Mastitis in dairy buffalo and cattle in Egypt due to Clostridium perfringens: prevalence, incidence, risk factors and costs

Osman, Kamelia M.; El-Enbaawy, Mona I.; Ezzeldeen, Nashwa A.; Hussein, Hassan

doi:10.20506/rst.28.3.1936

Cited by 23 publications

(18 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There were differences observed between rumen fluid and feces, and also within fecal samples. The major bacterial pathogens identified from smallholder farms in this study, which included Salmonella enterica, Klebsiella pneumoniae, Streptococcus sciuri, Campylobacter jejuni, and Staphylococcus aureus, among others were in agreement with the findings reported by Osman et al (2009) and Sharif and Muhammad (2009).…”

Section: Discussionsupporting

confidence: 92%

Metagenomic analysis of enteric bacterial pathogens affecting the performance of dairy cows in smallholder productions systems

Habimana¹,

Bett²,

Amimo³

et al. 2018

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

There is little information about the diversity of bacterial pathogens present in the rumen and feces of healthy cow and the subsequent effects on the performance of the host animal. The objectives of the present study were to genetically characterize the enteric bacterial pathogens found in the rumen fluid and cow feces and to identify the resistant genes responsible for antimicrobial resistance in the detected pathogens. The cow feces and rumen fluid samples (6 rumen fluid and 42 feces) were collected from lactating dairy cows. Using next generation sequencing, the enteric bacterial pathogens detected were screened for antimicrobial resistance genes using ResFinder-2.1 database in the center of Abricate. The characterized enteric bacterial pathogens include Escherichia coli, Salmonella enterica, Streptococcus agalactiae, Streptococcus pyogenes, Campylobacter coli, and Campylobacter fetus among others. Those enteric bacterial pathogens were also drug resistant bacteria except Campylobacter coli. The Campylobacter fetus fetus was identified as the only multidrug resistant bacterial pathogen detected in the cow feces. However, the abundant resistant genes detected confer resistance to tetracycline (17 genes from 209 contigs), beta-lactam (21 genes from 67 contigs), streptomycin (6 genes from 153 contigs), and sulfamethoxazole (2 genes from 72 contigs). This is the first study to identify the diversity of enteric bacterial pathogens from the station based and smallholder dairy cows in Kenya and Tanzania, respectively.

show abstract

Section: Discussionsupporting

confidence: 92%

Metagenomic analysis of enteric bacterial pathogens affecting the performance of dairy cows in smallholder productions systems

Habimana¹,

Bett²,

Amimo³

et al. 2018

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

show abstract

“…Clostridium perfringens was isolated from 3/50 (6%) milk samples. Other findings were reported by Osman et al (2009) at which C. perfringens isolated from 16/375 (4.48%) of milk samples from cows and 1/25 (4.0%) of samples from buffalo, but (Amer and El-Mossalami, 2006) could not detect C. perfringens in any of the examined milk samples. Clostridium perfringens could be isolated from 4/50 (8%) kareish cheese samples.…”

Section: Discussionmentioning

confidence: 66%

Bacteriological and molecular studies on toxigenic Clostridium perfringens in milk and some milk products

Tawab

Ammar

Aideia

et al. 2016

Benha Veterinary Medical Journal

View full text Add to dashboard Cite

Two hundred random samples of milk, kareish cheese, yoghurt and ice-cream (50 for each) were examined microbiologically for the presence of Clostridium perfringens, their enterotoxigencity and their antibiotic sensitivity. Clostridium perfringens was isolated from 3 (6%) milk samples, 4 (8%) kareish cheese samples and it could not be isolated from any examined samples of yoghurt and ice-cream. The majority of C. perfringens isolates recovered from milk and milk products were susceptible to ofloxacin, ampicillin + sulbactam and norfloxacin (100%), vancomycin, tetracycline, metronidazole and amoxicillin + clavulinic acid (83.3%) and clindamycin (66.7%). The majority were resistant to cephalothin (100%), sulphamethoxazole + trimethoprim (83.3%), oxacillin and chloramphenicol (66.7%). Molecular studies using multiplex PCR technique for detection of alpha toxin gene and C.perfringens types "A" enterotoxin gene revealed that the 7 isolates of C. perfringens (100%) were positive for alpha toxin gene and only 2 out of 7 isolates (28.57%) were positive for enterotoxin gene .

show abstract

“…Similarly, in this study, C. butyricum and C. sporogenes were also found to be abundant in winter than in summer and were representatives from botulinum Groups I and II. From a food safety perspective, C. perfringens is considered significant because of the ability of some strains to induce illness (Grass, Gould, & Mahon, ) and have also been recurrently identified as the causative agents of mastitis in bovine and other ruminants (Osman, El‐Enbaawy, Ezzeldeen, & Hessein, ). C. perfringens have been isolated from Australian dairy farms, specifically from milk filters, feces and soil (McAuley et al., in ).…”

Section: Discussionmentioning

confidence: 99%

“…and have also been recurrently identified as the causative agents of mastitis in bovine and other ruminants(Osman, El-Enbaawy, Ezzeldeen, & Hessein, 2009). C. perfringens have been isolated from Australian dairy farms, specifically from milk filters, feces and soil(McAuley et al, in 2014).…”

mentioning

confidence: 99%

Farm level survey of spore‐forming bacteria on four dairy farms in the Waikato region of New Zealand

Gupta

Brightwell

2017

MicrobiologyOpen

View full text Add to dashboard Cite

The aim of our study was to determine the occurrence and diversity of economically important spore‐forming bacteria in New Zealand dairy farm systems. Farm dairy effluent (FDE) collected from Waikato dairy farms were tested for the presence of spore‐forming bacteria, using a new culture‐based methodology followed by genomic analysis. An enrichment step in which samples were inoculated in cooked meat glucose starch broth under anaerobic conditions, aided in the differential isolation of Bacillus and Clostridium species. Furthermore, the use of molecular methods such as ERIC genotyping, 16S rRNA gene sequence analysis identified different spore‐forming bacteria present in FDE. C. sporogenes signature PCR gave further information on the phylogenetic relationship of the different Clostridium spp. isolated in this study. In total 19 Bacillus spp., 5 Paenibacillus spp. and 17 Clostridium spp. were isolated from farm dairy effluent. Sequence types similar to economically important food spoilage bacteria viz: C. butyricum, C. sporogenes and members of the Paenibacillus Genus were isolated from all four farms, whereas, sequence types similar to potential toxigenic, B. cereus, C. perfringens, C. butyricum, and C. botulinum were found on at least three of the farms. Sampling of farm dairy effluent provides a good indicator of farm level prevalence of bacterial load as it is used to irrigate dairy pasture in New Zealand. This study highlights the presence of various spore‐forming bacteria in dairy waste water and indicates the implementation of good hygienic farm practices and dairy waste effluent management.

show abstract

Mastitis in dairy buffalo and cattle in Egypt due to Clostridium perfringens: prevalence, incidence, risk factors and costs

Cited by 23 publications

References 17 publications

Metagenomic analysis of enteric bacterial pathogens affecting the performance of dairy cows in smallholder productions systems

Metagenomic analysis of enteric bacterial pathogens affecting the performance of dairy cows in smallholder productions systems

Bacteriological and molecular studies on toxigenic Clostridium perfringens in milk and some milk products

Farm level survey of spore‐forming bacteria on four dairy farms in the Waikato region of New Zealand

Contact Info

Product

Resources

About