Massilia cellulosiltytica sp. nov., a novel cellulose-degrading bacterium isolated from rhizosphere soil of rice (Oryza sativa L.) and its whole genome analysis

Abstract

A bacterial strain, Gram-stain negative, rod-shaped, aerobic and cellulose-degrading, designated NEAU-DD11 T , was isolated from rhizosphere soil of rice collected from Northeast Agricultural University in Harbin, Heilongjiang province, North-east China. Base on 16S rRNA gene sequence analysis, strain NEAU-DD11 T belongs to the genus Massilia and shared high sequence similarities with Massilia phosphatilytica 12-OD1 T (98.46 %) and Massilia putida 6NM-7 T (98.41 %). Phylogenetic analysis based on the 16S rRNA gene and whole genome sequences indicated that strain NEAU-DD11 T formed a stable cluster with M. phosphatilytica 12-OD1 T and M. putida 6NM-7 T . The major fatty acids of the strain were C 16:0 , C 17:0 -cyclo and C 16:1 ω7c. The respiratory quinone was Q-8. The polar lipids pro le of the strain showed the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidenti ed polar lipid and an unidenti ed phospholipid. In addition, the digital DNA-DNA hybridization values between strain NEAU-DD11 T and M. phosphatilytica 12-OD1 T and M. putida 6NM-7 T were 45.4% and 35.6%, respectively, which are lower than the accepted threshold value of 70%. The DNA G + C content of strain NEAU-DD11 T was 66.2 %. The whole genome analysis showed the strain contained carbohydrate enzymes such as glycoside hydrolase and polysaccharide lyase, which enabled the strain to had the function of degrading cellulose. On the basis of the phenotypic, genotypic and chemotaxonomic characteristics, strain NEAU-DD11 T represents a novel species of the genus Massilia, for which the name Massilia cellulosiltytica sp. nov. is proposed. The type strain is NEAU-DD11 T (= CCTCC AB 2019141 T = DSM 109721 T ).

Genome-wide and constrained ordination-based analyses of EC code data support reclassification of the species of Massilia La Scola et al. 2000 into Telluria Bowman et al. 1993, Mokoshia gen. nov. and Zemynaea gen. nov.

“…The species ‘ Massilia cellulosiltytica ’, the name of which is misspelled in the original description [28], ‘ Massilia aromaticivorans ’ [29] and ‘ Massilia antibiotica ’ [30] are all distinct and well-described species despite not having achieved validation. Since the type strains have adequate etymology and culture collection depositions, these species are reclassified here as Telluria cellulosilytica sp.…”

“…The species 'Massilia cellulosiltytica' , the name of which is misspelled in the original description [28], 'Massilia aromaticivorans' [29] and 'Massilia antibiotica' [30] are all distinct and well-described species despite not having achieved validation. Since the type Fig.…”

“…The description is the same as given by Du et al . [28] where this species was given as ‘ Massilia cellulosiltytica ’. Type strain is NEAU DD11 T =CCTCC AB 2019141 T =DSM 109721 T .…”

Bioplastic (poly-3-hydroxybutyrate)-producing Massilia endophytica sp. nov., isolated from Cannabis sativa L. ‘Cheungsam’

“…As of now, the genus Massilia comprises 61 species with valid and correct names. Massilia is a Gram-negative, rod-shaped aerobic bacteria that have relatively high DNA G + C content (63.3-66.3 mol%) [5][6][7][8][9][10][11][12][13] . The major fatty acids in this genus are summed feature 3 (C 16:1 ω7c and/or C 16:1 ω6c) and C 16:0, while the major respiratory quinone is Q-8, and the major polar lipids are phosphatidylethanolamine (PE), phosphatidylglycerol (PG), and diphosphatidylglycerol (DPG) 14 .…”

“…Massilia species possess diverse potential functions, such as the production of violacein 15,16 , degradation of benzene, toluene, ethylbenzene, and xylene (BTEX) 11 , solubilization of phosphate 17 , production of dimethyl disul de 18 , adaptation to cold environments 19 , degradation of cellulose 7 , antibacterial activity 8 , degradation of chloroacetamide herbicide 20 , and accumulation of poly-3hydroxybutyrate (PHB) 21 .…”