Mass spectrometry based methods for the discrimination and typing of mycobacteria

Shitikov, Egor; Ilina, Elena N.; Черноусова, Л. Н.; Borovskaya, Alexandra D.; Рукин, И. В.; Afanas’ev, M. V.; Smirnova, Tatiana G.; Vorobyeva, Alena; Ларионова, Е. Е.; Andreevskaya, Sofya; Kostrzewa, Markus; Govorun, Vadim M.

doi:10.1016/j.meegid.2011.12.013

Cited by 44 publications

(35 citation statements)

References 27 publications

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“…The MALDI-TOF-MS whole proteome analysis conducted by Wang et al showed that proteins are useful biomolecules for the differentiation of mycobacterial strains; however, this analysis is not highly specific because of the matrix proteins and noise issues. Similar methods were used by El Khéchine et al, 2011 andShitikov et al, 2012 again, the targeted protein analysis was only applicable to previously cultured mycobacteria cells. Intact cell analysis by MALDI-TOF MS incorporates analysis of all compounds coming from the laser-ionized cell envelopes.…”

Section: Discussionmentioning

confidence: 99%

Rapid method for Mycobacterium tuberculosis identification using electrospray ionization tandem mass spectrometry analysis of mycolic acids

Szewczyk

Kowalski

Janiszewska-Drobińska³

et al. 2013

Diagnostic Microbiology and Infectious Disease

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Mycolic acids, which play a crucial role in the architecture of mycobacterial cell walls, were analyzed using electrospray ionization tandem mass spectrometry (ESI-MS/MS). A targeted analysis based on the 10 most abundant and characteristic multiple reaction monitoring (MRM) pairs was used to profile the crude fatty acid mixtures from Mtb and several nontuberculous mycobacterial strains. Comparative analysis yielded unique profiles for mycolic acids, enabling the reliable identification of mycobacterial species. In a case-control study of TB and non-TB Polish patients, we demonstrated the potential diagnostic utility of our approach for the rapid diagnosis of active TB with sensitivity and specificity surpassing those of existing methods. This robust method allows the identification of TB-positive patients after 2 h of sample preparation in the case of direct sputum analysis or 10 days of culturing, both of which are followed by one minute of LC-MS/MS analysis.

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Section: Discussionmentioning

confidence: 99%

Rapid method for Mycobacterium tuberculosis identification using electrospray ionization tandem mass spectrometry analysis of mycolic acids

Szewczyk

Kowalski

Janiszewska-Drobińska³

et al. 2013

Diagnostic Microbiology and Infectious Disease

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“…The exploitation of differences in surface proteins expressed by mycobacteria using mass spectrometry and Raman spectroscopy has showed some promise [25,26]. There needs to be further work to assess the utility of mass spectrometry as a typing tool and whether reported issues with reproducibility and lack of standardization can be overcome [27].…”

Section: Mycobacterial Diseasesmentioning

confidence: 99%

Mycobacterial Typing Systems: The Good, the Bad and the Unattainable

A¹,

connor²,

Lucey³

2016

Mycobact Dis

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“…Although several protocols for protein extraction have been proposed, there is no consensus on the best protocol to be used for the identification of mycobacteria. Furthermore, the effect of microorganism inactivation by heat has been evaluated, and it has been shown that the quality of the spectrum decreases with increasing temperature [24]. It was also recently reported that the conditions of microorganism cultivation also influence the spectrum [25] [26].…”

Section: Introductionmentioning

confidence: 99%

MALDI-TOF MS Assessment to Identify Environmental Mycobacteria

Uzam¹,

Brianesi²,

Romagnoli³

et al. 2015

AiM

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Over the past few decades, there has been a significant increase in the number of mycobacterial species described. Currently, the genus Mycobacterium consists of 170 species. Most species are called nontuberculous mycobacteria (NTM) and are potentially or rarely pathogenic and ubiquitous. One of the main challenges in mycobacteriology is the rapid and precise identification of these microorganisms. In this work, we compared two protein extraction protocols for the identification of 38 reference strains and clinical isolates, representing 27 species, by mass spectrometry (MALDI-TOF MS) to subsequently use the best method for identifying environmental mycobacteria. The results obtained with reference strains and clinical isolates showed that protocol A was effective in identifying 92.1% of mycobacterial specimens at the species level and protocol B, 50%. Therefore, protocol A was evaluated for the rapid identification of 27 environmental mycobacterial isolates. These isolates were subjected to PCR-restriction enzyme analysis (PRA-hsp65). Two isolates were misidentified by PRA-hsp65, whereas MALDI-TOF MS was able to identify them correctly. The results were confirmed by hsp65 and 16S rRNA gene sequencing. Mass spectrometry has the advantage of being a simpler and faster technique than PRA-hsp65, and our results showed that MALDI-TOF MS is a valuable tool for the identification of environmental mycobacterial isolates.

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Mass spectrometry based methods for the discrimination and typing of mycobacteria

Cited by 44 publications

References 27 publications

Rapid method for Mycobacterium tuberculosis identification using electrospray ionization tandem mass spectrometry analysis of mycolic acids

Rapid method for Mycobacterium tuberculosis identification using electrospray ionization tandem mass spectrometry analysis of mycolic acids

Mycobacterial Typing Systems: The Good, the Bad and the Unattainable

MALDI-TOF MS Assessment to Identify Environmental Mycobacteria

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