Mass spectrometry based targeted metabolomics precisely characterized new functional metabolites that regulate biofilm formation in Escherichia coli

Guo, Rui; Luo, Xialin; Liu, Jingjing; Lü, Haitao

doi:10.1016/j.aca.2020.12.021

Cited by 25 publications

(16 citation statements)

References 54 publications

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“…CPS biosynthesis requires iron (Zubaidah et al, 2014). Iron is vital for the metabolism of L-tryptophan, 5'-MTA, spermidine, CMP, and L-leucine, which is essential for forming the extracellular matrix, that is, exopolysaccharide (Guo et al, 2021). The above results show that AOEP effectively reduces cell-free CPS in KPESBL.…”

Section: Discussionmentioning

confidence: 70%

Secondary metabolite of Aspergillus oryzae impairs cell envelope integrity Klebsiella pneumoniae producing extended-spectrum beta-lactamase

Fitri¹,

Prawiro²,

Raras³

2023

J App Pharm Sc

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The defense system of Klebsiella pneumoniae producing extended-spectrum beta-lactamase (KPESBL) against the environment depends on the cell envelope and capsular polysaccharide (CPS). Its integrity depends on peptidoglycanassociated lipoprotein (pal), murein lipoprotein (lpp), and outer membrane protein A (ompA) covered by CPS. The outer membrane protects the cell envelope and serves as a siderophore entry-exit channel and membrane anchor for the CPS and fimbriae, crucial for K. pneumoniae pathogenicity. Here, we describe the effects of the secondary metabolite of Aspergillus oryzae (SMAO) on cell envelope integrity, cell adhesion, CPSs, siderophore, and KPESBL proliferation. The SMAO was exposed to the strain (KPESBL). Using quantitative real-time polymerase chain reaction, the expression of the pal, lpp, and ompA genes was assessed; CPS levels were measured using the Congo red binding assay (CR). Siderophore was measured using the chrome azurol sulphonate assay. Bacterial cell adherence was measured using the yeast agglutination assay. The growth curve was measured using the microdilution method. As validation, scanning electron microscopy was used to examine the structure of the cell membrane following exposure to SMAO. SMAO impairs the integrity of the cell envelope of KPESBL. Our research shows that SMAO may be a possible anti-infectious drug that breaks down the integrity of the cell envelope.

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Section: Discussionmentioning

confidence: 70%

Secondary metabolite of Aspergillus oryzae impairs cell envelope integrity Klebsiella pneumoniae producing extended-spectrum beta-lactamase

Fitri¹,

Prawiro²,

Raras³

2023

J App Pharm Sc

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“…In comparison, biofilms of E. coli UTI189 show decreases in 5′-MTA and 5’-CMP, as well increases in metal-binding siderophores, when Fe 3+ concentrations in culture decrease from 10 to 1 μM ( Guo et al, 2021 ). Lowered 5′-MTA abundances are associated with biofilms of Pseudomonas aeruginosa PAO1 ( Tielen et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Metabolomic and cultivation insights into the tolerance of the spacecraft-associated Acinetobacter toward Kleenol 30, a cleanroom floor detergent

Mogul

Miller²,

Ramos³

et al. 2023

Front. Microbiol.

