“…Recent investigations have described the use of ESEM and its application in dentistry (21,22). The hydration of cements for biomaterial characterization might be also investigated in situ and in real time by micro-Raman spectroscopy, with no sample manipulation and minimal interference from environmental water (7,23).…”
“…Recent investigations have described the use of ESEM and its application in dentistry (21,22). The hydration of cements for biomaterial characterization might be also investigated in situ and in real time by micro-Raman spectroscopy, with no sample manipulation and minimal interference from environmental water (7,23).…”
“…This mechanism has not been widely exploited but gives rise to intrinsic contrast not available in a conventional SEM when a metallic coating has been applied. 8,[31][32][33][34] Figure 3 shows an image of an unstained block copolymer. The contrast between the lamellar phases is clear.…”
Section: Applications To Polymeric Systemsmentioning
Europe for unraveling the physics of messy materials ranging from cement to starch. This article is based, in part, on the topic area for which she received the award.Soft matter-also known as complex fl uids-is a fi eld of growing interest and importance, spanning many classes of materials, including polymers, biopolymers, colloids, and liquid crystals. Different approaches for microstructural characterization are more appropriate than those used for hard (and usually fully crystallized) materials such as metals and inorganic materials because of the time and length scales involved. This article discusses a range of techniques applicable to the characterization of soft matter, including environmental scanning electron microscopy (SEM) and microrheology. The former offers two key advantages for this class of material over conventional SEM because it requires neither a high vacuumwhich is a problem for hydrated samples-nor that an insulator be coated with a conductive material. Microrheology is well suited to small volumes of fl uid with low moduli that may be heterogeneous; it is capable of measuring gelation in real time.
“…e ESEM's ability to generate three-dimensional data from biological material surfaces in their "natural" condition (without the need to stain and fix) and to change environmental variables, has opened up new areas of use for material sciences with a focus on medicine. [7] us, the purpose of this study was to evaluate the amount of root repair taking place at varying intervals of 1 day, 2 weeks, 4 weeks, and 8 weeks after damage due to contact with microscrews. e results were evaluated by an environmental scanning electron microscope study (E-SEM).…”
Objective:
The purpose of this study was to evaluate the amount of root repair that took place at varying intervals of 1 day, 2 weeks, 4 weeks and 8 weeks after intentional contact with microscrews. The results were evaluated by an environmental scanning electron microscope study (E-SEM).
Material and Methods:
Ten orthodontic patients with 2 maxillary and 2 mandibular first premolars (40 premolars) to be extracted as part of the orthodontic treatment were included in this study (age 18–25 years). The position of the roots of the 4 premolars to be extracted was clinically determined and under local anesthesia, microscrew implants were placed in such a way that they directly hit the root from the buccal aspect. Implants were removed immediately after the above-mentioned procedure was performed. On the same day, premolar of the 1st quadrant (Group I) was extracted. Premolar of the 2nd quadrant (Group II) was extracted 2 weeks later. Premolar of the 3rd quadrant (Group III) was extracted 4 weeks later and premolar of the 4th quadrant (Group IV) was extracted 8 weeks later, from the day of intentional damage.
Results:
Significant changes were observed in the deposition of cementum over 8 weeks which were analyzed using ESEM under magnifications of × 50, × 200, × 500, and × 5000.
Conclusion:
The damaged root surfaces due to intentional contact with microscrews showed swift repair and healing within 8 weeks. In case, the root is damaged due to improper placement technique or wrong biomechanics which may result in the implant touching the root surface, a minimum healing period of 8–10 weeks is advocated before commencing further tooth movement.
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