Mass‐spectrometry analysis of the human pineal proteome during night and day and in autism

Dumas, Guillaume; Goubran‐Botros, Hany; Matondo, Mariette; Pagan, Cécile; Boulègue, Cyril; Chaze, Thibault; Chamot‐Rooke, Julia; Maronde, Erik; Bourgeron, Thomas

doi:10.1111/jpi.12713

Cited by 3 publications

(1 citation statement)

References 117 publications

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“…HSP90AA1 is a member of the heat shock protein family (HSP). HSP provides protection against stress for nascent peptide chain synthesis, folding, and elimination of misfolded proteins, each of which is considered a risk factor for SZ and ASD. ,− The dysregulation of these three proteins as well as CANX (as mentioned above) and another upregulated heat shock protein TRAP1 implied an increase in protein misfolding and induced endoplasmic reticulum stress, while ASD is related to ER stress. ,, …”

Section: Discussionmentioning

confidence: 99%

iTRAQ-Based Proteomics Analysis of Rat Cerebral Cortex Exposed to Valproic Acid before Delivery

Jing

Zhang

Cao

et al. 2022

ACS Chem. Neurosci.

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Autism spectrum disorder (ASD) is a neurological and developmental disorder characterized by social and communication difficulties. Valproic acid (VPA) injection during pregnancy elicits autism-like behavior in the offspring, making it a classic animal model of ASD. However, the mechanisms involved have not yet been determined. In this study, we used iTRAQ (isobaric tags for relative and absolute quantification) proteomics analysis of the cerebral cortex of a VPA rat model (VPA group) and controls (CON group). The results showed that 79 differentially expressed proteins (DEPs) were identified between the VPA group and the CON group. Based on bioinformatics analysis, the DEPs were mainly enriched at synapses, especially glutamatergic synapses and GABAergic synapses. Some DEPs were involved in energy metabolism, thyroid hormone synthesis pathway, and Na + -K + -ATPase. Cytoskeleton and endoplasmic reticulum (ER) stress-related proteins were also involved. Some DEPs matched either the ASD gene database or previous reports on cerebral cortical transcriptome studies in VPA rat models. Dysregulation of these DEPs in the cerebral cortex of VPA rats may be responsible for autism-like behavior in rats. We also found that some DEPs were associated with neuropsychiatric disorders, implying that these diseases share common signaling pathways and mechanisms. Moreover, increased expression of DEPs was associated with energy metabolism in the cerebral cortex of VPA rats, implying that ASD may be a distinct type of mitochondrial dysfunction that requires further investigation.

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Section: Discussionmentioning

confidence: 99%

iTRAQ-Based Proteomics Analysis of Rat Cerebral Cortex Exposed to Valproic Acid before Delivery

Jing

Zhang

Cao

et al. 2022

ACS Chem. Neurosci.

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Analysis of the Human Pineal Proteome by Mass Spectrometry

2022

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Modeling Autism Spectrum Disorders with Induced Pluripotent Stem Cell-Derived Brain Organoids

et al. 2023

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Autism spectrum disorders (ASD) are a group of complex neurodevelopmental disorders that affect communication and social interactions and present with restricted interests and repetitive behavior patterns. The susceptibility to ASD is strongly influenced by genetic/heritable factors; however, there is still a large gap in understanding the cellular and molecular mechanisms underlying the neurobiology of ASD. Significant progress has been made in identifying ASD risk genes and the possible convergent pathways regulated by these gene networks during development. The breakthrough of cellular reprogramming technology has allowed the generation of induced pluripotent stem cells (iPSCs) from individuals with syndromic and idiopathic ASD, providing patient-specific cell models for mechanistic studies. In the past decade, protocols for developing brain organoids from these cells have been established, leading to significant advances in the in vitro reproducibility of the early steps of human brain development. Here, we reviewed the most relevant literature regarding the application of brain organoids to the study of ASD, providing the current state of the art, and discussing the impact of such models on the field, limitations, and opportunities for future development.

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Mass‐spectrometry analysis of the human pineal proteome during night and day and in autism

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 3 publications

References 117 publications

iTRAQ-Based Proteomics Analysis of Rat Cerebral Cortex Exposed to Valproic Acid before Delivery

iTRAQ-Based Proteomics Analysis of Rat Cerebral Cortex Exposed to Valproic Acid before Delivery

Analysis of the Human Pineal Proteome by Mass Spectrometry

Modeling Autism Spectrum Disorders with Induced Pluripotent Stem Cell-Derived Brain Organoids

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