Master athletes have higher miR-7, SIRT3 and SOD2 expression in skeletal muscle than age-matched sedentary controls

Koltai, Erika; Bori, Zoltán; Osváth, Péter; Ihász, Ferenc; Stella, Péter; Tóth, Gábor; Degens, Hans; Rittweger, Jörn; Boldogh, István; Radák, Zsolt

doi:10.1016/j.redox.2018.07.022

Cited by 46 publications

(45 citation statements)

References 47 publications

(50 reference statements)

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“…We have not been able to enroll rugby player women, but previous studies have reported benefices of physical activity in the cognitive health of aged women (Yaffe et al, 2001;Lin et al, 2019). Furthermore, as discussed above, old female master athletes have shown higher mRNA and protein levels of SIRT1 and SIRT3 in skeletal muscle than aged-matched controls (Koltai et al, 2018) suggesting activation of SIRT1-SIRT3 axis. Therefore, no differential gender response is anticipated.…”

Section: Discussionmentioning

confidence: 90%

“…The significantly increased physical activity of the player group compared to that of the age-matched controls may be the cause of their rejuvenation-like gene expression in blood cells. Similarly, male and female master athletes aged 60-70 years have shown higher mRNA and protein levels of SIRT1 and SIRT3 in skeletal muscle than aged-matched controls (Koltai et al, 2018). It is plausible that the levels of sirtuins in veteran sport practitioners, both track and field athletes, and rugby players, were also higher in the brain.…”

Section: Discussionmentioning

confidence: 97%

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Peripheral Maintenance of the Axis SIRT1-SIRT3 at Youth Level May Contribute to Brain Resilience in Middle-Aged Amateur Rugby Players

Corpas

Solana

Rosa

et al. 2019

Front. Aging Neurosci.

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Physical exercise performed regularly is known to improve health and to reduce the risk of age-related diseases. Furthermore, there is some evidence of cognitive improvement in physically active middle-aged and older adults. We hypothesized that long-term physically active middle-aged men may have developed brain resilience that can be detected with the analysis of peripheral blood markers. We aimed to analyze the activation of pathways potentially modulated by physical activity in a cohort of healthy amateur rugby players (n = 24) and control subjects with low physical activity (n = 25) aged 45-65 years. We had previously reported neuropsychological improvement in immediate memory responses in the player group compared to the controls. Here, we tested the expression of selected genes of longevity, inflammation, redox homeostasis, and trophic signaling in whole blood mRNA. Analyses were also performed on blood samples of young (aged 15-25 years) control subjects with low physical activity (n = 21). Physical activity and other lifestyle factors were thoroughly recorded with standardized questionnaires. Interestingly, middle-aged control subjects showed lower levels of expression of SIRT1, SIRT3, CAT, and SOD1 than the young controls, although rugby players maintained the expression levels of these genes at a young-like level. Middle-aged players showed lower levels of IL1B than the non-physically active groups. However, there was a tendency towards a decrease in trophic and transduction factors in middle-aged groups as compared to the young controls. A statistical study of Spearman's correlations supported a positive effect of sporting activity on memory

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 97%

Peripheral Maintenance of the Axis SIRT1-SIRT3 at Youth Level May Contribute to Brain Resilience in Middle-Aged Amateur Rugby Players

Corpas

Solana

Rosa

et al. 2019

Front. Aging Neurosci.

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“…The knockdown of miR-7 is shown to induce myoblast differentiation [29]. Also, miR-7 is downregulated in skeletal muscle of master athletes [85]. An increase in the expression of miR-7 levels has been recognized as a therapeutic target for opposing muscle dysfunction in DM1 [86].…”

Section: Discussionmentioning

confidence: 99%

CDR1as related miRNA-mRNA networks in differentiating goat skeletal muscle satellite cells

