“…In addition to locating the abundance of drugs and metabolites, MSI, arguably among the most promising and evolving approaches to researching spatial multi-omics, also sheds light on the phenotypic alterations underpinning illness development and medication administration. 2–4 This study offers an analytical and methodological review of the use of ambient MSI in pharmacological research. Table 1 presents a list of the nature of the MSI application, drug type, sample, ambient ionization technique, MS analyzer, and supplementary analytical techniques.…”