Mastitis, polyarthritis and abortion caused by Mycoplasma species bovine group 7 in dairy cattle

Hum, S.; Kessell, Allan; Djordjevic, Steven P.; Rheinberger, R; Hornitzky, M.; Forbes, Wendy Ann; Gonsalves, Jocelyn R.

doi:10.1111/j.1751-0813.2000.tb10444.x

Cited by 38 publications

(50 citation statements)

References 15 publications

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“…Although the role of H 2 O 2 in the pathogenesis of Mycoplasma sp. bovine group 7 infections is unclear, field strains isolated during a recent outbreak of polyarthritis, mastitis and abortion were able to haemolyse red blood cells when cultured on sheep blood agar (Hum et al, 2000). These observations suggest that the release of H 2 O 2 by Mycoplasma sp.…”

Section: Discussionmentioning

confidence: 63%

“…Twenty-four isolates of Mycoplasma sp. bovine group 7 were recovered from multiple joint fluids, pericardial fluid and a lymph node of affected calves, from individual and bulk milk samples, and from the internal organs (liver, spleen, lung and stomach contents) of two aborted foetuses (Hum et al, 2000). Fingerprinting studies showed that all 24 isolates were indistinguishable from one another but could be readily differentiated from a collection of epidemiologically unrelated Australian and international isolates of this species (Djordjevic et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

“…bovine group 7 representative of the clonal cluster recovered from multiple animals and different tissue sites during an outbreak lysed sheep red blood cells when cultured on blood agar medium (Hum et al, 2000). The aim of this study was to identify the putative genes for glycerol transport (gtsABC), lppB, and the flanking segment containing ORF5 and ORF6 on the chromosome of PG50, and compare them with homologues in M. mycoides subsp.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, Mycoplasma sp. bovine group 7 was isolated during a severe outbreak of polyarthritis, mastitis and abortion affecting about 120 cattle in a large dairy operation in New South Wales, Australia (Hum et al, 2000). Twenty-four isolates of Mycoplasma sp.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of a chromosomal region of Mycoplasma sp. bovine group 7 strain PG50 encoding a glycerol transport locus (gtsABC)

2003

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Mycoplasma species bovine group 7, represented by the type strain PG50, is one of six members of the Mycoplasma mycoides cluster and has been implicated in sporadic and outbreak cases of polyarthritis and mastitis in Australian dairy cattle. This study describes cloning and sequencing a 7?9 kb region of the PG50 chromosome and identification of genes involved in glycerol transport (gtsA, gtsB and gtsC) that are followed by a putative lipoprotein gene lppB and a genomic locus containing two ORFs encoding putative membrane proteins. Long range PCR using primers spanning gtsABC and downstream flanking genes, and Southern hybridization analyses using a suite of probes derived from M. mycoides subsp. mycoides small colony type (SC) strain Afadé for gtsA, gtsB and gtsC, lppB and the two downstream genes confirmed that these genes were conserved among Mycoplasma sp. bovine group 7 isolates and mycoplasmas belonging to the M. mycoides subcluster [M. mycoides subsp. mycoides SC, M. mycoides subsp. mycoides large colony type (LC) and M. mycoides subsp. capri ] but were absent in mycoplasmas belonging to the Mycoplasma capricolum subcluster (M. capricolum subsp. capricolum and M. capricolum subsp. capripneumoniae). M. capricolum subsp. capricolum type strain California kid did not hybridize with the probe for gtsA and gave only weak or no hybridization signals with probes derived from the loci downstream of gtsABC, suggesting that this region has diverged in mycoplasmas belonging to subspecies of M. capricolum. It is shown that PG50, after the addition of a physiological concentration of glycerol to the growth medium, generates H 2 O 2 at levels comparable with strain Afadé , implying that the glycerol transport system is functional in Mycoplasma sp. bovine group 7. This suggests that in PG50, as in M. mycoides subsp. mycoides SC, glycerol uptake is followed by phosphorylation to glycerol 3-phosphate and then conversion to dihydroxyacetone phosphate, catalysed by L-a-glycerophosphate oxidase, resulting in the production of H 2 O 2 . The ability of Mycoplasma sp. bovine group 7 to generate significant amounts of hydrogen peroxide may be important in pathogenesis.

