Mass spectrometry-based phospholipid imaging: methods and findings

Mamun, Al; Islam, Ariful; Eto, Fumihiro; Sato, Tomohito; Kahyo, Tomoaki; Setou, Mitsutoshi

doi:10.1080/14789450.2020.1880897

Cited by 11 publications

(7 citation statements)

References 137 publications

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“…In recent years, MSI has widely been used for lipidomics for detecting possible biomarkers in brain, liver, oral, breast, colorectal, skin, or other cancers [ 2 , 20 , 76 ]; in brain and neuronal tissue research [ 77 ] and models of fetal or adult cerebral ischemia [ 19 , 78 , 79 , 80 ]; in neurodegenerative disorders [ 81 ] such as Alzheimer’s disease [ 82 ] or Niemann-Pick disease [ 83 ]; as well as in cardiovascular diseases [ 84 ] such as atherosclerosis.…”

Section: Discussionmentioning

confidence: 99%

“…Mass spectrometry imaging (MSI), a rapidly growing technique in mass spectrometry (MS), allows molecular signals to be directly detected from the surface of thin biological tissues [ 1 , 2 ]. This method directly provides information about the molecular characteristics of the sample, based on the ‘mass-to-charge (m/z) ratio’ of the molecules.…”

Section: Introductionmentioning

confidence: 99%

“…Last year, almost 600 articles on protein MSI were published in the PubMed database, while the number of lipidomic MSI articles was less than 100. PLs are a large group of lipids with a wide variety of components resulting in different ionization profiles (e.g., phosphatidylcholines (PC)), typically ionized in the positive, while phosphatidylserines (PS) in negative mode and several lipids with the same m/z ratio can belong to a large number of diverse PLs [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

“…The most widely used MSI techniques for lipid determination in biological tissues are desorption electrospray ionization (DESI), matrix-assisted laser desorption ionization (MALDI) and secondary ion mass spectrometry (SIMS). A great advantage of these techniques is that the spatial distribution of individual lipids can be studied in 2D or even in 3D images [ 2 , 16 ]. Their application includes the lipid analysis in histopathological regions (e.g., atherosclerotic plaques [ 17 ], stenotic aortic valve [ 18 ], brain sections after focal cerebral ischemia [ 19 ]), the identification of potential biomarkers in tumors, as well as their potential use in diagnosis [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

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Lipid Changes in the Peri-Implantation Period with Mass Spectrometry Imaging: A Systematic Review

Gitta

Márk

Szentpéteri

et al. 2023

Life

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Mass spectrometry imaging is a sensitive method for detecting molecules in tissues in their native form. Lipids mainly act as energy stores and membrane constituents, but they also play a role in lipid signaling. Previous studies have suggested an important role of lipids in implantation; therefore, our aim was to investigate the lipid changes during this period based on the available literature. The systematic literature search was performed on Ovid MEDLINE, Cochrane Library, Embase, and LILACS. We included studies about lipid changes in the early embryonal stage of healthy mammalian development published as mass spectrometry imaging. The search retrieved 917 articles without duplicates, and five articles were included in the narrative synthesis of the results. Two articles found a different spatial distribution of lipids in the early bovine embryo and receptive uterus. Three articles investigated lipids in mice in the peri-implantation period and found a different spatial distribution of several glycerophospholipids in both embryonic and maternal tissues. Although only five studies from three different research groups were included in this systematic review, it is clear that the spatial distribution of lipids is diverse in different tissues and their distribution varies from day to day. This may be a key factor in successful implantation, but further studies are needed to elucidate the exact mechanism.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Lipid Changes in the Peri-Implantation Period with Mass Spectrometry Imaging: A Systematic Review

Gitta

Márk

Szentpéteri

et al. 2023

Life

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“…The BioMap (Novartis, Basel, Switzerland), MATLAB (Mathworks, Natick, MA, United States), Maya software (Autodesk, Inc.), FastRBF Interpolation Toolbox (FarField Technology, Inc.), Image J are most frequently used for image data processing ( Seeley and Caprioli, 2012 ). The potentiality of MSI techniques to create 3D representations of biomolecules in a whole organ or tissue with the image reconstruction process and high throughput processing has been reviewed ( Mamun et al, 2021 ). The 3D volume of brain MSI for glycome has not been investigated yet.…”

Section: Future Prospects Of Brain Msi For Glycomementioning

confidence: 99%

Mass Spectrometry Imaging for Glycome in the Brain

et al. 2021

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Glycans are diverse structured biomolecules that play crucial roles in various biological processes. Glycosylation, an enzymatic system through which various glycans are bound to proteins and lipids, is the most common and functionally crucial post-translational modification process. It is known to be associated with brain development, signal transduction, molecular trafficking, neurodegenerative disorders, psychopathologies, and brain cancers. Glycans in glycoproteins and glycolipids expressed in brain cells are involved in neuronal development, biological processes, and central nervous system maintenance. The composition and expression of glycans are known to change during those physiological processes. Therefore, imaging of glycans and the glycoconjugates in the brain regions has become a “hot” topic nowadays. Imaging techniques using lectins, antibodies, and chemical reporters are traditionally used for glycan detection. However, those techniques offer limited glycome detection. Mass spectrometry imaging (MSI) is an evolving field that combines mass spectrometry with histology allowing spatial and label-free visualization of molecules in the brain. In the last decades, several studies have employed MSI for glycome imaging in brain tissues. The current state of MSI uses on-tissue enzymatic digestion or chemical reaction to facilitate successful glycome imaging. Here, we reviewed the available literature that applied MSI techniques for glycome visualization and characterization in the brain. We also described the general methodologies for glycome MSI and discussed its potential use in the three-dimensional MSI in the brain.

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