Mast cells and eosinophilic granule cells in Oncorhynchus mykiss: Are they similar or different?

Romano, Luís Alberto; Oliveira, Fernando Pablo Silva; Pedrosa, Virgínia Fonseca

doi:10.1016/j.fsirep.2021.100029

Cited by 4 publications

(2 citation statements)

References 37 publications

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“…Histological section stained with May-Grunwald Giemsa revealed the presence of cytoplasmic granule-rich MCs, distributed in the lamina propria and also in intraepithelial position (Figure 2b), according to Romano et al (2021). AB/PAS has shown the presence of numerous goblet cells in the intestinal epithelium.…”

Section: Resultsmentioning

confidence: 98%

Mast cells in goldfish (Carassius auratus) gut: Immunohistochemical characterization

et al. 2022

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The common goldfish is the most widespread teleosts in the world. Due to its peculiar characteristics, such as the high resistance, easy availability and stabulation, and for its evolutionary characteristics, this fish lends itself to be one of the most used experimental models. This study aimed to characterize the mast cells in the intestine of Carassius auratus using anti‐TLR‐2, anti‐S100, anti‐VIP, anti‐serotonin (5‐HT) and anti‐Piscidin antibodies. The intestine of goldfish, like that of all vertebrates, plays an important role in the immunology of the animal. The gut‐associated lymphoid tissue GALT is an immune component containing several specific cells such as lymphocytes, macrophages and mast cells. In addition, the presence of goblet cells in the intestinal epithelium strengthens the defence system, secreting many cytokines and chemokines and displaying antibacterial properties. Our results show mast cells labelled with antibodies that are highly conserved between fish and mammals, demonstrating an active role of these cells in the immune response.

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Section: Resultsmentioning

confidence: 98%

Mast cells in goldfish (Carassius auratus) gut: Immunohistochemical characterization

et al. 2022

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“…If we could perform immunohistochemical staining for mast cell markers, it would be interesting for us to provide detailed information on similarities and differences between mammalian mast cells and fish EGCs. Because a more recent study (Romano et al, 2021) of Oncorhynchus mykiss tissues including M. ÖZTOP 4963 intestines reports that mast cells and EGCs are representative of well-differentiated cell populations due to differences in their staining affinities although they have some common characteristics.…”

Section: Discussionmentioning

confidence: 99%

Density, Distribution and Staining Properties of Eosinophilic Granular Cells in Oscar Fish (Astronotus ocellatus) Intestine

Öztop¹

2023

J Hellenic Vet Med Soc

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Fish eosinophilic granular cells are found in the connective tissues of structures associated with the external environment. These cells are similar to mammalian mast cells in terms of structural and functional characteristics. The cytoplasmic granules of these cells give different staining reactions depending on fixative type. This study aimed to determine the staining properties and densities of eosinophilic granular cells in Oscar fish (Astronotus ocellatus Agassiz, 1831) intestine using different fixatives and histochemical techniques. Formalin and basic lead acetate fixation-giemsa staining indicated that eosinophilic granular cells were abundant in anterior intestine, localizing around especially the blood vessels and submucosa. Giemsa staining of Bouin’s fixed rather than other fixatives showed that eosinophilic granular cells were higher in posterior intestine. No reaction was observed in eosinophilic granular cells in Thionin and Toluidine Blue staining in any fixative. Eosinophilic granular cells in samples fixed with different fixatives had metachromatic Alcian Blue staining. In conclusion, this study shows that fixatives may have different effects on the distribution and staining properties of eosinophilic granular cells in Oscar fish intestinal regions. Abundance in anterior intestine and around submucosal blood vessels shows that eosinophilic granular cells could play an active role in mucosal immunity against food-borne pathogens.

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Expression of Antimicrobic Peptide Piscidin1 in Gills Mast Cells of Giant Mudskipper Periophthalmodon schlosseri (Pallas, 1770)

Alesci

Capillo

Mokhtar

et al. 2022

IJMS

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The amphibious teleost Giant mudskipper (Periophthalmodon schlosseri, Pallas 1770) inhabit muddy plains and Asian mangrove forests. It spends more than 90% of its life outside of the water, using its skin, gills, and buccal-pharyngeal cavity mucosa to breathe in oxygen from the surrounding air. All vertebrates have been found to have mast cells (MCs), which are part of the innate immune system. These cells are mostly found in the mucous membranes of the organs that come in contact with the outside environment. According to their morphology, MCs have distinctive cytoplasmic granules that are released during the degranulation process. Additionally, these cells have antimicrobial peptides (AMPs) that fight a variety of infections. Piscidins, hepcidins, defensins, cathelicidins, and histonic peptides are examples of fish AMPs. Confocal microscopy was used in this study to assess Piscidin1 expression in Giant Mudskipper branchial MCs. Our results demonstrated the presence of MCs in the gills is highly positive for Piscidin1. Additionally, colocalized MCs labeled with TLR2/5-HT and Piscidin1/5-HT supported our data. The expression of Piscidin1 in giant mudskipper MCs highlights the involvement of this peptide in the orchestration of teleost immunity, advancing the knowledge of the defense system of this fish.

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Mast cells and eosinophilic granule cells in Oncorhynchus mykiss: Are they similar or different?

Cited by 4 publications

References 37 publications

Mast cells in goldfish (Carassius auratus) gut: Immunohistochemical characterization

Mast cells in goldfish (Carassius auratus) gut: Immunohistochemical characterization

Density, Distribution and Staining Properties of Eosinophilic Granular Cells in Oscar Fish (Astronotus ocellatus) Intestine

Expression of Antimicrobic Peptide Piscidin1 in Gills Mast Cells of Giant Mudskipper Periophthalmodon schlosseri (Pallas, 1770)

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