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Section: Discussionmentioning

confidence: 63%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of a chromosomal region of Mycoplasma sp. bovine group 7 strain PG50 encoding a glycerol transport locus (gtsABC)

2003

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“…MmmSC is responsible for contagious bovine pleuropneumonia (CBPP), which is considered to be the most important threat to cattle farming in affected countries, and one which impacts farmers directly through mortality and poor productivity of their animals. M. leachii is associated with polyarthritis in calves (12,23,24) and with mastitis (6), pneumonia (2), and abortion (12) in cows. Whereas, three M. mycoides cluster members, Mccp, Mmc, and Mcc, are the caprine pathogens.…”

Section: Introductionmentioning

confidence: 99%

Isolation and identification of Mycoplasma mycoides cluster strains from goats in Chongqing, China

Wang

Yang

et al. 2014

Bulletin of the Veterinary Institute in Pulawy

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In order to evaluate the prevalence of the Mycoplasma mycoides cluster in goats in Chongqing, China, an epidemiological survey in this area was carried out. A total of 68 samples were subjected to bacteria isolation on Hartley's medium. Four isolates (three from lung tissue and one from nasal discharges) were recovered from the samples and identified as the Mycoplasma species by their morphological and biochemical characteristics. They were further confirmed by PCR using 16S rRNA specific primer pairs and by restriction enzyme analysis. In vitro antimicrobial susceptibility of the isolates indicated that some strains had developed resistance to the antibiotics tested. This is the first report on the isolation, identification, and molecular characterisation of Mycoplasma species isolated from goats in Chongqing. This study also revealed a prevalence of Mycoplasma species infection in goats in this area.

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Genetic diversity amongMycoplasmaspecies bovine group 7: Clonal isolates from an outbreak of polyarthritis, mastitis, and abortion in dairy cattle

et al. 2001

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A comprehensive genetic analysis of 60 Mycoplasma sp. bovine group 7 isolates from different geographic origins and epidemiological settings is presented. Twenty-four isolates were recovered from the joints of calves during sporadic episodes of polyarthritis in geographically distinct regions of Queensland and New South Wales, Australia, including two clones of the type strain PG5O. A further three Australian isolates were also recovered from the tympanic bulla, retropharyngeal lymph node and the lung and another three isolates had unconfirmed histories. Six isolates originated from Germany, Portugal, Nigeria, and France. Twenty-four epidemiologically related isolates of Mycoplasma sp. bovine group 7 were recovered from multiple tissue sites and body fluids of infected calves with polyarthritis, mastitic milk, and from the stomach contents, lung and liver from aborted foetuses in three large, centrally managed dairy herds in New South Wales, Australia. Restriction endonuclease analysis (REA) of genomic DNA differentiated 29 Cfol profiles among these 60 isolates and grouped all 24 epidemiologically related isolates in a defined pattern showing a clonal origin. Three isolates of this clonal cluster were recovered from mastitic milk and the synovial exudate of clinically-affected calves and appeared sporadically for periods up to 18 months after the initial outbreak of polyarthritis indicating a persistent, close association of the organism with cattle in these herds. The Cfol profile representative of the clonal cluster was distinguishable from profiles of isolates recovered from multiple, unrelated cases of polyarthritis in Queensland and New South Wales and from other countries. All 24 isolates from the clonal cluster possessed a plasmid (pBG7AU) with a molecular size of 1022 bp. DNA sequence analysis of pBG7AU identified two open reading frames sharing 81 and 99% DNA sequence similarity with hypothetical replication control proteins A and B respectively, previously described in plasmid pADB201 isolated from M. mycoides subspecies mycoides. Other isolates of bovine group 7, epidemiologically unrelated to the clonal cluster, including two clones of the type strain PG5O, possessed a similar-sized plasmid. These data confirm that Mycoplasma sp. bovine group 7 is capable of migrating to, and multiplying within, different tissue sites within a single animal and among different animals within a herd.

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Mastitis, polyarthritis and abortion caused by Mycoplasma species bovine group 7 in dairy cattle

Cited by 38 publications

References 15 publications

Characterization of a chromosomal region of Mycoplasma sp. bovine group 7 strain PG50 encoding a glycerol transport locus (gtsABC)

Characterization of a chromosomal region of Mycoplasma sp. bovine group 7 strain PG50 encoding a glycerol transport locus (gtsABC)

Isolation and identification of Mycoplasma mycoides cluster strains from goats in Chongqing, China

Genetic diversity amongMycoplasmaspecies bovine group 7: Clonal isolates from an outbreak of polyarthritis, mastitis, and abortion in dairy cattle

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