Massive phenotyping of multiple cranberry populations reveals novel QTLs for fruit anthocyanin content and other important chemical traits

Diaz‐Garcia, Luis; Schlautman, Brandon; Covarrubias‐Pazaran, Giovanny; Maule, Andrew J.; Johnson‐Cicalese, Jennifer; Grygleski, Edward; Vorsa, Nicholi; Zalapa, Juan

doi:10.1007/s00438-018-1464-z

Cited by 29 publications

(29 citation statements)

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“…Genomic resources (mapping populations) have been recently used to map QTL for development and fruit quality traits in other berry crops, like raspberry [19], blackberry [20], cranberry [21][22][23][24] and strawberry [25]. The first genetic linkage maps for red raspberry were generated from a 'Glen Moy' × 'Latham' population [26,27].…”

Section: Relationship To the Big Picturementioning

confidence: 99%

“…Fruit quality traits related to fruit development, texture, color, sugar, anthocyanin and organic acid contents have been mapped in strawberry as well [25]. In cranberry, which is a member of the Vaccinium genus and is closely related to blueberry, QTL for fruit rot resistance [21,22], total anthocyanin content, titratable acidity, proanthocyanidin content, Brix and mean fruit weight [23], and fruit shape and size [24] have all been identified.…”

Section: Relationship To the Big Picturementioning

confidence: 99%

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Phenotypic Evaluation of a Hybrid Diploid Blueberry Population for Plant Development and Fruit Quality Traits

2020

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A diploid blueberry mapping population, used previously to map quantitative trait loci (QTL) for chilling requirement and cold hardiness, was evaluated for several plant development and fruit quality traits. Specifically, the population was phenotyped in a greenhouse for timing of various stages of flower bud, leaf bud and fruit development and for fruit quality traits including weight, diameter, color, scar, firmness, flavor and soluble solids. Phenotypic data was analyzed statistically by analysis of variance, correlation tests, to examine associations of traits, and heritability. Results indicated that the traits were segregating and most were distributed normally in the population. Many of the development traits were correlated, and timing of shoot expansion, early bloom and full bloom was also correlated with the previously evaluated trait of chilling requirement. Some correlations were found among the fruit quality traits as well. For example, weight was highly correlated with diameter, and subjectively measured firmness was moderately correlated with one of the objectively measured firmness traits. In addition, most of the traits showed significant variation across genotypes and across years, and most had moderate to high heritability. Therefore, we conclude that the diploid population should be useful for identifying QTL for many of these traits.

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Section: Relationship To the Big Picturementioning

confidence: 99%

Section: Relationship To the Big Picturementioning

confidence: 99%

Phenotypic Evaluation of a Hybrid Diploid Blueberry Population for Plant Development and Fruit Quality Traits

2020

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“…In 2013, the first simple sequence repeat (SSR)-based genetic map was published, in which several quantitative trait loci (QTL) for yield-related traits and important phytochemicals were reported [21]. Subsequently, high-density genetic maps as well as more multi-trait QTL studies became available [22,23,24,25,26,27]. In addition, nuclear [28], plastid [29], and mitochondrial [30] genomes have been published recently.…”

Section: Introductionmentioning

confidence: 99%

Pacbio Sequencing Reveals Identical Organelle Genomes between American Cranberry (Vaccinium macrocarpon Ait.) and a Wild Relative

Diaz‐Garcia

Rodríguez-Bonilla

Rohde

et al. 2019

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Breeding efforts in the American cranberry (Vaccinium macrocarpon Ait.), a North American perennial fruit crop of great importance, have been hampered by the limited genetic and phenotypic variability observed among cultivars and experimental materials. Most of the cultivars commercially used by cranberry growers today were derived from a few wild accessions bred in the 1950s. In different crops, wild germplasm has been used as an important genetic resource to incorporate novel traits and increase the phenotypic diversity of breeding materials. Vaccinium microcarpum (Turcz. ex Rupr.) Schmalh. and V. oxycoccos L., two closely related species, may be cross-compatible with the American cranberry, and could be useful to improve fruit quality such as phytochemical content. Furthermore, given their northern distribution, they could also help develop cold hardy cultivars. Although these species have previously been analyzed in diversity studies, genomic characterization and comparative studies are still lacking. In this study, we sequenced and assembled the organelle genomes of the cultivated American cranberry and its wild relative, V. microcarpum. PacBio sequencing technology allowed us to assemble both mitochondrial and plastid genomes at very high coverage and in a single circular scaffold. A comparative analysis revealed that the mitochondrial genome sequences were identical between both species and that the plastids presented only two synonymous single nucleotide polymorphisms (SNPs). Moreover, the Illumina resequencing of additional accessions of V. microcarpum and V. oxycoccos revealed high genetic variation in both species. Based on these results, we provided a hypothesis involving the extension and dynamics of the last glaciation period in North America, and how this could have shaped the distribution and dispersal of V. microcarpum. Finally, we provided important data regarding the polyploid origin of V. oxycoccos.

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“…Fruit size, shape, weight and color are among some of the parameters widely used by researchers, breeders, and the industry. Several phytochemicals, mainly those that confer certain characteristics such as flavor or nutritional value, complete the list of fruit quality traits often considered in cranberry [37]. However, it is undeniable that fruit firmness and the internal characteristics of fruit play an important role in both industry processing and consumers’ preference.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive analysis of the internal structure and firmness in American cranberry (Vaccinium macrocarpon Ait.) fruit

et al. 2019

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BackgroundCranberry (Vaccinium macrocarpon L.) fruit quality traits encompass many properties. Although visual appearance and fruit nutritional constitution have usually been the most important attributes, cranberry textural properties such as firmness have recently gained importance in the industry. Fruit firmness has become a quality standard due to the recent demand increase for sweetened and dried cranberries (SDC), which are currently the most profitable cranberry product. Traditionally, this trait has been measured by the cranberry industry using compression tests; however, it is poorly understood how fruit firmness is influenced by other characteristics.ResultsIn this study, we developed a high-throughput computer-vision method to measure the internal structure of cranberry fruit, which may in turn influence cranberry fruit firmness. We measured the internal structure of 16 cranberry cultivars measured over a 40-day period, representing more than 3000 individual fruit evaluated for 10 different traits. The internal structure data paired with fruit firmness values at each evaluation period allowed us to explore the correlations between firmness and internal morphological characteristics.ConclusionsOur study highlights the potential use of internal structure and firmness data as a decision-making tool for cranberry processing, especially to determine optimal harvest times and ensure high quality fruit. In particular, this study introduces novel methods to define key parameters of cranberry fruit that have not been characterized in cranberry yet. This project will aid in the future evaluation of cranberry cultivars for in SDC production.

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Massive phenotyping of multiple cranberry populations reveals novel QTLs for fruit anthocyanin content and other important chemical traits

Cited by 29 publications

References 51 publications

Phenotypic Evaluation of a Hybrid Diploid Blueberry Population for Plant Development and Fruit Quality Traits

Phenotypic Evaluation of a Hybrid Diploid Blueberry Population for Plant Development and Fruit Quality Traits

Pacbio Sequencing Reveals Identical Organelle Genomes between American Cranberry (Vaccinium macrocarpon Ait.) and a Wild Relative

Comprehensive analysis of the internal structure and firmness in American cranberry (Vaccinium macrocarpon Ait.) fruit

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