Mass Spectrometry Imaging (MSI) for Plant Metabolomics

Boughton, Berin A.; Thinagaran, Dinaiz

doi:10.1007/978-1-4939-7819-9_17

Cited by 25 publications

(15 citation statements)

References 14 publications

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“…At this stage the radicle and the coleoptile containing the primary leaves were fully emerged in cv BTx623, whereas in tcd1 that was delayed until 48 h AI. For each stage and line, three seeds (including roots and seedlings when present) were sectioned longitudinally at 10 mm thickness, placed on precooled glass slides, and immediately freeze-dried, using the method reported by Kawamoto and Kawamoto (2014), with the few modifications reported by Gorzolka et al (2016), Boughton and Thinagaran (2018), Sarabia et al (2018), and Schmidt et al (2018). Three consecutive sections were selected and subjected to respectively fluorescence microscopy and MSI analyses in both positive and negative mode to allow detection of an extensive range of metabolites.…”

Section: Spatial Distribution Of General and Specialized Metabolitesmentioning

confidence: 99%

Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging of Metabolites during Sorghum Germination

et al. 2020

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Dhurrin is the most abundant cyanogenic glucoside found in sorghum (Sorghum bicolor) where it plays a key role in chemical defense by releasing toxic hydrogen cyanide upon tissue disruption. Besides this well-established function, there is strong evidence that dhurrin plays additional roles, e.g. as a transport and storage form of nitrogen, released via endogenous recycling pathways. However, knowledge about how, when and why dhurrin is endogenously metabolized is limited. We combined targeted metabolite profiling with matrix-assisted laser desorption/ionization-mass spectrometry imaging to investigate accumulation of dhurrin, its recycling products and key general metabolites in four different sorghum lines during 72 h of grain imbibition, germination and early seedling development, as well as the spatial distribution of these metabolites in two of the lines. Little or no dhurrin or recycling products were present in the dry grain, but their de novo biosynthesis started immediately after water uptake. Dhurrin accumulation increased rapidly within the first 24 h in parallel with an increase in free amino acids, a key event in seed germination. The trajectories and final concentrations of dhurrin, the recycling products and free amino acids reached within the experimental period were dependent on genotype. Matrix-assisted laser desorption/ionization-mass spectrometry imaging demonstrated that dhurrin primarily accumulated in the germinating embryo, confirming its function in protecting the emerging tissue against herbivory. The dhurrin recycling products, however, were mainly located in the scutellum and/or pericarp/seed coat region, suggesting unknown key functions in germination.

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Section: Spatial Distribution Of General and Specialized Metabolitesmentioning

confidence: 99%

Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging of Metabolites during Sorghum Germination

et al. 2020

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“…However, at present, there is a growing interest in narrowing PSMs analyses down to the single-cell level, allowing the separation of plant cells from their potentially associated microbial cells. Such separations and individualized analyses can be achieved using approaches such as MSI ( Boughton and Thinagaran, 2018 ), matrix-assisted laser desorption ionization (MALDI) and laser ablation electrospray ionization (LAESI) ( Etalo et al, 2018a ; Bhattacharjee et al, 2020 ), live single-cell mass spectrometry (LSC-MS) ( Masuda et al, 2018 ), nanospray desorption electrospray ionization mass spectrometry (Nano-DESI MS) ( Battin et al, 2016 ), and the spatial metabolomics pipeline (metaFISH) ( Geier et al, 2020 ). In combination with MS data alignment and molecular networking software and relevant databases, these tools allow for the detection of a large number (hundreds to thousands) of metabolites acquired from a single plant cell ( Brader et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Linking Plant Secondary Metabolites and Plant Microbiomes: A Review

et al. 2021

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Plant secondary metabolites (PSMs) play many roles including defense against pathogens, pests, and herbivores; response to environmental stresses, and mediating organismal interactions. Similarly, plant microbiomes participate in many of the above-mentioned processes directly or indirectly by regulating plant metabolism. Studies have shown that plants can influence their microbiome by secreting various metabolites and, in turn, the microbiome may also impact the metabolome of the host plant. However, not much is known about the communications between the interacting partners to impact their phenotypic changes. In this article, we review the patterns and potential underlying mechanisms of interactions between PSMs and plant microbiomes. We describe the recent developments in analytical approaches and methods in this field. The applications of these new methods and approaches have increased our understanding of the relationships between PSMs and plant microbiomes. Though the current studies have primarily focused on model organisms, the methods and results obtained so far should help future studies of agriculturally important plants and facilitate the development of methods to manipulate PSMs–microbiome interactions with predictive outcomes for sustainable crop productions.

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“…It includes sample storage and cryosection. It is generally recommended to store samples at mild freezing conditions, such as wrapping samples with aluminum foil then put them in liquid nitrogen, rather than in direct contact with frozen medium [2,25]. For slice preparation, DESI generally does not require this procedure, while the sample preparation procedure of SIMS is similar to MALDI except that the former does not require matrix deposition.…”

Section: Plant Tissues Preparationmentioning

confidence: 99%

Mass spectrometry imaging for direct visualization of components in plants tissues

Han

Sheng

et al. 2021

J of Separation Science

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Mass spectrometry is considered the most informative technique for components identification and has been widely adopted in plant sciences. However, the spatial distribution of compounds in the plant, which is vital for the exploration of plant physiological mechanisms, is missed in MS analysis. In recent years, mass spectrometry imaging has brought a great breakthrough in plant analysis because it can determine both the molecular compositions and spatial distributions, which is conducive to understand functions and regulation pathways of specific components in plants. Mass spectrometry imaging analysis of plant tissue is toward high sensitivity, high spatial resolution, and even single-cell analysis. Despite many challenges and technical barriers, such as difficulties of sample pretreatment caused by morphological diversity of plant tissues, obstacles for high spatial resolution imaging, and so on, lots of researches have contributed to remarkable progress, including improvement in tissue preparation, matrix innovation, and ionization mode development. This review focuses on the advances of mass spectrometry imaging analysis of plants in the last 5 years, including commonly used ionization techniques, technical advances, and recent applications of mass spectrometry imaging in plants.

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Mass Spectrometry Imaging (MSI) for Plant Metabolomics

Cited by 25 publications

References 14 publications

Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging of Metabolites during Sorghum Germination

Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging of Metabolites during Sorghum Germination

Linking Plant Secondary Metabolites and Plant Microbiomes: A Review

Mass spectrometry imaging for direct visualization of components in plants tissues

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