Mass spectrometry‐based proteomic analysis of FSCN1‐interacting proteins in laryngeal squamous cell carcinoma cells

Abstract

Fascin actin-bundling protein 1 (FSCN1) is an evolutionarily conserved actinbundling protein that plays a critical role in cell migration, motility, adhesion, and cellular interactions. Although multiple clinical studies have implicated the expression of FSCN1 in laryngeal squamous cell carcinoma (LSCC) progression, the precise mechanism of FSCN1 in the process has not been clearly elucidated. To define FSCN1 function, we characterized FSCN1-interacting proteins in LSCC cells by immunoprecipitation followed by mass spectrometry (MS). After data filtering, 119 proteins with expression in both the Hep-2 and TU-177 cell samples were identified as FSCN1-interacting partners. With in-depth bioinformatics analysis, we linked FSCN1 to critical cellular processes including cell adhesion, glycolysis/gluconeogenesis, regulation of protein ubiquitination, ribosomal RNA processing, and small molecule metabolism. We discuss the interactions between FSCN1 and some of the newly validated partners. The identification of these potential partners of FSCN1 expands our knowledge of the FSCN1 interactome and provides a valuable resource for understanding the functions of this protein in LSCC progression.

The effect of fascin 1 inhibition on head and neck squamous cell carcinoma cells

“…Several studies reported an elevated fascin 1 protein expression at the tumor invasive borders in esophageal, gastric and oral carcinomas [31][32][33]. Overex-pression of fascin 1 promotes an aggressive clinical course in patients with HNSCC [8,15,16,28,37,38]. Here, we found a significant correlation between fascin 1 protein expression and HNSCC-related mortality in both univariate and multivariate analyses.…”

How does fascin promote cancer metastasis?

“…There are reports indicating that fascin might have actin‐bundling independent functions in cancer [128]. Fascin has been shown to interact with a number of proteins other than F‐actin [24,123,129–131]. Although the functional implication of these interactions in cancer metastasis is largely unclear, we suspect that fascin might have much broader roles in cancer metastasis than promoting cancer cell migration and invasion.…”

Fascin actin-bundling protein 1 in human cancer: Promising biomarker or therapeutic target?

“…In addition to the above interaction partners, various proteins have been found to physiologically interact with FSCN1. [36][37][38][39][40][41] However, it has not been established if these interactions regulate the actinbundling activity of FSCN1.…”