Mast Cell Recovery following Chronic Treatment with Compound 48/80

Abstract

Histochemical and functional properties of mast cells (MC) in Brown Norway rats recovering from chronic treatment with the MC secretagogue compound 48/ 80 were examined. In the skin, treatment for 5 days with compound 48/80 resulted in a marked decrease in MC subpopulations defined by differential alcian blue/safranin staining. Both safranin-positive connective tissue MC and alcian blue staining MC were reduced in number. This was accompanied by significant decreases in skin histamine and rat MC serine protease I contents and a loss of specific IgE-mediated passive cutaneous anaphylaxis (PCA) activity. The PCA reaction did not return to normal before 2 months after stopping treatment and only when the numbers of safranin-positive connective tissue MC and skin histamine content reached pretreatment levels. The subepidermal alcian blue staining MC not eliminated by the compound 48/80 treatment were formalin resistant (unlike alcian blue staining mucosal MC of the intestine) and apparently played no role in the PCA response. MC numbers, histamine levels, and rat MC serine protease 1 content of the tongue were similarly decreased by compound 48/80. In contrast, mucosal MC of the gut were unaffected by the secretagogue treatment.

Brain mast cell degranulation regulates blood-brain barrier

“…We observed a significant decrease in toluidine blue-positive MH mast cell number in C48/80 treated birds, as previously reported in mammalian and avian species (Ibrahim, 1970;Jaffery et al, 1994;Ribatti et al, 1992). This was expected because upon C48 /80 treatment, mast cells lose their granular material and the glycosaminoglycans contained therein.…”

Involvement of Mast Cells in Adenosine-Mediated Bronchoconstriction and Inflammation in an Allergic Mouse Model

“…Chronic treatment with C48/80 has been shown to deplete mast cells of their granular contents (Jaffery et al, 1994;Karadag et al, 2000). Adenosine is involved in asthma through its ability to promote or enhance mediator release from mast cells (Peachell et al, 1988;Jacobson and Bai, 1997).…”

Inhibition of mast cell-dependent immediate-type hypersensitivity reactions by purple bamboo salt

“…Jaffery et al (1994) reported that the number of mast cells in skin decreased by chronic treatment with compound 48/80 by differential alcian blue/safranin staining. There is a possibility that the mast cells cannot be detected by alcian blue because of the complete degranulation by compound 48/80.…”