Mass spectrometric quantification of MIF-1 in mouse brain by multiple reaction monitoring

Kheterpal, Indu; Kastin, Abba J.; Mollah, Sahana; Yu, Cong; Hsuchou, Hung; Pan, Weihong

doi:10.1016/j.peptides.2009.04.004

Cited by 9 publications

(7 citation statements)

References 50 publications

(52 reference statements)

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“…As originally identified by Kastin ( Kheterpal et al, 2009 ), the N-terminal fragment of oxytocin, proline-leucine-glycine-NH 2 (OT 7-9), is also known as melanocyte-inhibiting factor-1 (MIF-1). Among the functions of MIF-1 is the regulation of pain through interactions with the opioid receptors ( Khan et al, 2010 ).…”

Section: Precursors and Fragments Of Oxytocinmentioning

confidence: 99%

Is Oxytocin “Nature’s Medicine”?

et al. 2020

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Section: Precursors and Fragments Of Oxytocinmentioning

confidence: 99%

Is Oxytocin “Nature’s Medicine”?

et al. 2020

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“…Methods for protein labeling, electrophoresis, imaging, and analysis have been described in detail previously. [14][15][16] Briefly, protein samples were covalently labeled at lysines with spectrally resolvable CyDye fluorophores (Cy2, Cy3, and Cy5). The internal control pooled sample consisting of equal amounts of protein from each sample was labeled with Cy2 and included for each gel.…”

Section: Animals and Dietsmentioning

confidence: 99%

Diet-Induced Obesity Suppresses Expression of Many Proteins at the Blood–Brain Barrier

Ouyang

Hsuchou

Kastin

et al. 2013

J Cereb Blood Flow Metab

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The blood-brain barrier (BBB) is a regulatory interface between the central nervous system and the rest of the body. However, BBB changes in obesity and metabolic syndrome have not been fully elucidated. We hypothesized that obesity reduces energy metabolism in the cerebral microvessels composing the BBB, reflected by downregulation of protein expression and function. We performed comparative proteomic analyses in enriched microvessels from the cerebral cortex of mice 2 months after ingestion of a high-fat diet or regular rodent chow. In mice with diet-induced obesity (DIO), there was downregulation of 47 proteins in the cerebral microvessels, including cytoskeletal proteins, chaperons, enzymes, transport-related proteins, and regulators for transcriptional and translational activities. Only two proteins, involved in messenger RNA (mRNA) transport and processing, were upregulated. The changes of these proteins were further validated by quantitative polymerase chain reaction (qPCR), western blotting, and immunofluorescent staining of freshly isolated microvessels, in samples obtained from different batches of mice. The predominant downregulation suggests that DIO suppresses metabolic activity of BBB microvessels. The finding of a hypometabolic state of the BBB in mice at the chronic stage of DIO is unexpected and unprecedented; it may provide novel mechanistic insight into how obesity influences CNS function via regulatory changes of the BBB. Keywords: blood-brain barrier; cerebral microvessels; high-fat diet; mass spectrometry; obesity; proteomics INTRODUCTION Obesity has become one of the most critical problems in human health and is associated with many complications of metabolic diseases. 1,2 Proteomic methods have been used to investigate human and mouse obesity, but most reports have focused on changes in adipose tissue. [3][4][5][6] Recent results suggest that the blood-brain barrier (BBB) is activated in obesity; for example, mice with adult-onset obesity show resistance to leptin transport across the BBB, region-specific astrogliosis, and upregulation of astrocytic leptin receptors. 7,8 The BBB is a regulatory interface between the central nervous system (CNS) and the peripheral circulation. It is a complex threedimensional structure composed of specialized endothelial cells that are reinforced by pericytes, astrocytic endfeet, and extracellular matrix. The tight junctions between the endothelia and the continuous basement membrane prevent diffusion of large non-lipophilic molecules between the CNS parenchyma (brain and spinal cord) and the circulation. The function of the BBB is further reinforced by a high mitochondrial content that ensures sufficient energy, an abundance of enzymes, and metabolically active astrocytes. The functions of the BBB in obesity, however, remain unclear. We predicted that BBB homeostasis is disturbed by metabolic changes in obesity.Although normal transport and secretory functions of the BBB are essential for CNS function, proteomic analyses have shown that BBB activity is ch...

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“…In recent years, this method has been modified and applied to measure concentrations of multiple NPs in different chemical environments. Kheterpal et al (76) employed stable isotopes 13 C and 15 N on the leucine residue to generate a standard curve for MIF-1 (Pro-Leu-Gly-NH 2 ), which has potent therapeutic effects in depression and Parkinson’s disease, thereby facilitating measurement of its actual concentration in the mouse brain.…”

Section: Neuropeptide Quantitationmentioning

confidence: 99%

Advances in Mass Spectrometric Tools for Probing Neuropeptides

Buchberger

2015

Annual Rev. Anal. Chem.

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Neuropeptides are important mediators in the functionality of the brain and other neurological organs. Because neuropeptides exist in a wide range of concentrations, appropriate characterization methods are needed to provide dynamic, chemical, and spatial information. Mass spectrometry and compatible tools have been a popular choice in analyzing neuropeptides. There have been several advances and challenges, both of which are the focus of this review. Discussions range from sample collection to bioinformatic tools, although avenues such as quantitation and imaging are included. Further development of the presented methods for neuropeptidomic mass spectrometric analysis is inevitable, which will lead to a further understanding of the complex interplay of neuropeptides and other signaling molecules in the nervous system.

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Mass spectrometric quantification of MIF-1 in mouse brain by multiple reaction monitoring

Cited by 9 publications

References 50 publications

Is Oxytocin “Nature’s Medicine”?

Is Oxytocin “Nature’s Medicine”?

Diet-Induced Obesity Suppresses Expression of Many Proteins at the Blood–Brain Barrier

Advances in Mass Spectrometric Tools for Probing Neuropeptides

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