced in a porous form, than the ocular and intraorbital tissues (Table ) , . Therefore, future research directions could be addressed to the development of more compliant ocular biomaterials, such as BG/polymer constructs that have already been successfully experimented in ophthalmic surgery …”

Section: Criteria and Guidelines For Selecting And Testing Suitable Bmentioning

confidence: 99%

“…111 From a general viewpoint, it cannot be ignored that BGs and BGCs can have a generally good mechanical compatibility with the orbital bone but are remarkably stiffer, even if produced in a porous form, than the ocular and intraorbital tissues (Table III). 75,[112][113][114][115] Therefore, future research directions could be addressed to the development of more compliant ocular biomaterials, such as BG/polymer constructs that have already been successfully experimented in ophthalmic surgery. 14,19,30,51 According to the majority of scientists, after extensive in vitro tests with cells there is no recognized alternative to preclinical animal trials in order to progress the development of biomedical implants toward the final stage of clinical trials (human patients).…”

Section: Testing and Validationmentioning

confidence: 99%

How can bioactive glasses be useful in ocular surgery?

Baino

2014

J Biomedical Materials Res

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In the last few decades, the introduction of bioactive glasses (BGs), a special class of bioceramics that are able to bond to living tissues stimulating new tissue growth, has improved both treatment procedures via reconstructive surgery and the quality of life of rehabilitated patients in orthopedics and dentistry. While BGs have been extensively investigated for applications in these two surgical fields, there has been relatively little research on their use in other medical areas. Glass has been used for centuries to produce external refractive lenses and the intraocular implantation of small glass disks to correct visual deficiencies has been documented since the mid 1700s. Moreover, some evidences reported in the recent literature seem to demonstrate that the success of three specific types of ophthalmic devices, that is, synthetic grafts for eye orbit bone repair, orbital implants replacing the whole ocular globe and keratoprostheses (artificial cornea), could significantly benefit by the use of BG. A prospective view as well as a state-of-the-art review on this topic are currently lacking in the literature. The present article aims to give a comprehensive picture of the BG-based implants that have been developed in the context of ocular surgery; the strengths and shortcomings of the existing devices are outlined in order to provide useful stimuli for future research. Promising research directions are also proposed, emphasizing the added values that BGs could carry in ophthalmology in the light of recent findings in tissue engineering and regenerative medicine.

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Materials characterization and mechanobiology of the eye

Cited by 11 publications

References 158 publications

Nonswelling, Ultralow Content Inverse Electron‐Demand Diels–Alder Hyaluronan Hydrogels with Tunable Gelation Time: Synthesis and In Vitro Evaluation

Nonswelling, Ultralow Content Inverse Electron‐Demand Diels–Alder Hyaluronan Hydrogels with Tunable Gelation Time: Synthesis and In Vitro Evaluation

Biomechanical relationships between the corneal endothelium and Descemet's membrane

How can bioactive glasses be useful in ocular surgery?

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