Mass Spectrometrical Analysis of Galectin Proteins in Primary Rat Cerebellar Astrocytes

Abstract

Galectins are a family of animal lectins with specificity for beta-galactosides and are involved in a host of cellular activities, ranging from development to cancer. The molecules are expressed by neural and non-neural cells intracellularly as well as extracellularly. Using two-dimensional gel electrophoresis coupled to tandem mass spectrometry, the present work aimed to identify and characterize galectins in primary rat cerebellar astrocytes. The protein-chemical method identified nine spots representing two members of the galectin family, namely galectin-1 and galectin-3. These findings suggest that high abundant expression of galectin in astrocytes is limited to the two abundant galectin family members. As these family members are linked to human astrocytic tumors, their reliable detection in astrocytes by proteomic techniques would enable us to further understand their role in neural development, injury, and regeneration in general and astrocytoma in particular.

Efficient induction of differentiation and growth inhibition in IDH1 mutant glioma cells by the DNMT Inhibitor Decitabine

“…In TS603 glioma cells, 411 unique genes were upregulated and 82 genes were downregulated after DAC treatment (Supplemental Table 2). Compared with vehicle-treated TS603 cells, low dose transient DAC treatment resulted in the upregulation of a number of genes associated with glial-astrocytic differentiation, such as GFAP and LGALS3 [15], whereas NEUROD1 , a gene associated with neural stem cells, was downregulated (Fig. 4C).…”

Proteome reference map of the skin mucus of Atlantic cod (Gadus morhua) revealing immune competent molecules

“…The presence of galectin-1 isoforms in mucus has been reported in many vertebrates e congerin, a galectin-1 like protein from skin mucus of Conger myriaster (Japanese conger eel) has been found to separate into several isolectins on electro focusing [20]; bovine spleen and heart galectin-1 have been shown to have different tissue specific isoform profile ranging from pI 4.56 to 4.68 [21]. It is still not known whether these are biological isoforms as it has been suggested that the isolectins observed for galectin-1 may be artifacts generated when the extracellular galectin-1 gets oxidised during sample preparation [22]. The mRNA protein coding sequence of galectin-1a was cloned and the sequence submitted to Genbank (GenBank ID: HM467736).…”

Galectin-1 Enhances Astrocytic BDNF Production and Improves Functional Outcome in Rats Following Ischemia

“…However, the expression of Gal-1 in CNS is still never completely identified. Previous studies showed that it is expressed in cell body of neurons in several brain stem nucleuses and astrocytes from subventricular zone and cerebellum [12,13,24]. After injuries, Gal-1 expression seems to tightly correlate with activated astrocytes.…”