Massive Prevalence of Faecal Carriage of ESBL-Producing  &lt;i&gt;Escherichia Coli&lt;/i&gt; in Community in Niger Due to the Spread of  &lt;i&gt;blac&lt;/i&gt; &lt;sub&gt;CTX-M-15&lt;/sub&gt; Gene in Multiple Commensal Clones

Jacquier, Hervé; Assao, Bachir; Chau, Françoise; Guindo, Ousmane; Condamine, Bénédicte; Magnan, Mélanie; Bridier‐Nahmias, Antoine; Sayingoza-Makombe, Nathan; Moumouni, Aissatou; Page, Anne‐Laure; Langendorf, Céline; Coldiron, Matthew E.; Denamur, Erick; Lastours, Victoire de

doi:10.2139/ssrn.4099407

Cited by 2 publications

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“…24 Feglo and Adu-Sarkodie in 2016 (57.8%, n = 234/405), 25 28 It is worth noting that variations in study populations can also influence ESBL carriage rates. For example, the proportion of ESBL faecal carriage reported in our work is lower than that cited for similar populations in comparative reviews 6,17,18 spanning several regions across Africa, including Uganda in 2023 (61%), 29 Ghana in 2022 (53.1%), 30 Kenya in 2022 (44%), 31 Niger in 2022 (92.4%), 32 Malawi in 2021 (47.2%), 33 Central African Republic in 2016 (59%), 10 Morocco in 2014 (42.9%), 14 and Cameroon in 2013 (55.3%). 11 In all these studies, the reports were silent on whether the study patients were immunocompetent or immunosuppressed.…”

Section: Discussioncontrasting

confidence: 72%

Role of CTX-M-15 gene in spread of extended-spectrum beta-lactamases among immunocompetent patients in Ghana

Obeng-Nkrumah,

Tawiah-Abrokwa,

Owusu

et al. 2023

African Journal of Laboratory Medicine

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Background: Patients with faecal carriage of extended-spectrum beta-lactamases (ESBL)-producing Enterobacterales serve as reservoirs and sources of dissemination and infection.Objective: This report examined immunocompetent patients for faecal carriage of ESBL-producing Enterobacterales in a district care hospital setting in Ghana.Methods: Between March 2019 and May 2020, cross-sectional sampling was performed to enrol patients and conduct questionnaire-structured interviews for factors that predispose patients to ESBL faecal carriage. Faecal samples from study patients were quantified for ESBL-producing Enterobacterales. The ESBL genes were characterised by polymerase chain reaction and sequencing.Results: The overall proportion of ESBL faecal carriage was 35.5% (n = 38/107). The blaCTX-M gene, mostly CTX-M-15, was detected in 89.5% (n = 34/38) of the ESBL-producing isolates. The other ESBL types included blaSHV (n = 3) and blaOXA (n = 1). The CTX-M-15-positive isolates, when present in a faecal sample compared to the non-ESBL-CTX-M-15 isolates, constituted the predominant faecal Enterobacterales, with significantly higher colony counts than all other enterobacteria in that sample. In multivariate regression, independent risk factors for faecal carriage of ESBL-producing Enterobacterales were hospitalisation in the past year, infections since admission, use of antibiotics in the past 6 weeks, and admission from another hospital.Conclusion: The study found that CTX-M-15-producing isolates were the predominant faecal Enterobacterales, and that further investigations are needed to determine the reasons behind this dominance.What this study adds: The CTX-M-15-producing isolates dominance in this study shows the misuse and abuse of antibiotics in an African medical facility and indicates the potential role of immunity in controlling ESBL spread, which is to be investigated further.

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Section: Discussioncontrasting

confidence: 72%

Role of CTX-M-15 gene in spread of extended-spectrum beta-lactamases among immunocompetent patients in Ghana

Obeng-Nkrumah,

Tawiah-Abrokwa,

Owusu

et al. 2023

African Journal of Laboratory Medicine

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“…Based on E. coli typing results, it is important to mention that most of the identified E. coli serotypes were already found to be associated with human diseases, such as the O92:H33 serotype isolated from stool samples of patients examined in the frame of clinical indications [66]; O111:H21 associated to a household outbreak in Northern Ireland [67]; two E. coli O45:H2 associated to outbreaks in the United States that have caused 18 cases of illness [68]; O125ac:H6 isolated from cases of diarrhea in Brazil, Germany and Australia [69]; O55:H10 already associated to cases of Hemolytic Uremic Syndrome (HUS) [70] and E. coli O25:H4-ST131 isolated from urine culture of a patient undergoing chronic hemodialysis at a tertiary care center in Chennai, India, in 2014 [71]. O105:H7 and ESBL O101:H9 were also already detected in human stool samples [72,73].…”

Section: Discussionmentioning

confidence: 99%

Genotypic and Phenotypic Characterization of Pathogenic Escherichia coli, Salmonella spp., and Campylobacter spp., in Free-Living Birds in Mainland Portugal

Batista

Saraiva²,

Lopes³

et al. 2022

IJERPH

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Birds are potential carriers of pathogens affecting humans and agriculture. Aiming to evaluate the occurrence of the top three most important foodborne pathogens in free-living birds in Portugal, we investigated 108 individual fecal samples from free-living birds and one pooled sample of gull feces (n = 50) for the presence of Escherichia coli (pathogenic and non-pathogenic), Salmonella spp. and Campylobacter spp. Virulence- and antimicrobial resistance- (AMR) associated genes were detected by PCR and Whole-Genome Sequencing (WGS), and phenotypic (serotyping and AMR profiles) characterization was performed. Overall, 8.9% of samples tested positive for pathogenic E. coli, 2.8% for Salmonella spp., and 9.9% for Campylobacter spp. AMR was performed on all pathogenic isolates and in a fraction of non-pathogenic E. coli, being detected in 25.9% of them. Ten of the tested E. coli isolates were multidrug-resistant (MDR), and seven of them were Extended-spectrum β-lactamase (ESBL) producers. Among Salmonella (n = 3) and Campylobacter (n = 9), only one strain of C. jejuni was identified as MDR. Most of the identified serotypes/sequence types had already been found to be associated with human disease. These results show that free-living birds in Portugal may act as carriers of foodborne pathogens linked to human disease, some of them resistant to critically important antimicrobials.

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Massive Prevalence of Faecal Carriage of ESBL-Producing <i>Escherichia Coli</i> in Community in Niger Due to the Spread of <i>blac</i> <sub>CTX-M-15</sub> Gene in Multiple Commensal Clones

Cited by 2 publications

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Role of CTX-M-15 gene in spread of extended-spectrum beta-lactamases among immunocompetent patients in Ghana

Role of CTX-M-15 gene in spread of extended-spectrum beta-lactamases among immunocompetent patients in Ghana

Genotypic and Phenotypic Characterization of Pathogenic Escherichia coli, Salmonella spp., and Campylobacter spp., in Free-Living Birds in Mainland Portugal

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