Mass transport behavior of electrochemical species through plasma-polymerized thin film on platinum electrode

Hiratsuka, Atsunori; Muguruma, Hitoshi; Nagata, Ryohei; Nakamura, Runa; Sato, Kimiharu; Uchiyama, Sadao; Karube, Isao

doi:10.1016/s0376-7388(00)00403-8

Cited by 41 publications

(36 citation statements)

References 29 publications

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“…The characteristics of PPFs and their applications in biosensor development have been summarized by Muguruma and Karube [117] and by Hiratsuka and Karube [118], and have been described in an original research article [119]. PPFs are formed under dry, vacuum conditions.…”

Section: Plasma Polymerized Films (Ppfs)mentioning

confidence: 99%

Current research activity in biosensors

Nakamura

Karube

2003

Analytical and Bioanalytical Chemistry

259

129

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Biosensors consist of a molecular recognition element and a transducer. Since the first biosensor was developed by Updike and Hicks many biosensors and their associated techniques have been studied and developed. In this review current research activity on the fundamentals and applications of biosensors is summarized. In discussion of the former, molecular recognition elements, techniques and tools for biosensor construction, and basic biosensor devices are introduced. Coverage of the latter includes description of biosensors for environmental, food, and clinical fields. Chemical sensors developed in our laboratory for environmental monitoring are also described. This review mainly summarizes work performed by our group and by our colleagues, and refers to the main review articles summarizing each field of biosensor research in recent years.

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Section: Plasma Polymerized Films (Ppfs)mentioning

confidence: 99%

Current research activity in biosensors

Nakamura

Karube

2003

Analytical and Bioanalytical Chemistry

259

129

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“…On the other hand, polymers such as nafion or polyurethane may produce an additional, unstable, interfacial potential which varies depending upon the ionic environment [23]. Furthermore, the spin-and dipcoating methods typically used for depositing nafion on electrode surfaces are difficult to control so as to produce thin, homogeneous, reproducible, and strongly adhesive films [24]. The gradual three-step baking of polyimide at elevated temperatures is an important factor in formation of the final rigid insulation material.…”

Section: Chip Designmentioning

confidence: 99%

Native and sodium dodecyl sulfate‐capillary gel electrophoresis of proteins on a single microchip

et al. 2004

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Simultaneous electrophoresis of both native and Sodium dodecyl sulfate (SDS) proteins was observed on a single microchip within 20 min. The capillary array prevented lateral diffusion of SDS components and avoided cross contamination of native protein samples. The planar sputtered electrode format provided a more uniform distribution of separation voltage into each of the 36 parallel microchannel capillaries than platinum wire electrodes commonly used in conventional electrophoresis. The customized geometry of the stacking capillary machined into the cover plate of the microchip facilitated reproducible sample injection without the requirement for stacking gel. Polyimide served as a mask and facilitated insulation of the anode and cathode to prevent electrode lift off and deterioration during continuous electrophoresis, even at a constant current of 8 mA. Improved protein separation was observed during capillary electrophoresis at lower currents. Ferguson plot analysis confirmed the electrophoretic mobility of native globular proteins in accordance with their charge and size. Corresponding Ferguson plot analysis of SDS-associated proteins on the same chip confirmed separation of marker proteins according to their molecular weight.

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“…The film contained an abundance of nitrogen atoms in the form of various functional groups, of which 32 percent were primary amines. 21 These amino groups facilitated the immobilization of GDH via covalent bonding with the bifunctional reagent glutaraldehyde. The substrate (glucose) and coenzyme (NAD) were simultaneously positioned in the vicinity of the active site of the enzyme (GDH), so that the GDH immobilized on the surface of the PPF retained its activity.…”

Section: Resultsmentioning

confidence: 99%

“…The surface of the acetonitrile PPF had a multitude of amino groups to which enzyme molecules could be immobilized. 21 Lastly, the enzyme was immobilized. Some 0.25% aqueous glutaraldehyde solution was added dropwise to the surface of the acetonitrile PPF, followed washing with water for 10 min.…”

Section: Device Fabricationmentioning

confidence: 99%