Mast Cell Proteoglycans

Rönnberg, Elin; Melo, Fabio Rabelo; Pejler, Gunnar

doi:10.1369/0022155412458927

Cited by 114 publications

(81 citation statements)

References 103 publications

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“…Such a stimulation might be comparable to that induced by soluble anti-IgE. As b-hexosaminidase is not associated with the serglycin matrix 42 , it immediately diffuses in the extracellular medium independently of the stimulation condition. As a consequence, the amount of b-hexosaminidase measured in the cell supernatant is similar following both localized and non-polarized stimulation.…”

Section: Discussionmentioning

confidence: 99%

Mast cells form antibody-dependent degranulatory synapse for dedicated secretion and defence

et al. 2015

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Mast cells are tissue-resident immune cells that play a key role in inflammation and allergy. Here we show that interaction of mast cells with antibody-targeted cells induces the polarized exocytosis of their granules resulting in a sustained exposure of effector enzymes, such as tryptase and chymase, at the cell-cell contact site. This previously unidentified mast cell effector mechanism, which we name the antibody-dependent degranulatory synapse (ADDS), is triggered by both IgE-and IgG-targeted cells. ADDSs take place within an area of cortical actin cytoskeleton clearance in the absence of microtubule organizing centre and Golgi apparatus repositioning towards the stimulating cell. Remarkably, IgG-mediated degranulatory synapses also occur upon contact with opsonized Toxoplasma gondii tachyzoites resulting in tryptase-dependent parasite death. Our results broaden current views of mast cell degranulation by revealing that human mast cells form degranulatory synapses with antibody-targeted cells and pathogens for dedicated secretion and defence.

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Section: Discussionmentioning

confidence: 99%

Mast cells form antibody-dependent degranulatory synapse for dedicated secretion and defence

et al. 2015

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“…Since toluidine blue is a cationic dye, it exhibits the capacity to selectively integrate with the glycosaminoglycan-protein complex (highly ionized) 11 . A hallmark feature of mast cells from all species is their striking metachromatic staining with various cationic dyes such as toluidine.…”

Section: Discussionmentioning

confidence: 99%

Analysis of the presence and location of mast cells in periapical cysts and periapical granulomas

Nogueira

Farias

Silva

et al. 2016

RGO, Rev. Gaúch. Odontol.

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Objective The aim of the present study was to locate mast cells in chronic periapical lesions (granulomas and cysts) by using histochemical techniques and toluidine blue staining. Methods RESUMO ObjetivoLocalizar mastócitos em lesões periapicais (granulomas e cistos) através de técnicas histoquímicas e corante azul de toluidina. MétodosUma pesquisa quantitativa, descritiva, transversal e retrospectiva foi realizada. As amostras foram obtidas de documentos histopatológicos nos arquivos do laboratório de patologia cirúrgica da Universidade de Pernambuco entre Novembro de 2014 e Março de 2015. ResultadosDezesseis casos de granuloma e 21 casos de cistos periapicales foram selecionados. As lâminas coradas foram analisadas por dois examinadores em tempos diferentes, em um estudo duplo-cego. Mastócitos foram encontrados em 13 (61,9%) dos casos de cistos periapicales, localizados na cápsula da lesão. Nos granulomas periapicais, mastócitos foram encontrados em oito casos (50%), localizados no tecido de granulação. ConclusãoMastócitos foram detectados tanto em cistos quanto em granulomas periapicais, localizados na cápsula e no tecido de granulação respectivamente. Os mastócitos estavam mais presentes nos cistos do que nos granulomas periapicais.

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“…A role for proteoglycans in the homeostasis and storage of proteases and amines in secretory granules has been well studied in mast cells (Rönnberg et al 2012, Wernersson & Pejler 2014. For the exocrine pancreas, however, studies showing the presence of proteoglycans in ZGs have so far been descriptive only.…”

Section: Discussionmentioning

confidence: 99%

“…The latter enclose sulfated proteoglycans, which have been identified in ZG and in the fluid of the pancreatic ductal system (Berg & Young 1971, Reggio & Palade 1978, Tartakoff et al 1975. The negatively charged proteoglycans in secretory granules of hematopoietic cells and mast cells are involved in the binding of small positively charged molecules, such as amines and proteases, and have therefore been considered to promote the efficient storage of secretory products (Rönnberg et al 2012, Wernersson & Pejler 2014. Similarly, proteoglycans within ZGs have been proposed to support ZG biogenesis and to concentrate at the ZG membrane (Kleene et al 1999, Scheele et al 1994, Schmidt et al 2000, Schrader 2004), but their role in ZG formation is unclear.…”

Section: The Formation Of Zg In the Acinar Cells Is Initiated At The mentioning

confidence: 99%

Proteoglycans support proper granule formation in pancreatic acinar cells

Aroso

Agricola

Hacker

et al. 2015

Histochem Cell Biol

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Zymogen granules (ZG) are specialized organelles in the exocrine pancreas which allow digestive enzyme storage and regulated secretion. The molecular mechanisms of their biogenesis and the sorting of zymogens are still incompletely understood. Here, we investigated the role of proteoglycans in granule formation and secretion of zymogens in pancreatic AR42J cells, an acinar model system. Cupromeronic Blue cytochemistry and biochemical studies revealed an association of proteoglycans primarily with the granule membrane. Removal of proteoglycans by carbonate treatment led to a loss of membrane curvature indicating a supportive role in the maintenance of membrane shape and stability.Chemical inhibition of proteoglycan synthesis impaired the formation of normal electrondense granules in AR42J cells and resulted in the formation of unusually small granule structures. These structures still contained the zymogen carboxypeptidase, a cargo molecule of secretory granules, but migrated to lighter fractions after density gradient centrifugation.Furthermore, the basal secretion of amylase was increased in AR42J cells after inhibitor treatment. In addition, irregular-shaped granules appeared in pancreatic lobules. We conclude that the assembly of a proteoglycan scaffold at the ZG membrane is supporting efficient packaging of zymogens and the proper formation of stimulus-competent storage granules in acinar cells of the pancreas.Aroso et al.

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Mast Cell Proteoglycans

Cited by 114 publications

References 103 publications

Mast cells form antibody-dependent degranulatory synapse for dedicated secretion and defence

Mast cells form antibody-dependent degranulatory synapse for dedicated secretion and defence

Analysis of the presence and location of mast cells in periapical cysts and periapical granulomas

Proteoglycans support proper granule formation in pancreatic acinar cells

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