Material‐mediated cell immobilization technology in the biological fermentation proces

Abstract

Cell immobilization technology has shown much potential in the field of biological fermentation owing to its simple operation, maintenance of long‐term cell viability, repeatability, good stability, and high tolerance. Compared with free‐cell fermentation, immobilized cells show strong growth and metabolism performance in complex environments, such as environments with low pH, or with high concentrations of substrate and product. The metabolic properties of immobilized cells mainly depend on the carrier materials. In recent years, polymer membrane materials have attracted much attention because of their good mechanical properties and flexible support structure. In addition to improving the activity and stability of immobilized cells, biocompatibility modification can also be carried out on the surface of the carrier, which can reduce specific non‐biological interactions between the cells and the carrier. In this review, current advances in cell immobilization using different materials in the field of biological fermentation are reviewed. Perspectives on immobilized cells and the challenges associated with them in the field of biological fermentation are also addressed. © 2021 Society of Chemical Industry and John Wiley & Sons, Ltd

Immobilization: the promising technique to protect and increase the efficiency of microorganisms to remove contaminants

“…38 These techniques provide physical confinement while allowing cells to retain their metabolic activity. 39 The choice of immobilization method depends on the specific application and desired characteristics of the immobilized cells. 40 Various techniques have been documented for the immobilization of cells, and they can be categorized into two main groups: chemical immobilization and physical immobilization, as illustrated in Fig.…”

“…Immobilized cells offer advantages in preparative fermentation processes, particularly in terms of productivity and operational flexibility. 39 The microenvironments provided by the carrier enhance enzyme activity and promote faster growth, leading to improved operational stability and productivity compared to free cells. 97 Immobilized cell systems can be effectively described using established theoretical and hydrodynamical treatments for heterogeneous catalysis, especially when solid carriers in the Figure 12.…”

“…Cell immobilization encompasses various techniques that immobilize living cells within a matrix or support material 38 . These techniques provide physical confinement while allowing cells to retain their metabolic activity 39 . The choice of immobilization method depends on the specific application and desired characteristics of the immobilized cells 40 .…”

Hierarchical Emulsion-Templated Monoliths (polyHIPEs) as Scaffolds for Covalent Immobilization of P. acidilactici

“…This IMCF system can be desirable over freely suspended cells since immobilization normally promotes an improvement in metabolic performance, cell stability, and product separation during fermentation, as well as permitting repeated catalysis for adherent cells [9,10]. In IMCF, it is crucial to establish an applicable carrier or scaffold that facilitates cell growth and the metabolism process since the surface chemistry of the immobilized carrier material can affect the activity and stability of immobilized cells [11,12]. Diverse carriers have recently been developed for cell immobilization to achieve excellent mass transfer and long-term cell viability.…”

Cell Immobilization for Erythritol Production

“…On the other hand, cell immobilization techniques have been applied successfully to other bioprocesses, which offers advantages in terms of its stability, and it allows for the reuse of the biocatalyst [24][25][26]. Cell immobilization consists of keeping a microorganism inside or on the surface of a supporting material in the long term in such a way that it can grow and reproduce normally while it interacts with the medium and performs its expected metabolic activities of industrial interest.…”