The aim of this research was the study of the lipid and biologically active compound composition of the kernels of Prunus domestica growing in two different eco-regions of Georgia. Sums of both neutral and polar lipids were obtained from the kernels and their physical-chemical constants were determined. Fatty acid composition was studied using GC-MS and showed the content of 9-hexadecanoic, hexadecanoic, 9,12-hexadecanoic, 9-octadecanoic, octadecanoic, heptadecanoic, and eicosanoic acids, with 9,12hexadecanoic and 9-octadecanoic being the two dominants acids. The isolated fatty oils of both regions, rich in biologically active compounds, shows the potential of P. domestica oil as a cheap and easily accessible regional source for cosmetic and medicinal purposes, with the oil from the more arid Eastern region of Georgia being slightly superior in content.