Mass spectrometry and tandem mass spectrometry characterization of protein patterns, protein markers and whole proteomes for pathogenic bacteria

MALDI-TOF Mass Spectrometry Applied to Classification and Identification of Bacteria

“…The steadily growing availability of genome sequences coinciding with improved performance and also dropping costs of mass spectrometers and software will give rise to next-generation MALDI-TOF MS applications in bacteriology. Some trends for novel technologies are becoming apparent, e.g., proteome-based approaches (Intelicato-Young & Fox, 2013), S10-GERMS method (Tamura, Hotta, & Sato, 2013), SELDI-TOF MS (Dubska et al, 2011), imaging MALDI-TOF MS (Yang et al, 2012) and even beyond strain-level differentiation such as discrimination of metabolic states (Kuehl, Marten, Bischoff, Brenner-Weiss, & Obst, 2011) or tracking the transition from susceptibility to resistance against antibiotics (Kostrzewa, Sparbier, Maier, & Schubert, 2013;Shah et al, 2011). These studies raise expectations for further innovation in MALDI-TOF MS and its novel potential in microbiology.…”

Proteome-based bacterial identification using matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS): A revolutionary shift in clinical diagnostic microbiology

“…Identification of bacterial proteins using MALDI-TOF/TOF tandem mass spectrometry (MS/MS) may improve our understanding of bacterial pathogenesis [84].…”

Biomarkers for Invasive Aspergillosis: The Challenges Continue

“…The molecules are then accelerated in an electric field and, depending on their mass, travel faster or slower and so hit a detector at different times, hence the term TOF. This results in a peak pattern, the mass spectrum, which is compared with a spectrum database, allowing its identification in a matter of minutes [170]. Proteomics-targeted MALDI-TOF MS has already become an accurate identification tool in bacteriology and is now being applied to the identification of filamentous fungi at speeds relevant for fungal diagnostic procedures [171].…”