2005
DOI: 10.1074/jbc.m506338200
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Mast Cell Tryptase Controls Paracellular Permeability of the Intestine

Abstract: cleavage. When applied to the basolateral surface of colonocytes, PAR 2 agonists and mast cell supernatant decreased transepithelial resistance, increased transepithelial flux of macromolecules, and induced redistribution of tight junction ZO-1 and occludin and perijunctional F-actin. When mast cells were co-cultured with colonocytes, mast cell degranulation increased paracellular permeability of colonocytes. This was prevented by a tryptase inhibitor. We determined the role of ERK1/2 and of ␤-arrestins, which… Show more

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Cited by 287 publications
(258 citation statements)
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References 46 publications
(77 reference statements)
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“…63,64 Furthermore, PAR2 may induce the activation of extracellular signal-related kinase 1/2 (ERK1/2) and phosphorylation of myosin light chain kinase, which regulates reorganization of F-actin and cytoskeleton and redistribution of tight junction, to increase epithelial permeability. 63,65 Other MC mediators such as interferon-γ, tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-4, IL-13, and prostaglandin E2 also have destructive effects on both trans-and paracellular permeability. 65 More recently, further investigations were carried out to map the regulation of permeability in follicle-associated epithelium (FAE), which is a specialized epithelium that covers Peyer's patches, and contain microfold cells that are dedicated to sampling antigens and bacteria from the lumen and transporting them to the underlying immune aggregate in the intestinal mucosa.…”
Section: Mast Cells Modulate Epithelial Permeability and Mucosal Barriermentioning
confidence: 99%
See 2 more Smart Citations
“…63,64 Furthermore, PAR2 may induce the activation of extracellular signal-related kinase 1/2 (ERK1/2) and phosphorylation of myosin light chain kinase, which regulates reorganization of F-actin and cytoskeleton and redistribution of tight junction, to increase epithelial permeability. 63,65 Other MC mediators such as interferon-γ, tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-4, IL-13, and prostaglandin E2 also have destructive effects on both trans-and paracellular permeability. 65 More recently, further investigations were carried out to map the regulation of permeability in follicle-associated epithelium (FAE), which is a specialized epithelium that covers Peyer's patches, and contain microfold cells that are dedicated to sampling antigens and bacteria from the lumen and transporting them to the underlying immune aggregate in the intestinal mucosa.…”
Section: Mast Cells Modulate Epithelial Permeability and Mucosal Barriermentioning
confidence: 99%
“…61,62 Numerous evidence showed a positive relationship between the number of mucosal MCs and intestinal permeability, 61 and the MC-derived tryptase was well identified as a key factor disrupts the intestinal barrier. 63 MC tryptase cleaves PAR2 on colonocytes to increase paracellular permeability by acting on intercellular apical junction complex, which mainly consists of the tight junctions such as claudins, occludin, zonula occludens, junctional adhesion molecule, and the adherens junction such as E-cadherin. 63,64 Furthermore, PAR2 may induce the activation of extracellular signal-related kinase 1/2 (ERK1/2) and phosphorylation of myosin light chain kinase, which regulates reorganization of F-actin and cytoskeleton and redistribution of tight junction, to increase epithelial permeability.…”
Section: Mast Cells Modulate Epithelial Permeability and Mucosal Barriermentioning
confidence: 99%
See 1 more Smart Citation
“…On the other hand, the tryptase released by mast cells upon activation has been shown to induce TJs disassembly through the activation of proteinase-activated receptor-2 of the epithelial cells. [88][89][90] These receptors can modulate enteric neurotransmission, secretion, motility, epithelial permeability, and visceral sensitivity, and are also known to regulate intestinal inflammation. 91 Anatomical contacts between mast cells and enteric nerve fibers have been demonstrated in the human gastrointestinal mucosa and inflammation multiplies these contacts.…”
Section: Permeabilitymentioning
confidence: 99%
“…Mast cells have been shown by a number of groups to modify the epithelial barrier function within the intestine and, when activated, to increase intestinal permeability and fluid loss. [53][54][55][56][57] Such processes could explain enhanced weight loss in mast cell-containing animals.…”
Section: Discussionmentioning
confidence: 99%