Mass spectrometry-based shotgun lipidomics – a critical review from the technical point of view

Hsu, Fong‐Fu

doi:10.1007/s00216-018-1252-y

Cited by 111 publications

(82 citation statements)

References 155 publications

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“…In contrast to LC-based methods that separate lipids chromatographically, shotgun lipidomics separates hundreds of lipids by electrospray ionization [16]. ESI uses electrospray under high voltage to produce multiple-charged ions [17], and therefore, shotgun lipidomics can quantify hundreds of lipids with relative simplicity of operation and short run times. However, it only allows the detection of abundant lipid species and thus has lower sensitivity than LC-based methods.…”

Section: Shotgun Lipidomicsmentioning

confidence: 99%

“…We identified 16 studies which are listed in Table S1. As to methods of lipid measurement, two early studies used shotgun lipidomics [19,20], two studies used NMR measuring total lipids, triglycerides, and cholesterol within l4 lipoprotein subclasses [21,22], and the majority of studies used LC-MS, measuring hundreds of lipids [16][17][18][19][20][21][22][23][24][25][26][27]. Eight of the 16 studies were lipidomics/metabolomics-wide association studies that assessed associations of all measured lipids with CVD outcomes [19][20][21][22][23][24][25][26], six studies used a candidate-lipid approach by focusing on specific lipids such as ceramides [27][28][29][30][31][32], and two studies classified lipids into groups and examined global associations of lipid species with CVD outcomes [33,34].…”

Section: Summary Of Recent Studies On Lipidomics For Cvdmentioning

confidence: 99%

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A Review of Lipidomics of Cardiovascular Disease Highlights the Importance of Isolating Lipoproteins

Ding

Rexrode

2020

Metabolites

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Cutting-edge lipidomic profiling measures hundreds or even thousands of lipids in plasma and is increasingly used to investigate mechanisms of cardiovascular disease (CVD). In this review, we introduce lipidomic techniques, describe distributions of lipids across lipoproteins, and summarize findings on the association of lipids with CVD based on lipidomics. The main findings of 16 cohort studies were that, independent of total and high-density lipoprotein cholesterol (HDL-c), ceramides (d18:1/16:0, d18:1/18:0, and d18:1/24:1) and phosphatidylcholines (PCs) containing saturated and monounsaturated fatty acyl chains are positively associated with risks of CVD outcomes, while PCs containing polyunsaturated fatty acyl chains (PUFA) are inversely associated with risks of CVD outcomes. Lysophosphatidylcholines (LPCs) may be positively associated with risks of CVD outcomes. Interestingly, the distributions of the identified lipids vary across lipoproteins: LPCs are primarily contained in HDLs, ceramides are mainly contained in low-density lipoproteins (LDLs), and PCs are distributed in both HDLs and LDLs. Thus, the potential mechanism behind previous findings may be related to the effect of the identified lipids on the biological functions of HDLs and LDLs. Only eight studies on the lipidomics of HDL and non-HDL particles and CVD outcomes have been conducted, which showed that higher triglycerides (TAGs), lower PUFA, lower phospholipids, and lower sphingomyelin content in HDLs might be associated with a higher risk of coronary heart disease (CHD). However, the generalizability of these studies is a major concern, given that they used case-control or cross-sectional designs in hospital settings, included a very small number of participants, and did not correct for multiple testing or adjust for blood lipids such as HDL-c, low-density lipoprotein cholesterol (LDL-c), or TAGs. Overall, findings from the literature highlight the importance of research on lipidomics of lipoproteins to enhance our understanding of the mechanism of the association between the identified lipids and the risk of CVD and allow the identification of novel lipid biomarkers in HDLs and LDLs, independent of HDL-c and LDL-c. Lipidomic techniques show the feasibility of this exciting research direction, and the lack of high-quality epidemiological studies warrants well-designed prospective cohort studies.

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Section: Shotgun Lipidomicsmentioning

confidence: 99%

Section: Summary Of Recent Studies On Lipidomics For Cvdmentioning

confidence: 99%

A Review of Lipidomics of Cardiovascular Disease Highlights the Importance of Isolating Lipoproteins

Ding

Rexrode

2020

Metabolites

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“…To most effectively remove or minimize the influence of matrix effects, the following improvements can be used: modifications to the sample extraction methodology, improved chromatographic separation, and using stable isotope-labeled internal standards (IS) [154]. Deuterium-labeled standards may have disadvantages such as different retention times as compared to analytes, undesired amplification or the weakening of ionization, while 13 C-labeled standards theoretically may be better for analysis but they are not commercially available [37,156]. The choice of the optimal amount of IS is also important, since the interaction of the analyte/internal standard affects the accuracy [157,158].…”

Section: Lc-ms/msmentioning

confidence: 99%

Methods of the Analysis of Oxylipins in Biological Samples

et al. 2020

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Oxylipins are derivatives of polyunsaturated fatty acids and due to their important and diverse functions in the body, they have become a popular subject of studies. The main challenge for researchers is their low stability and often very low concentration in samples. Therefore, in recent years there have been developments in the extraction and analysis methods of oxylipins. New approaches in extraction methods were described in our previous review. In turn, the old analysis methods have been replaced by new approaches based on mass spectrometry (MS) coupled with liquid chromatography (LC) and gas chromatography (GC), and the best of these methods allow hundreds of oxylipins to be quantitatively identified. This review presents comparative and comprehensive information on the progress of various methods used by various authors to achieve the best results in the analysis of oxylipins in biological samples.

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“…A good solution to this issue is the Nanomate nano-ESI chip developed by Advion Inc. (Ithaca, NY, USA), which relies on one nanoESI spray needle for each sample and therefore eliminates any carryover from the injection system [27]. Furthermore, nano-ESI greatly enhances signal intensities, resulting in better detection limits, and it minimizes the amount of sample needed [28]. Thus, a nano-ESI chip is considered a contemporary and highly useful piece of equipment when performing shotgun lipidomics.…”

Section: Direct Infusion Lipidomicsmentioning

confidence: 99%

Lipidomics from sample preparation to data analysis: a primer

2019

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Lipids are amongst the most important organic compounds in living organisms, where they serve as building blocks for cellular membranes as well as energy storage and signaling molecules. Lipidomics is the science of the large-scale determination of individual lipid species, and the underlying analytical technology that is used to identify and quantify the lipidome is generally mass spectrometry (MS). This review article provides an overview of the crucial steps in MS-based lipidomics workflows, including sample preparation, either liquid-liquid or solid-phase extraction, derivatization, chromatography, ion-mobility spectrometry, MS, and data processing by various software packages. The associated concepts are discussed from a technical perspective as well as in terms of their application. Furthermore, this article sheds light on recent advances in the technology used in this field and its current limitations. Particular emphasis is placed on data quality assurance and adequate data reporting; some of the most common pitfalls in lipidomics are discussed, along with how to circumvent them.

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Mass spectrometry-based shotgun lipidomics – a critical review from the technical point of view

Cited by 111 publications

References 155 publications

A Review of Lipidomics of Cardiovascular Disease Highlights the Importance of Isolating Lipoproteins

A Review of Lipidomics of Cardiovascular Disease Highlights the Importance of Isolating Lipoproteins

Methods of the Analysis of Oxylipins in Biological Samples

Lipidomics from sample preparation to data analysis: a primer

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