Mass spectrometry-based proteomics of single cells and organoids: The new generation of cancer research

Ntwali, P. V. Migisha; Heo, Chae Eun; Han, Jong Yoon; Chae, Soo Yeon; Kim, Minji; Vu, Hung M.; Kim, Min‐Sik; Kim, Hugh I.

doi:10.1016/j.trac.2020.116005

Cited by 6 publications

(3 citation statements)

References 101 publications

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“…For example, ultrasonication disrupts surface of cell membranes and the single‐cell lysate can further be treated. Another approach called minimal proteomic sample preparation (mPOP) consists of freeze/thaw cycle‐based sample processing in a microtiter plate [1, 9]. Subsequently, cell lysate is centrifuged to separate proteins from remaining interferences.…”

Section: Scp‐ms Workflowmentioning

confidence: 99%

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Automation of single‐cell proteomic sample preparation

2021

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Molecular heterogeneity exists at different spatial scales in biological samples and is an important parameter in the development of pathologies and resistances to therapies. When aiming to reach molecular heterogeneity of cells at extremely low spatial scales, single‐cell analysis can be the ultimate choice. Proteomics performed in bulk population of cells (macroproteomics) is prone to mask molecular heterogeneity. Mass spectrometry‐based single cell proteomics (SCP‐MS) is the right solution to overcome this issue. Three main problems can be identified using SCP‐MS: (i) analytical loss during sample preparation, (ii) inefficient microinjection/delivery of proteins/peptides from samples to MS and (iii) low analytical throughput. Technologies for automation of SCP have recently gained attention to improve methods accuracy, sensitivity, throughput and in‐depth and low‐biased proteome analysis. In this minireview, we therefore overview the state‐of‐the‐art of automation of SCP‐MS sample preparation approaches.

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Section: Scp‐ms Workflowmentioning

confidence: 99%

“…Analysis at SCL helps to reveal cell physio‐pathological mechanisms at the ultimate lower level of anatomical organisation [7, 8]. To the note, although highly promising in research [9], SCL tools have not been applied to clinics, for diagnostic or therapeutic decisions [8, 9]. However, Gavasso et al.…”

Section: Introductionmentioning

confidence: 99%

Automation of single‐cell proteomic sample preparation

2021

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“…2). 27 While several reviews covering the use of MS for organoid analysis have been published, 12,[28][29][30] this work focuses on metabolomics measurements in organoids using separation-and imaging-based MS methods. We describe applications beyond cancer research and drug discovery, and present some of the latest research in this rapidly evolving field.…”

Section: Introductionmentioning

confidence: 99%