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IntroductionStringent cleaning procedures during spacecraft assembly are critical to maintaining the integrity of life-detection missions. To ensure cleanliness, NASA spacecraft are assembled in cleanroom facilities, where floors are routinely cleansed with Kleenol 30 (K30), an alkaline detergent.MethodsThrough metabolomic and cultivation approaches, we show that cultures of spacecraft-associated Acinetobacter tolerate up to 1% v/v K30 and are fully inhibited at ≥2%; in comparison, NASA cleanrooms are cleansed with ~0.8-1.6% K30.ResultsFor A. johnsonii 2P08AA (isolated from a cleanroom floor), cultivations with 0.1% v/v K30 yield (1) no changes in cell density at late-log phase, (2) modest decreases in growth rate (~17%), (3) negligible lag phase times, (4) limited changes in the intracellular metabolome, and (5) increases in extracellular sugar acids, monosaccharides, organic acids, and fatty acids. For A. radioresistens 50v1 (isolated from a spacecraft surface), cultivations yield (1) ~50% survivals, (2) no changes in growth rate, (3) ~70% decreases in the lag phase time, (4) differential changes in intracellular amino acids, compatible solutes, nucleotide-related metabolites, dicarboxylic acids, and saturated fatty acids, and (5) substantial yet differential impacts to extracellular sugar acids, monosaccharides, and organic acids.DiscussionThese combined results suggest that (1) K30 manifests strain-dependent impacts on the intracellular metabolomes, cultivation kinetics, and survivals, (2) K30 influences extracellular trace element acquisition in both strains, and (3) K30 is better tolerated by the floor-associated strain. Hence, this work lends support towards the hypothesis that repeated cleansing during spacecraft assembly serve as selective pressures that promote tolerances towards the cleaning conditions.

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“…In the case of a biofilm, sampling usually begins at a washing step intended for removing growth media and loosely attached planktonic cells that could contribute with confounding signal ( Sadiq et al, 2020 ; Guo et al, 2021 ). In addition, biofilms have to be detached first due to their recalcitrant nature.…”

Section: Generation and Analysis Of Metabolomics Data For The Study O...mentioning

confidence: 99%

“…In their targeted metabolomics approach, Guo et al (2021) used the proprietary Agilent software (Agilent Technologies, United States) for peak processing. Adopting Proprietary software for the conversion of raw data into processable information is another common practice.…”

Section: Generation and Analysis Of Metabolomics Data For The Study O...mentioning

confidence: 99%

“…These modifications are probably owed to the need of a continuous flow of information between the biofilm components, and a lower requirement for defense against the outer environment than during a planktonic lifestyle. Some other specific traits in the biofilm lifestyle indicate that bacteria use siderophores (secondary metabolites) to capture preferentially available iron ( Guo et al, 2021 ). Overall, at a mature stage, these structures present a variety of microniches and environments subject to stresses that induce the development of responsive and adaptive mechanisms ( Chavez-Dozal et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

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Advances in experimental and computational methodologies for the study of microbial-surface interactions at different omics levels

et al. 2022

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The study of the biological response of microbial cells interacting with natural and synthetic interfaces has acquired a new dimension with the development and constant progress of advanced omics technologies. New methods allow the isolation and analysis of nucleic acids, proteins and metabolites from complex samples, of interest in diverse research areas, such as materials sciences, biomedical sciences, forensic sciences, biotechnology and archeology, among others. The study of the bacterial recognition and response to surface contact or the diagnosis and evolution of ancient pathogens contained in archeological tissues require, in many cases, the availability of specialized methods and tools. The current review describes advances in in vitro and in silico approaches to tackle existing challenges (e.g., low-quality sample, low amount, presence of inhibitors, chelators, etc.) in the isolation of high-quality samples and in the analysis of microbial cells at genomic, transcriptomic, proteomic and metabolomic levels, when present in complex interfaces. From the experimental point of view, tailored manual and automatized methodologies, commercial and in-house developed protocols, are described. The computational level focuses on the discussion of novel tools and approaches designed to solve associated issues, such as sample contamination, low quality reads, low coverage, etc. Finally, approaches to obtain a systems level understanding of these complex interactions by integrating multi omics datasets are presented.

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Mass spectrometry based targeted metabolomics precisely characterized new functional metabolites that regulate biofilm formation in Escherichia coli

Cited by 25 publications

References 54 publications

Secondary metabolite of Aspergillus oryzae impairs cell envelope integrity Klebsiella pneumoniae producing extended-spectrum beta-lactamase

Secondary metabolite of Aspergillus oryzae impairs cell envelope integrity Klebsiella pneumoniae producing extended-spectrum beta-lactamase

Metabolomic and cultivation insights into the tolerance of the spacecraft-associated Acinetobacter toward Kleenol 30, a cleanroom floor detergent

Advances in experimental and computational methodologies for the study of microbial-surface interactions at different omics levels

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