Mass-spectrometry-based metabolomics analysis for foodomics

Metabolic profiling of a range of peach fruit varieties reveals high metabolic diversity and commonalities and differences during ripening

“…Moreover, the metabolome represents the ultimate phenotype of the cells, and can also influence gene expression and protein function. Thus, metabolic profiling of fruits is a key tool to identify biomarkers related to quality and could be employed to improve breeding strategies and post-harvest storage of fresh products and optimise processing methods (Hu & Xu, 2013;Oms-Oliu, Odriozola-Serrano, & Martín-Belloso, 2013). Metabolic profiling study of primary metabolites during Dixiland peach development, using gas chromatography-mass spectrometry (GC-MS), revealed the metabolic dynamics during peach development at high resolution, and highlighted the key metabolic processes during the different peach developmental stages (Lombardo et al, 2011).…”

Non-targeted screening approaches for contaminants and adulterants in food using liquid chromatography hyphenated to high resolution mass spectrometry

“…After eluting compounds and their corresponding ions have been extracted and interpreted, this large amount of data needs to be processed efficiently, which can be challenging. A food "omics" approach attempts to identify compounds within a given sample and incorporates a similar data analysis workflow as metabolomics; this topic has been reviewed previously [69,70]. Identifying all of the compounds present in a given food sample can be quite challenging due in part to the quantity of compounds present and insufficient databases for compounds native to food matrices.…”

Future perspectives in Orbitrap™-high-resolution mass spectrometry in food analysis: a review

“…A review by Antignac et al, (2011) provides a good overview of the numerous analytical choices which have to be made at each step of any metabolomics study, and emphasises the fact that steps such as sample preparation, sample introduction, ionisation, data processing, and data analysis all have a direct impact on the final results obtained and their interpretation. Evidence continues to grow that the MS-based metabolomics is well suited to be used for characterisation and detection of the metabolome in foodstuffs related to food composition, food processing, food quality/safety, dietary supplement or intervention (Hu & Xu, 2013). The popularity of LTQOrbitrap MS has continuously grown for metabolomics applications since 2008 , Rupert et al, 2015.…”