Mass spectrometry-based proteomics of 3D cell culture: A useful tool to validate culture of spheroids and organoids

Avelino, Thayná Mendonça; García‐Arévalo, Marta; Torres, Felipe Rafael; Dias, Marieli M. G.; Domingues, Romênia R.; Carvalho, Murilo; Fonseca, Matheus de Castro; Rodrigues, Vanessa Kiraly Thomaz; Leme, Adriana Franco Paes; Figueira, Ana Carolina Migliorini

doi:10.1016/j.slasd.2021.10.013

Cited by 10 publications

(16 citation statements)

References 28 publications

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“…These BrAOs with optimal diameters secreted batokines which promote insulin secretion of β cells and maintained thermogenic capacity after transplantation 98 . For the magnetic levitation technique, mice SVFs 99 or preadipocytes 100 were mixed with nanoshuttles to be magnetically levitated in positive magnetophoresis for aggregation 99 , 100 . The derived WAOs recapitulated the molecular signaling pathway of WAT organogenesis 99 , 100 and complex vascularized structures through co-culture with ECs 100 .…”

Section: Strategies To Establish Adipose Organoidsmentioning

confidence: 99%

“…For the magnetic levitation technique, mice SVFs 99 or preadipocytes 100 were mixed with nanoshuttles to be magnetically levitated in positive magnetophoresis for aggregation 99 , 100 . The derived WAOs recapitulated the molecular signaling pathway of WAT organogenesis 99 , 100 and complex vascularized structures through co-culture with ECs 100 . However, unremovable nanoshuttles may induce DNA cytotoxicity once internalized 101 , 102 .…”

Section: Strategies To Establish Adipose Organoidsmentioning

confidence: 99%

“…By characterizing intermediate stages, many 'brown adipocytes' actually undergoing the SplM stage were found to be beige ones 35 , 40 , 53 , 74 , 144 . Proteomics, transcriptomics, and open chromatin analyses are also utilized for identification by comparing with native adipose tissues 35 , 99 , 145 .…”

Section: Challenges and Prospectsmentioning

confidence: 99%

“…However, current 3D adipose organoids still failed to fully recapitulate the structure and function of native adipose tissues 146 . One of the solutions is to characterize the disparities 35 , 99 and fill the gaps by improving source choices, induction recipes, and fabrication methods 66 , 77 . In addition to the aforementioned sources, adipogenic fibroblasts such as C3H10T1/2 MSCs 147 , 148 and 3T3-L1 preadipocytes 83 , 86 , 99 , 112 , 149 , human dermal fibroblasts 75 , 150 , 151 , and mature adipocytes 43 have also been applied.…”

Section: Challenges and Prospectsmentioning

confidence: 99%

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Unleashing the potential of adipose organoids: A revolutionary approach to combat obesity-related metabolic diseases

Liu,

Yang,

Yan

et al. 2024

Theranostics

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Obesity-related metabolic diseases, including obesity, diabetes, hyperlipidemia, and non-alcoholic fatty liver diseases pose a significant threat to health. However, comprehensive pathogenesis exploration and effective therapy development are impeded by the limited availability of human models. Notably, advances in organoid technology enable the generation of adipose organoids that recapitulate structures and functions of native human adipose tissues to investigate mechanisms and develop corresponding treatments for obesity-related metabolic diseases. Here, we review the general principles, sources, and three-dimensional techniques for engineering adipose organoids, along with strategies to promote maturation. We also outline the application of white adipose organoids, primarily for disease modeling and drug screening, and highlight the therapeutic potential of thermogenic beige and brown adipose organoids in promoting weight loss and glucose and lipid metabolic homeostasis. We also discuss the challenges and prospects in the establishment and bench-to-bedside of adipose organoids, as well as their potential applications.

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Section: Strategies To Establish Adipose Organoidsmentioning

confidence: 99%

Section: Strategies To Establish Adipose Organoidsmentioning

confidence: 99%

Section: Challenges and Prospectsmentioning

confidence: 99%

Section: Challenges and Prospectsmentioning

confidence: 99%

See 2 more Smart Citations

Unleashing the potential of adipose organoids: A revolutionary approach to combat obesity-related metabolic diseases

Liu,

Yang,

Yan

et al. 2024

Theranostics

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“…The consensus from these studies is that 3D cultured adipocytes show higher adipogenic gene expression, higher adiponectin secretion and, most importantly, much larger lipid droplets than 2D cultures grown in parallel [ 8 , 9 , 11 ]. In addition to the wide range of published protocols [ 12–14 ], recent publications also include a series of useful methodological papers detailing how 3D cultured adipocytes subsequently can be analysed using for example automated screening platforms, mass spectrometry, tissue clearing or transcripomics, thus opening further possibilities to use 3D cultures to generate new discoveries about basic adipocyte biology [ 11 , 15 , 16 ]. For example, a recent noteworthy study by the Laushke lab used in-depth multi-omics to identify factors that link 3D culturing to the observed enhanced adipocyte maturation [ 9 ].…”

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confidence: 99%

Towards better models for studying human adipocytes in vitro

2022

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With obesity and its comorbidities continuing to rise, we urgently need to improve our understanding of what mechanisms trigger the white adipose tissue to become dysfunctional in response to over-feeding. The recent invent of 3D culturing models has produced several noteworthy protocols for differentiating unilocular adipocytes in vitro , promising to revolutionize the obesity research field by providing more representative adipose tissue models for such mechanistic studies. In parallel, these 3D models provide important insights to how profoundly the microenvironment influences adipocyte differentiation and morphology. This commentary highlights some of the most recent 3D models, including human unilocular vascularized adipocyte spheroids (HUVASs), developed by our lab. We discuss recent developments in the field, provide further insights to the importance of the microvasculature for adipocyte maturation, and summarize what challenges remain to be solved before we can achieve a culture model that fully recapitulates all aspects of human white adipocyte biology in vitro . Taken together, the commentary highlights important recent advances regarding 3D adipocyte culturing and underlines the many advantages these models provide over traditional 2D cultures, with the aim of convincing more laboratories to switch to 3D models.

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Mass Spectrometry Imaging of Organoids to Improve Preclinical Research

Zivko,

Hahm,

Tressler

et al. 2024

Adv Healthcare Materials

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Pre‐clinical models are essential research tools before novel therapeutic or diagnostic methods can be applied to humans. These range from in vitro cell monocultures to vastly more complex animal models, but clinical translation to humans often fails to deliver significant results. Three‐dimensional organoid systems are being increasingly studied to establish physiologically relevant in vitro platforms in a trade‐off between the complexity of the research question and the complexity of practical experimental setups. The sensitivity and precision of analytical tools are yet another limiting factor in what can be investigated, and mass spectrometry is one of the most powerful analytical techniques available to the scientific community. Its innovative use to spatially resolve biological samples has opened many research avenues in the field of mass spectrometry imaging (MSI). Here, we aim to explore the current scientific landscape in the application of MSI on organoids, with an emphasis on their combined potential to facilitate and improve pre‐clinical studies.This article is protected by copyright. All rights reserved

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Mass spectrometry-based proteomics of 3D cell culture: A useful tool to validate culture of spheroids and organoids

Cited by 10 publications

References 28 publications

Unleashing the potential of adipose organoids: A revolutionary approach to combat obesity-related metabolic diseases

Unleashing the potential of adipose organoids: A revolutionary approach to combat obesity-related metabolic diseases

Towards better models for studying human adipocytes in vitro

Mass Spectrometry Imaging of Organoids to Improve Preclinical Research

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