Kyei

Liu

et al. 2020

Preprint

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Background: Myogenesis is a complex process controlled by several coding and non-coding RNAs (ncRNAs) such as circular RNAs (circRNAs) that well-known function as endogenous microRNAs (miRNAs) sponges. Over the past few years, numerous circRNAs have been known and their roles in biological processes have begun to be understood. Cerebellar Degeneration-Related protein 1 antisense (CDR1as), the most spotlighted circRNA as miR-7 sponge that has been blooming circRNAs’ research for a decade, and can potentially sponge several miRNAs in disease and muscle physiology. Nevertheless, the linear-RNAs-differed character that the acute interventions for circRNAs do not affect miRNAs levels, and has retarded the transcriptome-wide discovery of miRNAs sponged by. Therefore, the purpose of this study was to provide the transcriptomic effect of CDR1as during muscle differentiation.Methods: siCDR1as and siDICER1 were transfected into goat skeletal muscle satellite cells (SMSCs). RNA-seq technology and bioinformatics tools were used to analyze genes that are deregulated by siCDR1as and siDICER1. quantitative PCR was used to verify the expression levels of the differentially expressed mRNAs and miRNAs. Results: Here, to systematically identify miRNAs targeting CDR1as, we employed the critical enzyme DICER1 that governs the biogenesis of miRNAs. The deficiency of either DICER1 or CDR1as inhibited myogenic differentiation of SMSCs, and knockdown of DICER1 decreased the expression of CDR1as. Moreover, we screened for the targeted messenger RNAs (mRNAs) and miRNAs in SMSCs transfected with siDICER1 or siCDR1as respectively and found out that some well-known muscle-related pathways such as phosphoinositide 3-kinase (PI3K)-AKT signaling pathway, Rap1 signaling pathway, and MAPK signaling pathway were enriched in all groups. Further, regarding the miRNAs identified in siDICER1 and siCDR1as together with the sequence complementary information, we identified 11 miRNAs including miR-1, miR-206, and miR-27a-5p which are more likely to be novel targets for CDR1as. Conclusion: In summary, our study provides a perspective on the potential functions and relationship between CDR1as and DICER1 during muscle development.

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“…On the one hand, ALS onsets occur typically after age 50 and manifest partially through muscle weakness. Also, PSMD13 is linked to aging [47] and high expression of the gene has been found in skeletal muscle of athletes [48], suggesting that under-expression could be a sign of muscle weakness. These results validate the relevance of our framework for de novo disease-gene association prediction.…”

Section: Predicting Disease-gene Associationsmentioning

confidence: 99%

Unveiling new disease, pathway, and gene associations via multi-scale neural network

et al. 2020

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Diseases involve complex modifications to the cellular machinery. The gene expression profile of the affected cells contains characteristic patterns linked to a disease. Hence, new biological knowledge about a disease can be extracted from these profiles, improving our ability to diagnose and assess disease risks. This knowledge can be used for drug re-purposing, or by physicians to evaluate a patient's condition and co-morbidity risk.Here, we consider differential gene expressions obtained by microarray technology for patients diagnosed with various diseases. Based on these data and cellular multi-scale organization, we aim at uncovering disease-disease links, disease-gene and disease-pathways associations. We propose neural networks with structures based on the multi-scale organization of proteins in a cell into protein complexes and pathways. We show that these models are able to correctly predict the diagnosis for the majority of patients. Through the analysis of the trained models, we predict disease-disease, disease-pathway, and disease-gene associations and validate the predictions by comparisons to known interactions and literature search, proposing putative explanations for the predictions.

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Master athletes have higher miR-7, SIRT3 and SOD2 expression in skeletal muscle than age-matched sedentary controls

Cited by 46 publications

References 47 publications

Peripheral Maintenance of the Axis SIRT1-SIRT3 at Youth Level May Contribute to Brain Resilience in Middle-Aged Amateur Rugby Players

Peripheral Maintenance of the Axis SIRT1-SIRT3 at Youth Level May Contribute to Brain Resilience in Middle-Aged Amateur Rugby Players

CDR1as related miRNA-mRNA networks in differentiating goat skeletal muscle satellite cells

Unveiling new disease, pathway, and gene associations via multi-scale neural network

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