2021
DOI: 10.3390/ijms222112017
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MATE-Type Proteins Are Responsible for Isoflavone Transportation and Accumulation in Soybean Seeds

Abstract: Soybeans are nutritionally important as human food and animal feed. Apart from the macronutrients such as proteins and oils, soybeans are also high in health-beneficial secondary metabolites and are uniquely enriched in isoflavones among food crops. Isoflavone biosynthesis has been relatively well characterized, but the mechanism of their transportation in soybean cells is largely unknown. Using the yeast model, we showed that GmMATE1 and GmMATE2 promoted the accumulation of isoflavones, mainly in the aglycone… Show more

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Cited by 14 publications
(18 citation statements)
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“…The poly-glutamate motif of GmMATE4 was converted from EEEEEEE to AAAEEEE (GmMATE4∆3ala) or from EEEEEEE to AAAAAAA (GmMATE4∆7ala) with the use of specially designed primers. Then, the DNA fragments were cloned into the vector pGBKT7∆BD [7]. The primers and restriction enzymes used are listed in Table S1.…”
Section: Yeast Isoflavone Uptake Assaymentioning
confidence: 99%
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“…The poly-glutamate motif of GmMATE4 was converted from EEEEEEE to AAAEEEE (GmMATE4∆3ala) or from EEEEEEE to AAAAAAA (GmMATE4∆7ala) with the use of specially designed primers. Then, the DNA fragments were cloned into the vector pGBKT7∆BD [7]. The primers and restriction enzymes used are listed in Table S1.…”
Section: Yeast Isoflavone Uptake Assaymentioning
confidence: 99%
“…The yeast isoflavone uptake assays were performed, as described previously [7,25] with slight modifications. Untransformed Y2HGold was compared to GmMATE4-transformed yeast to rule out that the uptakes of isoflavones in GmMATE4 expressing yeast cells were solely due to the endogenous yeast proteins.…”
Section: Yeast Isoflavone Uptake Assaymentioning
confidence: 99%
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