Mass spectrometry imaging advances and application in pharmaceutical research

Abstract

Mass spectrometry imaging (MSI) has been shown to be a valuable tool through nearly every stage of the preclinical drug research and development (R&D) pipeline, and even to the early phase of clinical pharmaceutical evaluation. MSI can specifically resolve distributions of a parent drug and its metabolic products across dosed specimens without loss of spatial information, thus facilitating the direct observation of a drug’s pharmacokinetic processes, such as absorption, distribution, metabolism, and excretion. MSI can simultaneously visualize hundreds of phenotype molecules, including proteins, glycans, metabolites, and lipids, which have unique distribution patterns and biofunctions across different physiologic regions. This featured specificity in the chemical and physical spaces empowers MSI as an ideal analytical technique in exploring a drug’s pharmacodynamic properties, including in vitro/in vivo efficacy, safety, potential toxicity, and possible molecular mechanism. The application of MSI in pharmaceutical research has also been expanded from the conventional dosed tissue analysis to the front end of the preclinical drug R&D pipeline, such as investigating the structure-activity relationship, high-throughput in vitro screening, and ex vivo studies on single cells, organoids, or tumor spheroids. This review summarizes MSI application in pharmaceutical research accompanied by its technical and methodologic advances serving this central demand.

Mimotope peptide modified pompon mum-like magnetic microparticles for precise recognition, capture and biotransformation analysis of rituximab in biological fluids

“…S6 and S7 . These critical biotransformation should be carefully monitored during quality control, biosimilar development or treatment cycle to guarantee efficacy and safety 44 , 45 , 46 , 47 , 48 .

Figure 7 Analytical process for non-Hodgkin's lymphoma patient serum and example data of identified and confirmed peptides using ZenoTOF TM 7600 system.

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Microfluidic-based cardiovascular systems for advanced study of atherosclerosis

“…9e and f). 138,139,143 By combining microfluidics with microneedle patches and smart wearable devices, it may be possible to achieve dynamic monitoring of cardiovascular biomarkers. However, integrating microneedle patches and wearable devices with microfluidic technology for dynamic monitoring of cardiovascular diseases poses several challenges.…”

Recent developments and applications of ambient mass spectrometry imaging in pharmaceutical research: an overview

“…In addition to locating the abundance of drugs and metabolites, MSI, arguably among the most promising and evolving approaches to researching spatial multi-omics, also sheds light on the phenotypic alterations underpinning illness development and medication administration. 2–4 This study offers an analytical and methodological review of the use of ambient MSI in pharmacological research. Table 1 presents a list of the nature of the MSI application, drug type, sample, ambient ionization technique, MS analyzer, and supplementary analytical techniques.…”